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Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

《环境科学与工程前沿(英文)》 2007年 第1卷 第1期   页码 89-94 doi: 10.1007/s11783-007-0017-1

摘要: The present work studied the influence of glucose feeding on the ligninolytic enzyme production of in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium. Several sets of shaking flask experiments were conducted. The results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculation) stimulated both fungal biomass growth and enzyme production. The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was also investigated. Compared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced more enzymes. The peak and total yield of MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme was kept stable for 4 days with an activity of over 200 U/L.

关键词: enzyme production     fed-batch culture     nitrogen-limited     inoculation     mmol/L    

Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus,

Paul Olusegun Bankole, Kirk Taylor Semple, Byong-Hun Jeon, Sanjay Prabhu Govindwar

《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1292-3

摘要: Abstract • A. sydowii strain bpo1 exhibited 99.8% anthracene degradation efficiency. • Four unique metabolic products were obtained after anthracene degradation. • Ligninolytic enzymes induction played vital roles in the removal of anthracene. • Laccase played a crucial role in comparison with other enzymes induced. The present study investigated the efficiency of Aspergillus sydowii strain bpo1 (GenBank Accession Number: MK373021) in the removal of anthracene (100 mg/L). Optimal degradation efficiency (98.7%) was observed at neutral pH, temperature (30℃), biomass weight (2 g) and salinity (0.2% w/v) within 72 h. The enzyme analyses revealed 131%, 107%, and 89% induction in laccase, lignin peroxidase, and manganese peroxidase respectively during anthracene degradation. Furthermore, the degradation efficiency (99.8%) and enzyme induction were significantly enhanced with the addition of 100 mg/L of citric acid and glucose to the culture. At varying anthracene concentrations (100–500 mg/L), the degradation rate constants (k1) peaked with increasing concentration of anthracene while the half-life (t1/2) decreases with increase in anthracene concentration. Goodness of fit (R2 = 0.976 and 0.982) was observed when the experimental data were subjected to Langmuir and Temkin models respectively which affirmed the monolayer and heterogeneous nature exhibited by A. sydwoii cells during degradation. Four distinct metabolites; anthracene-1,8,9 (2H,8aH,9aH)-trione, 2,4a-dihydronaphthalene-1,5-dione, 1,3,3a,7a-tetrahydro-2-benzofuran-4,7-dione and 2-hydroxybenzoic acid was obtained through Gas Chromatography-Mass spectrometry (GC-MS). A. sydowii exhibited promising potentials in the removal of PAHs.

关键词: Aspergillus sydowii     Anthracene     Biodegradation     Polycyclic aromatic hydrocarbons    

Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus

Shuang LIU, Yanwei HOU, Guoxin SUN

《环境科学与工程前沿(英文)》 2013年 第7卷 第2期   页码 191-199 doi: 10.1007/s11783-012-0470-3

摘要: The combination of two bacteria ( sp. PY1 and sp. PY2) and a fungus ( sp. PY3), isolated from contaminated soils near a coking plant, were investigated with respect to their capability to degrade pyrene and volatilize arsenic. The results showed that all strains could use pyrene and arsenic as carbon and energy sources in a basal salts medium (BSM), with the combined potential to degrade pyrene and volatilize arsenic. sp. PY1, sp. PY2 and sp. PY3 were isolated from the consortium and were shown to degrade pyrene and volatilize arsenic independently and in combination. Fungal-bacterial coculture has shown that the most effective removal of pyrene was 96.0% and volatilized arsenic was 84.1% after incubation in liquid medium after 9 days culture, while bioremediation ability was 87.2% in contaminated soil with 100 mg·kg pyrene. The highest level of arsenic volatilization amounted to 13.9% of the initial As concentration in contaminated soil after 63 days. Therefore, a synergistic degradation system is the most effective approach to degrade pyrene and remove arsenic in contaminated soil. These findings highlight the role of these strains in the bioremediation of environments contaminated with pyrene and arsenic.

关键词: pyrene     arsenic     bioremediation     bacteria     fungus    

Progress in breeding for resistance to Ug99 and other races of the stem rust fungus in CIMMYT wheat germplasm

Sridhar BHAVANI, David P. HODSON, Julio HUERTA-ESPINO, Mandeep S. RANDHAWA, Ravi P. SINGH

《农业科学与工程前沿(英文)》 2019年 第6卷 第3期   页码 210-224 doi: 10.15302/J-FASE-2019268

摘要:

Races belonging to the Ug99 (TTKSK) lineage of the wheat stem rust fungus, carrying complex virulence combinations, and their migration to countries in Africa, Middle East and Asia continue to pose a significant threat to global wheat production. The rapid spread of additional races, e.g., TKTTF or the Digalu lineage, in several countries causing localized epidemics reminds us of the vulnerability of wheat germplasm to stem rust disease, a formidable foe referenced as early as biblical times. A global rust monitoring system reflecting increased surveillance efforts has identified 13 races within the Ug99 lineage in 13 countries and unrelated lineages are emerging, spreading and posing serious threats to wheat production. Race TKTTF has caused localized epidemics in Ethiopia and its variants have been recently implicated in stem rust outbreaks in Europe. Concerted research efforts have resulted in the identification of several new resistance (APR) genes and gene combinations for use in breeding. Combining multiple adult plant resistance genes in high-yielding backgrounds and discovery of new quantitative trait loci conferring stem rust resistance has progressed in the recent years, enhancing the durability of resistance. Effective gene stewardship and new generation breeding materials and cultivars that combine multiple race-specific or minor to intermediate effect APR genes, complemented by active surveillance and monitoring, have helped to limit major epidemics and increase grain yield potential in key target environments.

关键词: adult plant resistance     black rust     race-specific resistance     Triticum aestivum    

Removal of dyes from wastewater by growing fungal pellets in a semi-continuous mode

Tao Lu, Qilei Zhang, Shanjing Yao

《化学科学与工程前沿(英文)》 2017年 第11卷 第3期   页码 338-345 doi: 10.1007/s11705-017-1644-0

摘要: To increase the efficiency of dye removal from wastewater using mycelial pellets, a bubble column reactor with a simple structure was designed and efficiently used to remove dyes from solution containing dyes. The mycelial pellets were prepared by marine fungus ZJUBE-1. Eight dyes were tested as dye targets for the adsorption capacity of mycelial pellets and good removal results were obtained. Eriochrome black T was selected as a model dye for characterizing the adsorption processes in detail. The measurement results of Zeta potential and FT-IR analysis indicate that the electrostatic attraction may play a key role in the biosorption process. The bubble column reactor was utilized to study the batch dye-removal efficiency of mycelial pellets. A re-culture process between every two batches, which was under non-sterile condition, successfully enhanced the utilization of mycelium biomass. The dye removal rate is 96.4% after 12 h in the first batch and then decreases slowly in the following batches. This semi-continuous mode, which consists of commutative processes of dye-removal and re-culture, has some outstanding advantages, such as low power consumption, easy operation, high dye removal rate, and efficient biomass utilization.

关键词: dye     mycelial pellets     marine fungus     bubble column reactor     semi-continuous biosorption    

New insights in the battle between wheat and

Chunlei TANG,Xiaojie WANG,Yulin CHENG,Minjie LIU,Mengxin ZHAO,Jinping WEI,Zhensheng KANG

《农业科学与工程前沿(英文)》 2015年 第2卷 第2期   页码 101-114 doi: 10.15302/J-FASE-2015068

摘要: Wheat stripe rust caused by f. sp. ( ) poses a great threat to wheat production worldwide. The rapid change in virulence of leads to a loss of resistance in currently resistant wheat cultivars, which results in frequent disease epidemics. Therefore, a major focus is currently placed on investigating the molecular mechanisms underlying this rapid variation of pathogenicity and coevolving wheat resistance. Limited by the lack of a system for stable transformation of and the difficulties in wheat transformation, it is not easy to generate deeper insights into the wheat- interaction using established genetic methods. Nevertheless, considerable effort has been made to unravel the wheat- interaction and significant progress is being made. Histology and cytology have revealed basic details of infection strategies and defense responses during wheat- interactions, identified cellular components involved in wheat- interactions, and have helped to elucidate their role in the infection process or in plant defense responses. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat- pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance response and in virulence. Studies of wheat- interactions have now entered a new phase in which cellular and molecular approaches are being used. This review focuses on the cellular biology of wheat- interactions and integrates the emerging data from molecular analyses with the histocytological observations.

关键词: wheat     strip rust     Puccinia striiformis f. sp. tritici     host defense     pathogen virulence     biotrophic fungus    

Trophic mode and organics metabolic characteristic of fungal community in swine manure composting

Jing Peng, Ke Wang, Xiangbo Yin, Xiaoqing Yin, Mengfei Du, Yingzhi Gao, Philip Antwi, Nanqi Ren, Aijie Wang

《环境科学与工程前沿(英文)》 2019年 第13卷 第6期 doi: 10.1007/s11783-019-1177-5

摘要: Fungal trophic modes and substrates utilization ability was observed in composting. Fungi had the higher diversity and more trophic types in thermophilic phase. Fungi had the higher metabolic potential in fresh swine manure and mature production. Redox potential, organics and moisture are main factors impacting fungal community. Composting reduced pathogenic fungi and enrich dung saprotroph fungi in swine manure. The succession of fungal community, trophic mode and metabolic characteristics were evaluated in 60 days composting of swine manure by high-throughput sequencing, FUNGuild and Biolog method, respectively. The result showed that the fungal community diversity reached to the highest level (76 OTUs) in the thermophilic phase of composting, then sustained decline to 15 OTUs after incubation. There were 10 fungal function groups in the raw swine manure. Pathotroph-saprotroph fungi reached to 15.91% on Day-10 but disappeared on Day-60. Dung saprotroph-undefined saprotroph fungi grown from 0.19% to 52.39% during the treatment. The fungal community had more functional groups but the lower substrate degradation rates in the thermophilic phase. The fungal communities on Day-0 and Day-60 had the highest degradation rates of amino acids and polymers, respectively. Redundancy analysis showed that ORP (49.6%), VS/Ash (45.3%) and moisture (39.2%) were the main influence factors on the succession of fungal community in the swine manure composting process.

关键词: Fungus     FUNGuild     Biolog     Trophic mode     Composting     Oxidation reduction potential    

中国食用菌产业技术对非合作与发展研究

李芬妮 ,张俊飚 ,姚方杰, 傅廷栋

《中国工程科学》 2019年 第21卷 第4期   页码 105-110 doi: 10.15302/J-SSCAE-2019.04.009

摘要:

农业技术落后与缺乏严重阻碍了非洲农业的发展,我国作为农耕历史文化悠久以及负责任大国,通过输出实用生产技术实现中非合作是值得思考的问题。企业是中国农业“走出去”的主体,本文以吉林省海外农业投资开发集团有限公司为例,发现基于我国食用菌产业实力雄厚、优化大国形象的需要以及非洲自身的资源和市场优势使得中国食用菌产业技术对非拓展市场成为可能。本文提出了加大选育适合当地的当家品种、控制生产规模扩张速度、加强技术人才的培养、充分运用国家相关优惠政策等建议,以推动我国食用菌产业技术对非合作与发展。

关键词: 食用菌     中非合作     “一带一路”     农业合作    

Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of

Jin Zhao, Qing-Xi Wu, Xiao-Du Cheng, Ting Su, Xiao-Hui Wang, Wen-Na Zhang, Yong-Ming Lu, Yan Chen

《化学科学与工程前沿(英文)》 2021年 第15卷 第2期   页码 421-436 doi: 10.1007/s11705-020-1952-7

摘要: Fungi play an important role in dying wastewater treatment. In this work, the mycelia of exhibited an excellent capacity in decolorizing coomassie brilliant blue (CBB). The results demonstrated that the mycelia could treat CBB with high concentrations over a broad range of pH and temperature. The decolorization rate of 99.19% and the removal rate of 16.31 mg·L ·h were realized. The mycelia could be recycled from decolorizing process for 19 times, indicating a good re-usability. It verified that the lignin peroxidase (121.65 U·L ) and manganese peroxidase (36.77 U·L ) were involved in the degradation and decolorization process of CBB. Toxicity assessments indicated the seed germination rate was up to 82.22% while inhibition to decreased dramatically and no significant effect on growth was found. The removal of CBB was a synergistic process accomplished by adsorption and biodegradation. The mycelia could be used for eco-friendly CBB treatment.

关键词: fungus mycelia     biodegradation     extracellular enzymes     coomassie brilliant blue     Lactarius deliciosus    

标题 作者 时间 类型 操作

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

期刊论文

Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus,

Paul Olusegun Bankole, Kirk Taylor Semple, Byong-Hun Jeon, Sanjay Prabhu Govindwar

期刊论文

Synergistic degradation of pyrene and volatilization of arsenic by cocultures of bacteria and a fungus

Shuang LIU, Yanwei HOU, Guoxin SUN

期刊论文

Progress in breeding for resistance to Ug99 and other races of the stem rust fungus in CIMMYT wheat germplasm

Sridhar BHAVANI, David P. HODSON, Julio HUERTA-ESPINO, Mandeep S. RANDHAWA, Ravi P. SINGH

期刊论文

Removal of dyes from wastewater by growing fungal pellets in a semi-continuous mode

Tao Lu, Qilei Zhang, Shanjing Yao

期刊论文

New insights in the battle between wheat and

Chunlei TANG,Xiaojie WANG,Yulin CHENG,Minjie LIU,Mengxin ZHAO,Jinping WEI,Zhensheng KANG

期刊论文

Trophic mode and organics metabolic characteristic of fungal community in swine manure composting

Jing Peng, Ke Wang, Xiangbo Yin, Xiaoqing Yin, Mengfei Du, Yingzhi Gao, Philip Antwi, Nanqi Ren, Aijie Wang

期刊论文

中国食用菌产业技术对非合作与发展研究

李芬妮 ,张俊飚 ,姚方杰, 傅廷栋

期刊论文

Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of

Jin Zhao, Qing-Xi Wu, Xiao-Du Cheng, Ting Su, Xiao-Hui Wang, Wen-Na Zhang, Yong-Ming Lu, Yan Chen

期刊论文