资源类型

期刊论文 1

年份

2018 1

关键词

检索范围：

排序： 展示方式：

Fusaricidins in

Weicheng LIU, Xiaoli WU, Xuelian BAI, Hong ZHANG, Dan DONG, Taotao ZHANG, Huiling WU

《农业科学与工程前沿（英文）》 2018年 第5卷 第2期   页码 262-270 doi: 10.15302/J-FASE-2017166

摘要： Four kinds of antifungal compounds from an extract of A21 with molecular masses of 883.56, 897.59, 947.55, and 961.58 Da were characterized as the members of fusaricidin-type of antibiotics according to LC-MS analysis. Fusaricidins isolated from culture filtrate displayed high antagonistic activity against several plant fungal pathogens, especially , the causal agent of gray mold. The fusaricidins biosynthetic gene cluster (BGC) from A21 was cloned by PCR and comparative cluster analysis revealed that gene , the 3′ boundary of the fusaricidin BGC in strain PKB1, was not present in fusaricidin BGC of A21, indicating that is not necessary for fusaricidin synthesis. Fusaricidin extract from A21 significantly reduced gray mold disease incidence and severity on tomato. The mRNA levels for three patho-genesis-related proteins (PRs) revealed that treatment of tomato leaves with fusaricidin extract induced the expression of PR genes to different levels, suggesting that one reason for the reduction of gray mold infection by fusaricidin is induction of PR proteins, which lead to increased resistance to pathogens. This is the first report of the application of fusaricidins to control tomato gray mold and the comparative cluster analysis provides important molecular basis for research on fusaricidin biosynthesis.

关键词： antifungal activity     biosynthetic gene cluster     Botrytis cinerea     fusaricidin     Paenibacillus polymyxa    

HTML PDF 收藏

标题 作者 时间 类型 操作

Fusaricidins in

Weicheng LIU, Xiaoli WU, Xuelian BAI, Hong ZHANG, Dan DONG, Taotao ZHANG, Huiling WU

期刊论文