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Evaluation of molecular residual disease in operable non-small cell lung cancer with gene fusions, exon

《医学前沿(英文)》 doi: 10.1007/s11684-024-1060-z

摘要: Gene fusions and MET alterations are rare and difficult to detect in plasma samples. The clinical detection efficacy of molecular residual disease (MRD) based on circulating tumor DNA (ctDNA) in patients with non-small cell lung cancer (NSCLC) with these mutations remains unknown. This prospective, non-intervention study recruited 49 patients with operable NSCLC with actionable gene fusions (ALK, ROS1, RET, and FGFR1), MET exon 14 skipping or de novo MET amplification. We analyzed 43 tumor tissues and 111 serial perioperative plasma samples using 1021- and 338-gene panels, respectively. Detectable MRD correlated with a significantly higher recurrence rate (P < 0.001), yielding positive predictive values of 100% and 90.9%, and negative predictive values of 82.4% and 86.4% at landmark and longitudinal time points, respectively. Patients with detectable MRD showed reduced disease-free survival (DFS) compared to those with undetectable MRD (P < 0.001). Patients who harbored tissue-derived fusion/MET alterations in their MRD had reduced DFS compared to those who did not (P = 0.05). To our knowledge, this is the first comprehensive study on ctDNA-MRD clinical detection efficacy in operable NSCLC patients with gene fusions and MET alterations. Patients with detectable tissue-derived fusion/MET alterations in postoperative MRD had worse clinical outcomes.

关键词: ctDNA     molecular residual disease     operable NSCLC     gene fusion     MET exon skipping     MET amplification    

Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome

null

《医学前沿(英文)》 2013年 第7卷 第3期   页码 280-289 doi: 10.1007/s11684-013-0265-3

摘要:

Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies. In recent years, novel gene fusions have been identified in cases without prior knowledge of the genetic background. Accompanied by a powerful computational data analysis method, new genome-wide screening approaches were used to detect cryptic genomic aberrations. This review focused on advanced genome-wide screening approaches in fusion gene identification, such as microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression system. The fundamental rationale and strategy for fusion gene identification using each biotech platform are also discussed.

关键词: gene fusion     cancer     microarray     next-generation sequencing     NanoString nCounter system    

Magnetic confinement fusion: a brief review

Chuanjun HUANG, Laifeng LI

《能源前沿(英文)》 2018年 第12卷 第2期   页码 305-313 doi: 10.1007/s11708-018-0539-1

摘要: Fusion energy is considered to be the ultimate energy source, which does not contribute to climate change compared with conventional fossil fuel. It is massive compared with unconventional renewable energy and demonstrates fewer safety features compared with unconventional fission energy. During the past several decades, never-ceasing efforts have been made to peacefully utilize the fusion energy in various approaches, especially inertial confinement and magnetic confinement. In this paper, the main developments of magnetic confinement fusion with emphasis on confinement systems as well as challenges of materials related to superconducting magnet and plasma-facing components are reviewed. The scientific feasibility of magnetic confinement fusion has been demonstrated in JET, TFTR, JT-60, and EAST, which instigates the construction of the International Thermonuclear Experimental Reactor (ITER). A fusion roadmap to DEMO and commercial fusion power plant has been established and steady progresses have been made to achieve the ultimate energy source.

关键词: fusion energy     magnetic confinement     tokamak     structural material     superconducting magnet    

Turbidity-adaptive underwater image enhancement method using image fusion

《机械工程前沿(英文)》 2022年 第17卷 第3期 doi: 10.1007/s11465-021-0669-8

摘要: Clear, correct imaging is a prerequisite for underwater operations. In real freshwater environment including rivers and lakes, the water bodies are usually turbid and dynamic, which brings extra troubles to quality of imaging due to color deviation and suspended particulate. Most of the existing underwater imaging methods focus on relatively clear underwater environment, it is uncertain that if those methods can work well in turbid and dynamic underwater environments. In this paper, we propose a turbidity-adaptive underwater image enhancement method. To deal with attenuation and scattering of varying degree, the turbidity is detected by the histogram of images. Based on the detection result, different image enhancement strategies are designed to deal with the problem of color deviation and blurring. The proposed method is verified by an underwater image dataset captured in real underwater environment. The result is evaluated by image metrics including structure similarity index measure, underwater color image quality evaluation metric, and speeded-up robust features. Test results exhibit that the method can correct the color deviation and improve the quality of underwater images.

关键词: turbidity     underwater image enhancement     image fusion     underwater robots     visibility    

M-LFM: a multi-level fusion modeling method for shape−performance integrated digital twin of complex

《机械工程前沿(英文)》 2022年 第17卷 第4期 doi: 10.1007/s11465-022-0708-0

摘要: As a virtual representation of a specific physical asset, the digital twin has great potential for realizing the life cycle maintenance management of a dynamic system. Nevertheless, the dynamic stress concentration is generated since the state of the dynamic system changes over time. This generation of dynamic stress concentration has hindered the exploitation of the digital twin to reflect the dynamic behaviors of systems in practical engineering applications. In this context, this paper is interested in achieving real-time performance prediction of dynamic systems by developing a new digital twin framework that includes simulation data, measuring data, multi-level fusion modeling (M-LFM), visualization techniques, and fatigue analysis. To leverage its capacity, the M-LFM method combines the advantages of different surrogate models and integrates simulation and measured data, which can improve the prediction accuracy of dynamic stress concentration. A telescopic boom crane is used as an example to verify the proposed framework for stress prediction and fatigue analysis of the complex dynamic system. The results show that the M-LFM method has better performance in the computational efficiency and calculation accuracy of the stress prediction compared with the polynomial response surface method and the kriging method. In other words, the proposed framework can leverage the advantages of digital twins in a dynamic system: damage monitoring, safety assessment, and other aspects and then promote the development of digital twins in industrial fields.

关键词: shape−performance integrated digital twin (SPI-DT)     multi-level fusion modeling (M-LFM)     surrogate model     telescopic boom crane     data fusion    

Gradient-based compressive image fusion

Yang CHEN,Zheng QIN

《信息与电子工程前沿(英文)》 2015年 第16卷 第3期   页码 227-237 doi: 10.1631/FITEE.1400217

摘要: We present a novel image fusion scheme based on gradient and scrambled block Hadamard ensemble (SBHE) sampling for compressive sensing imaging. First, source images are compressed by compressive sensing, to facilitate the transmission of the sensor. In the fusion phase, the image gradient is calculated to reflect the abundance of its contour information. By compositing the gradient of each image, gradient-based weights are obtained, with which compressive sensing coefficients are achieved. Finally, inverse transformation is applied to the coefficients derived from fusion, and the fused image is obtained. Information entropy (IE), Xydeas’s and Piella’s metrics are applied as non-reference objective metrics to evaluate the fusion quality in line with different fusion schemes. In addition, different image fusion application scenarios are applied to explore the scenario adaptability of the proposed scheme. Simulation results demonstrate that the gradient-based scheme has the best performance, in terms of both subjective judgment and objective metrics. Furthermore, the gradient-based fusion scheme proposed in this paper can be applied in different fusion scenarios.

关键词: Compressive sensing (CS)     Image fusion     Gradient-based image fusion     CS-based image fusion    

Information fusion in aquaculture: a state-of the art review

Shahbaz Gul HASSAN,Murtaza HASAN,Daoliang LI

《农业科学与工程前沿(英文)》 2016年 第3卷 第3期   页码 206-221 doi: 10.15302/J-FASE-2016111

摘要: Efficient fish feeding is currently one of biggest challenges in aquaculture to enhance the production of fish quality and quantity. In this review, an information fusion approach was used to integrate multi-sensor and computer vision techniques to make fish feeding more efficient and accurate. Information fusion is a well-known technology that has been used in different fields of artificial intelligence, robotics, image processing, computer vision, sensors and wireless sensor networks. Information fusion in aquaculture is a growing field of research that is used to enhance the performance of an “industrialized” ecosystem. This review study surveys different fish feeding systems using multi-sensor data fusion, computer vision technology, and different food intake models. In addition, different fish behavior monitoring techniques are discussed, and the parameters of water, pH, dissolved oxygen, turbidity, temperature etc., necessary for the fish feeding process, are examined. Moreover, the different waste management and fish disease diagnosis techniques using different technologies, expert systems and modeling are also reviewed.

关键词: aquaculture     computer vision     information fusion     modeling     sensor    

Iterative HOEO fusion strategy: a promising tool for enhancing bearing fault feature

《机械工程前沿(英文)》 2023年 第18卷 第1期 doi: 10.1007/s11465-022-0725-z

摘要: As parameter independent yet simple techniques, the energy operator (EO) and its variants have received considerable attention in the field of bearing fault feature detection. However, the performances of these improved EO techniques are subjected to the limited number of EOs, and they cannot reflect the non-linearity of the machinery dynamic systems and affect the noise reduction. As a result, the fault-related transients strengthened by these improved EO techniques are still subject to contamination of strong noises. To address these issues, this paper presents a novel EO fusion strategy for enhancing the bearing fault feature nonlinearly and effectively. Specifically, the proposed strategy is conducted through the following three steps. First, a multi-dimensional information matrix (MDIM) is constructed by performing the higher order energy operator (HOEO) on the analysis signal iteratively. MDIM is regarded as the fusion source of the proposed strategy with the properties of improving the signal-to-interference ratio and suppressing the noise in the low-frequency region. Second, an enhanced manifold learning algorithm is performed on the normalized MDIM to extract the intrinsic manifolds correlated with the fault-related impulses. Third, the intrinsic manifolds are weighted to recover the fault-related transients. Simulation studies and experimental verifications confirm that the proposed strategy is more effective for enhancing the bearing fault feature than the existing methods, including HOEOs, the weighting HOEO fusion, the fast Kurtogram, and the empirical mode decomposition.

关键词: higher order energy operator     fault diagnosis     manifold learning     rolling element bearing     information fusion    

Contact detection with multi-information fusion for quadruped robot locomotion under unstructured terrain

《机械工程前沿(英文)》 2023年 第18卷 第3期 doi: 10.1007/s11465-023-0760-4

摘要: Reliable foot-to-ground contact state detection is crucial for the locomotion control of quadruped robots in unstructured environments. To improve the reliability and accuracy of contact detection for quadruped robots, a detection approach based on the probabilistic contact model with multi-information fusion is presented to detect the actual contact states of robotic feet with the ground. Moreover, a relevant control strategy to address unexpected early and delayed contacts is planned. The approach combines the internal state information of the robot with the measurements from external sensors mounted on the legs and feet of the prototype. The overall contact states are obtained by the classification of the model-based predicted probabilities. The control strategy for unexpected foot-to-ground contacts can correct the control actions of each leg of the robot to traverse cluttered environments by changing the contact state. The probabilistic model parameters are determined by testing on the single-leg experimental platform. The experiments are conducted on the experimental prototype, and results validate the contact detection and control strategy for unexpected contacts in unstructured terrains during walking and trotting. Compared with the body orientation under the time-based control method regardless of terrain, the root mean square errors of roll, pitch, and yaw respectively decreased by 60.07%, 54.73%, and 64.50% during walking and 73.40%, 61.49%, and 61.48% during trotting.

关键词: multi-information fusion     contact detection     quadruped robot     probabilistic contact model     unstructured terrain    

Analysis of spinal lumbar interbody fusion cage subsidence using Taguchi method, finite element analysis

Christopher John NASSAU, N. Scott LITOFSKY, Yuyi LIN

《机械工程前沿(英文)》 2012年 第7卷 第3期   页码 247-255 doi: 10.1007/s11465-012-0335-2

摘要:

Subsidence, when implant penetration induces failure of the vertebral body, occurs commonly after spinal reconstruction. Anterior lumbar interbody fusion (ALIF) cages may subside into the vertebral body and lead to kyphotic deformity. No previous studies have utilized an artificial neural network (ANN) for the design of a spinal interbody fusion cage. In this study, the neural network was applied after initiation from a Taguchi L18 orthogonal design array. Three-dimensional finite element analysis (FEA) was performed to address the resistance to subsidence based on the design changes of the material and cage contact region, including design of the ridges and size of the graft area. The calculated subsidence is derived from the ANN objective function which is defined as the resulting maximum von Mises stress (VMS) on the surface of a simulated bone body after axial compressive loading. The ANN was found to have minimized the bone surface VMS, thereby optimizing the ALIF cage given the design space. Therefore, the Taguchi-FEA-ANN approach can serve as an effective procedure for designing a spinal fusion cage and improving the biomechanical properties.

关键词: anterior lumbar interbody fusion (ALIF)     artificial neural network (ANN)     finite element     interbody cage     lumbar interbody fusion     subsidence     taguchi method    

人类遗传病的家系收集疾病基因定位克隆与疾病基因功能的研究

夏家辉

《中国工程科学》 2000年 第2卷 第11期   页码 1-11

摘要:

介绍了中国医学遗传学国家重点实验室在遗传病家系收集、疾病基因定位、疾病基因克隆和疾病基因功能研究方面的研究工作。用细胞遗传学G显带技术于1975年发现了一条与鼻咽癌相关的标记染色体t(1;3)(q44;p11);1981年将睾丸决定基因(TDF)定位于Yp11.32带;1991年以来收集遗传病家系345种共590个;1996年用显微切割、PCR、微克隆技术克隆了EXT2基因;1998年用基因家族-候选疾病基因克隆方法克隆了遗传性神经性耳聋基因GJB3;1999年用连锁分析和全基因组扫描将一种遗传性弥漫性浅表性光敏性汗孔角化症定位于12q23.2带,并在基因功能研究中发现了一个新的细胞内转运蛋白。

关键词: 遗传病家系     基因定位和克隆     基因家族-候选疾病基因克隆     基因组扫描     基因功能研究    

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

《化学科学与工程前沿(英文)》 2017年 第11卷 第4期   页码 663-675 doi: 10.1007/s11705-017-1623-5

摘要: Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as long as they can be delivered safely and effectively into cells. Dendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecture and multivalent cooperativity. However, the clinical translation of RNA therapeutics mediated by dendrimer delivery is hampered by the lack of dendrimers that are of high quality to meet good manufacturing practice standard. In this context, we have developed small amphiphilic dendrimers that self-assemble into supramolecular structures, which mimic high-generation dendrimers synthesized with covalent construction, yet are easy to produce in large amount and superior quality. Indeed, the concept of supramolecular dendrimers has proved to be very promising, and has opened up a new avenue for dendrimer-mediated siRNA delivery. A series of self-assembling supramolecular dendrimers have consequently been established, some of them out-performing the currently available nonviral vectors in delivering siRNA to various cell types and , including human primary cells and stem cells. This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery and the advantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNA delivery. We then highlight our efforts in creating self-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA delivery as well as the related structural alterations to enhance delivery efficiency. The advent of self-assembling supramolecular dendrimer nanovectors holds great promise and heralds a new era of dendrimer-mediated delivery of RNA therapeutics in biomedical applications.

关键词: gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

Development and testing of a wireless smart toolholder with multi-sensor fusion

《机械工程前沿(英文)》 2023年 第18卷 第4期 doi: 10.1007/s11465-023-0774-y

摘要: The smart toolholder is the core component in the development of intelligent and precise manufacturing. It enables in situ monitoring of cutting data and machining accuracy evolution and has become a focal point in academic research and industrial applications. However, current table and rotational dynamometers for milling force, vibration, and temperature testing suffer from cumbersome installation and provide only a single acquisition signal, which limits their use in laboratory settings. In this study, we propose a wireless smart toolholder with multi-sensor fusion for simultaneous sensing of milling force, vibration, and temperature signals. We select force, vibration, and temperature sensors suitable for smart toolholder fusion to adapt to the cutting environment. Thereafter, structural design, circular runout, dynamic balancing, static stiffness, and dynamic inherent frequency tests are conducted to assess its dynamic and static performance. Finally, the smart toolholder is tested for accuracy and repeatability in terms of force, vibration, and temperature. Experimental results demonstrate that the smart toolholder accurately captures machining data with a relative deviation of less than 1.5% compared with existing force gauges and provides high repeatability of milling temperature and vibration signals. Therefore, it is a smart solution for machining condition monitoring.

关键词: wireless smart toolholder     multi-sensor fusion     circular runout     dynamic balancing     static stiffness     dynamic inherent frequency    

The antibiotic resistome: gene flow in environments, animals and human beings

null

《医学前沿(英文)》 2017年 第11卷 第2期   页码 161-168 doi: 10.1007/s11684-017-0531-x

摘要:

The antibiotic resistance is natural in bacteria and predates the human use of antibiotics. Numerous antibiotic resistance genes (ARGs) have been discovered to confer resistance to a wide range of antibiotics. The ARGs in natural environments are highly integrated and tightly regulated in specific bacterial metabolic networks. However, the antibiotic selection pressure conferred by the use of antibiotics in both human medicine and agriculture practice leads to a significant increase of antibiotic resistance and a steady accumulation of ARGs in bacteria. In this review, we summarized, with an emphasis on an ecological point of view, the important research progress regarding the collective ARGs (antibiotic resistome) in bacterial communities of natural environments, human and animals, i.e., in the one health settings. We propose that the resistance gene flow in nature is “from the natural environments” and “to the natural environments”; human and animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene “circulation.”

关键词: antibiotic resistance     resistome     microbiome     gene flow    

Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

《医学前沿(英文)》 2009年 第3卷 第2期   页码 141-147 doi: 10.1007/s11684-009-0041-6

摘要: Rab proteins and their effectors facilitate vesicular transport by tethering donor vesicles to their respective target membranes. Rab9 mediates late endosome-to- -Golgi-network trafficking. To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed Lentivirus encoding Rab9. The expressing plasmid pCDH1-MCF1-Rab9-EF1-copGFP was constructed by using molecular biological techniques. The Lentivirus encoding Rab9 cDNA was packed by Lifectamine-2000 mediated co-transfection of the plasmid pPACKH1- , pPACKH1- and pVSV- into 293T cells. DNA sequencing proved the successful construction of pCDH1-MCF1-Rab9-EF1-copGFP. After 72 hours, the expression of GFP could be detected in BV-2 cells. Western blotting revealed that the Rab9 gene expression in BALB/c mice brain was up-regulated significantly 4 weeks after injection with Lentivirus encoding Rab9, which evidenced a satisfactory increasing effect of this virus. Administration of Lenti-Rab9 to postnatal day 3 Niemann-Pick disease type C (NPC) mice reduced motor defects and prevented the weight loss associated with female NPC mice, as well as modulating the death rate of Purkinje neurons. It is concluded that the packaging of Lentivirus encoding Rab9 was successful. Lentivirus encoding Rab9 can increase the expression of Rab9 gene effectively, which might offer a novel means for the treatment of neurodegenerative diseases.

关键词: Rab9     lentivirus     gene therapy     gene transfer    

标题 作者 时间 类型 操作

Evaluation of molecular residual disease in operable non-small cell lung cancer with gene fusions, exon

期刊论文

Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome

null

期刊论文

Magnetic confinement fusion: a brief review

Chuanjun HUANG, Laifeng LI

期刊论文

Turbidity-adaptive underwater image enhancement method using image fusion

期刊论文

M-LFM: a multi-level fusion modeling method for shape−performance integrated digital twin of complex

期刊论文

Gradient-based compressive image fusion

Yang CHEN,Zheng QIN

期刊论文

Information fusion in aquaculture: a state-of the art review

Shahbaz Gul HASSAN,Murtaza HASAN,Daoliang LI

期刊论文

Iterative HOEO fusion strategy: a promising tool for enhancing bearing fault feature

期刊论文

Contact detection with multi-information fusion for quadruped robot locomotion under unstructured terrain

期刊论文

Analysis of spinal lumbar interbody fusion cage subsidence using Taguchi method, finite element analysis

Christopher John NASSAU, N. Scott LITOFSKY, Yuyi LIN

期刊论文

人类遗传病的家系收集疾病基因定位克隆与疾病基因功能的研究

夏家辉

期刊论文

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

期刊论文

Development and testing of a wireless smart toolholder with multi-sensor fusion

期刊论文

The antibiotic resistome: gene flow in environments, animals and human beings

null

期刊论文

Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

期刊论文