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Genotype variations in accumulation of cadmium and lead in celery (

Kun ZHANG, Jianbing WANG, Zhongyi YANG, Guorong XIN, Jiangang YUAN, Junliang XIN, Charlie HUANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 85-96 doi: 10.1007/s11783-012-0399-3

摘要： To help reduce risks of heavy metal pollution, two pot experiments were conducted to investigate the variations, transfer potential, and stability of Cadmium (Cd) and Lead (Pb) accumulations in celery ( L.) and to screen for low Cd and Pb accumulative cultivars. The maximum differences in shoot Cd concentration were 4.7-fold under low-Cd exposure and 3.3-fold under high-Cd exposure. These genotype variations in Cd accumulation are sufficiently large to help reduce Cd contamination risk in soil by using the Low-Cd-Accumulative genotypes. Cd accumulation of the Low-Cd-Accumulative genotypes is significantly positive correlated with Pb accumulation. Evidence obtained proves that Cd and Pb accumulations in celery are stable and genotype-dependent at the cultivar level. The presence of high-Pb contamination in soil promoted Cd accumulation in shoots of celery. Celery is considered a species with high risks in Cd pollution and low risks in Pb pollution. Among the tested cultivars, cv. Shuanggangkangbing (SGKB) had the lowest shoot Cd and Pb accumulating abilities, and thus is the most important material for breeding of pollution-safe cultivars (PSCs) to minimize Cd and Pb accumulations in celery.

关键词： cadmium (Cd) accumulation     lead (Pb) accumulation     celery     genotype variation     food safety    

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p53 functional activation is independent of its genotype in five esophageal squamous cell carcinoma cell

Junfang JI, Kun WU, Min WU, Qimin ZHAN,

《医学前沿（英文）》 2010年 第4卷 第4期   页码 412-418 doi: 10.1007/s11684-010-0260-x

摘要： mutations have been found in many esophageal squamous cell carcinoma (ESCC) clinical specimens and cell lines. We reasoned that functional inactivation of wild-type or the functional activation of mutant-type might exist in these specimens and cell lines. In this study, we identified the correlation between p53 functional activation and its genotype in five different ESCC cell lines. To examine the potential p53 activation in a certain ESCC cell line, DNA damage methods including X-ray exposure and cisplatin treatment were employed to treat cells. Further, the expression of p53 protein and four transcripts of well-known p53 target genes were investigated using Western blot and reverse transcription-polymerase chain reaction (RT-PCR) after cell exposure to DNA damage. The results showed that in KYSE 30 cell line with mutant and KYSE 150 with wild-type , p53 could be activated by DNA damages. However, p53 could not be activated following the DNA damages in YES 2 with wild-type , KYSE 70 with mutant , and EC9706 with unknown genotype. All our data indicated that p53 function in certain cells is not closely correlated with its genotype. To judge p53 function in a particular cell line, it is important to examine the p53 functional activation, but not to simply rely on the genotype.

关键词： p53     esophageal squamous cell carcinoma     DNA damage    

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Heterogeneity of chronic obstructive pulmonary disease: from phenotype to genotype

null

《医学前沿（英文）》 2013年 第7卷 第4期   页码 425-432 doi: 10.1007/s11684-013-0295-x

摘要：

Chronic obstructive pulmonary disease (COPD) is one of the leading causes of morbidity and mortality throughout the world and is mainly characterized by persistent airflow limitation. Given that multiple systems other than the lung can be impaired in COPD patients, the traditional FEV1/FVC ratio shows many limitations in COPD diagnosis and assessment. Certain heterogeneities are found in terms of clinical manifestations, physiology, imaging findings, and inflammatory reactions in COPD patients; thus, phenotyping can provide effective information for the prognosis and treatment. However, phenotypes are often based on symptoms or pathophysiological impairments in late-stage COPD, and the role of phenotypes in COPD prevention and early diagnosis remains unclear. This shortcoming may be overcome by the potential genotypes defined by the heterogeneities in certain genes. This review briefly describes the heterogeneity of COPD, with focus on recent advances in the correlations between genotypes and phenotypes. The potential roles of these genotypes and phenotypes in the molecular mechanisms and management of COPD are also elucidated.

关键词： chronic obstructive pulmonary disease     heterogeneity     phenotype     genotype     prediction    

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Associations between

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《医学前沿（英文）》 2018年 第12卷 第1期   页码 92-97 doi: 10.1007/s11684-017-0610-z

摘要：

Investigations on the genetic diversity of Mycobacterium tuberculosis in China have shown that Beijing genotype strains play a dominant role. To study the association between the M.?tuberculosis Beijing genotype and the drug-resistance phenotype, 1286 M. tuberculosis clinical isolates together with epidemiological and clinical information of patients were collected from the center for tuberculosis (TB) prevention and control or TB hospitals in Beijing municipality and nine provinces or autonomous regions in China. Drug resistance testing was conducted on all the isolates to the four first-line anti-TB drugs (isoniazid, rifampicin, streptomycin, and ethambutol). A total of 585 strains were found to be resistant to at least one of the four anti-TB drugs. The Beijing family strains consisted of 499 (53.20%) drug-sensitive strains and 439 (46.80%) drug-resistant strains, whereas the non-Beijing family strains comprised 202 (58.05%) drug-sensitive strains and 146 (41.95%) drug-resistant strains. No significant difference was observed in prevalence (c2=2.41, P>0.05) between the drug-resistant and drug-sensitive strains among the Beijing family strains. Analysis of monoresistance, multidrug-resistant TB, and geographic distribution of drug resistance did not find any relationships between the M.?tuberculosis Beijing genotype and drug-resistance phenotype in China. Results confirmed that the Beijing genotype, the predominant M. tuberculosis genotype in China, was not associated with drug resistance.

关键词： tuberculosis     drug resistance     genotype     molecular biology    

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The epidemiology of norovirus gastroenteritis in China: disease burden and distribution of genotypes

Honglu Zhou, Songmei Wang, Lorenz von Seidlein, Xuanyi Wang

《医学前沿（英文）》 2020年 第14卷 第1期   页码 1-7 doi: 10.1007/s11684-019-0733-5

摘要： With the improvements of sanitation and nationwide safe water supply the occurrence of bacterial diarrhea declined remarkably, while viruses became the leading causes of acute gastroenteritis (AGE). Of these viruses, noroviruses (NoVs) are responsible for a considerable burden of gastroenteritis, especially in children<2 years and elderly≥65 years. NoVs circulating in the Chinese population are antigenically highly diverse with the genotype GII.4 being the dominant strain followed by GII.3. Given the widespread contamination in environmental sources, and highly infectious nature of NoVs, vaccination would be the desirable strategy for the control of NoV infections. However, a better understanding of acquired immunity after infection, and a reliable immunological surrogate marker are urgently needed, since two vaccine candidates based on virus-like particles (VLPs) are currently moving into clinical evaluations in China.

关键词： molecular epidemiology     norovirus     disease burden     genotype     China    

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Cyclooxygenase-2 gene-1195G/A genotype is associated with the risk of HBV-induced HCC: A case-control

Li-Feng LIU MD, PhD, Qiong CHEN MD, PhD, Ying CHANG MD, PhD, Ju-Sheng LIN MD, PhD, Jin-Liang ZHANG MM,

《医学前沿（英文）》 2010年 第4卷 第1期   页码 90-95 doi: 10.1007/s11684-010-0021-x

摘要： This study aimed to identify functional single nucleotide polymorphisms in the cyclooxygenase-2 gene promoter and evaluate their effects on the risk of primary hepatocellular carcinoma (HCC) with hepatitis B virus (HBV) infection. We conducted a population-based, case-control study enrolling 630 Han Chinese people in Hubei province. Subjects included primary HCC patients with HBV infection (=210), chronic hepatitis B cases (=210) and healthy Han Chinese (=210). -1195G/A polymorphism was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing analysis. We found-1195A allele carriers had a higher risk of HCC with HBV infection (OR, 0.72; 95% CI, 0.548–0.946). The-1195A allele might be used as a marker in screening individuals at high risk of HCC with HBV infection.

关键词： cyclooxygenase-2 gene     single nucleotide polymorphisms     susceptibility     primary hepatocellular carcinoma     hepatitis B virus infection    

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Advances in crop phenotyping and multi-environment trials

Zhe LIU,Fan ZHANG,Qin MA,Dong AN,Lin LI,Xiaodong ZHANG,Dehai ZHU,Shaoming LI

《农业科学与工程前沿（英文）》 2015年 第2卷 第1期   页码 28-37 doi: 10.15302/J-FASE-2015051

摘要： Efficient evaluation of crop phenotypes is a prerequisite for breeding, cultivar adoption, genomics and phenomics study. Plant genotyping is developing rapidly through the use of high-throughput sequencing techniques, while plant phenotyping has lagged far behind and it has become the rate-limiting factor in genetics, large-scale breeding and development of new cultivars. In this paper, we consider crop phenotyping technology under three categories. The first is high-throughput phenotyping techniques in controlled environments such as greenhouses or specifically designed platforms. The second is a phenotypic strengthening test in semi-controlled environments, especially for traits that are difficult to be tested in multi-environment trials (MET), such as lodging, drought and disease resistance. The third is MET in uncontrolled environments, in which crop plants are managed according to farmer’s cultural practices. Research and application of these phenotyping techniques are reviewed and methods for MET improvement proposed.

关键词： crop breeding     genotyping     phenotyping     genotype-environment interaction     cultivar regional test    

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The molecular epidemiological study of colistin-only-sensitive strains in multi-drug resistant Acinetobacter baumannii

YANG Li, HAN Lizhong, SUN Jingyong, NI Yuxing, YU Yunsong

《医学前沿（英文）》 2007年 第1卷 第4期   页码 423-428 doi: 10.1007/s11684-007-0083-6

摘要： This paper reported the epidemiology of the colistin-only-sensitive (COS-AB) in a tertiary teaching hospital in China. We analyzed the clinical data of 136 COS-AB isolates from June 2004 to May 2005 and collected 66 . isolates in which 33 strains were COS-AB, and the rest were non-COS-AB. Random amplified polymorphic DNA (RAPD) analysis (primer ERIC2 and 272) showed that all COS-AB were identical, while pulsed-field gel electrophotesis (PFGE) analysis showed two separate genotypes of these COS-AB which were distinctly different from that of non-COS-AB. The COS-AB from burn wards showed the identical PFGE pattern which was distinguished from the genotype of COS-AB in other departments, mainly surgical systems. The cross-infection was severe and strict methods of disinfection and sterilization should be implemented. Meanwhile, the epidemiology of COS-AB in environment and patients should be closely monitored. The PFGE analysis is a reliable method of . typing.

关键词： identical     different     teaching hospital     genotype     colistin-only-sensitive    

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Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity

AN Ke, XU Ying, TIAN Xuebi, GAO Feng, TIAN Yuke, YANG Hui, ZHANG Chuanhan

《医学前沿（英文）》 2008年 第2卷 第1期   页码 63-69 doi: 10.1007/s11684-008-0012-3

摘要： Neural progenitor cells (NPC) are those that are the source of neural cells for cell transplantation and gene therapy. The shortage in quantity and the limited life spans of primary cultured cells limit its widespread use in basic research. Immortalized NPC, which also possess the capacity of self-renewal and can proliferate infinitely, can produce a large number of NPCs with stable phenotype and genotype. Here we report that an immortalized neural progenitor cell strain, which we named as INPC, was successfully established by gene-transfer of simian virus 40 large T antigen gene mediated by liposomes. The INPC retained the biological characteristics of its original cells and provided a safe and reliable cell platform for the treatment of central nervous system diseases and transgenic cell transplantation. INPC could express low levels of MHC antigens which was down-regulated after differentiation. This indicates that INPC possesses poor immunogenicity. The immortalized cells may show good long-term survival and do not elicit an acute immunological response following transplantation.

关键词： immortalized     long-term survival     differentiation     capacity     genotype    

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Identification of pakchoi cultivars with low cadmium accumulation and soil factors that affect their cadmium uptake and translocation

Lin WANG,Yingming XU,Yuebing SUN,Xuefeng LIANG,Dasong LIN

《环境科学与工程前沿（英文）》 2014年 第8卷 第6期   页码 877-887 doi: 10.1007/s11783-014-0676-7

摘要： The selection and use of low-Cd-accumulating cultivar (LCAC) has been proposed as one of the promising approaches in minimizing the entry of Cd in the human food chain. This study suggests a screening criterion of LCACs focusing on food safety. Pot culture and plot experiments were conducted to screen out LCACs from 35 pakchoi cultivars and to identify the crucial soil factors that affect Cd accumulation in LCACs. Results of the pot culture experiment showed that shoot Cd concentrations under the three Cd treatments significantly varied across cultivars. Two cultivars, Hualv 2 and Huajun 2, were identified as LCACs because their shoot Cd concentrations were lower than 0.2 mg·kg under low Cd treatment and high Cd exposure did not affect the biomass of their shoots. The plot experiment further confirmed the consistency and genotypic stability of the low-Cd-accumulating traits of the two LCACs under various soil conditions. Results also showed that soil phosphorus availability was the most important soil factor in the Cd accumulation of pakchoi, which related negatively not only to Cd uptake by root but also to Cd translocation from root to shoot. The total Cd accumulation and translocation rates were lower in the LCACs than in the high-Cd cultivar, suggesting that Cd accumulation in different cultivars is associated with the Cd uptake by root as well as translocation from root to shoot. This study proves the feasibility of the application of the LCAC strategy in pakchoi cultivation to cope with Cd contamination in agricultural soils.

关键词： pakchoi (Brassica chinensis L.)     cadmium contamination     low-cadmium-accumulating cultivar (LCAC)     genotype variation     soil factor     translocation    

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不同类型意大利蜜蜂MDH Ⅱ同工酶的研究

陈盛禄,鲍秀良,苏松坤,刘艳荷

《中国工程科学》 2000年 第2卷 第1期   页码 57-61

摘要：

运用聚丙烯酰胺凝胶等电聚焦技术（IEF-PAGE），对有代表性的王浆蜂蜜双高产的"浙农大1号"意蜂品种（浙意，Ea）和中国本地意蜂（本意，Eb）、美国意蜂（美意，Em）、澳大利亚意蜂（澳意Eo）、意大利意蜂（原意，Ee）等5个意大利蜂（Apis mellifera Ligustica）品种试飞前的幼龄工蜂进行了苹果酸脱氢酶（MDH）同工酶的基因型、基因型频率、基因频率、杂合度和纯合度的研究。由a、b、c 3个等位基因编码的MDH图谱第二区带应有的a/a、a/b、a/c、b/b、b/c和c/c 6种基因型，在Ea、Eb、Ee 3种意蜂中全部出现；在Eo中出现a/b，a/c，b/c，c/c 4种，在Em中出现a/c，b/c，c/c 3种。联列表独立性检验结果，5个意蜂品种间的基因型频率、基因频率、杂合度、纯合度存在显著差异。

关键词： 意蜂品种     苹果酸脱氢酶     基因型     基因频率     杂合度    

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Association of novel mutations and heplotypes in the preS region of hepatitis B virus with hepatocellular carcinoma

Jia-Xin XIE, Jian-Hua YIN, Qi ZHANG, Rui PU, Wen-Ying LU, Hong-Wei ZHANG, Guang-Wen CAO, Jun ZHAO, Hong-Yang WANG,

《医学前沿（英文）》 2010年 第4卷 第4期   页码 419-429 doi: 10.1007/s11684-010-0160-0

摘要： The association of viral mutations and haplotypic carriages with mutations in the preS region of hepatitis B virus (HBV) genotypes B and C with hepatocellular carcinoma (HCC) is of great significance for the prediction of this malignancy, but it remains obscure. We analyzed the preS sequences of HBV genotypes B and C from 1172 HBV-infected subjects including 231 patients with HCC. As compared with the HBV-infected subjects without HCC, C2875T, G2946C, A3054C, C3060A, T3066C, C3116T, A3120C, G3191A, A1C, C7A, C10A, A31C, C76T, G105C, and G147C in both genotypes were significantly associated with increased risks of HCC. C2875A, G2950A, G2951A, A3054T, C3060T, T3066A, T3069G, A3120T, and G3191C were significantly associated with increased risks of HCC in genotype C, whereas these mutations were inversely associated with HCC in genotype B. Multivariate regression analyses showed that C76A/T was a novel factor independently associated with an increased risk of HCC, as compared with those without HCC. The frequencies of haplotypes 2964A-3116T-preS2 start codon wild-type-7C, 2964C-3116T-7A-76C, and 2964A-3116T-7C-76A/T were significantly higher in the patients with HCC (<0.001), whereas a haplotypic carriage with a single mutation and another three wild-types were inversely associated with HCC. Conclusively, the association of HBV mutations in the preS region with HCC depends on HBV genotype and haplotypic carriage with two or more mutations that are each associated with an increased risk of HCC independently.

关键词： hepatitis B virus     hepatocellular carcinoma     mutation     genotype     haplotype    

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标题 作者 时间 类型 操作

Genotype variations in accumulation of cadmium and lead in celery (

Kun ZHANG, Jianbing WANG, Zhongyi YANG, Guorong XIN, Jiangang YUAN, Junliang XIN, Charlie HUANG

期刊论文

p53 functional activation is independent of its genotype in five esophageal squamous cell carcinoma cell

Junfang JI, Kun WU, Min WU, Qimin ZHAN,

期刊论文

Heterogeneity of chronic obstructive pulmonary disease: from phenotype to genotype

null

期刊论文

Associations between

null

期刊论文

The epidemiology of norovirus gastroenteritis in China: disease burden and distribution of genotypes

Honglu Zhou, Songmei Wang, Lorenz von Seidlein, Xuanyi Wang

期刊论文

Cyclooxygenase-2 gene-1195G/A genotype is associated with the risk of HBV-induced HCC: A case-control

Li-Feng LIU MD, PhD, Qiong CHEN MD, PhD, Ying CHANG MD, PhD, Ju-Sheng LIN MD, PhD, Jin-Liang ZHANG MM,

期刊论文

Advances in crop phenotyping and multi-environment trials

Zhe LIU,Fan ZHANG,Qin MA,Dong AN,Lin LI,Xiaodong ZHANG,Dehai ZHU,Shaoming LI

期刊论文

The molecular epidemiological study of colistin-only-sensitive strains in multi-drug resistant Acinetobacter baumannii

YANG Li, HAN Lizhong, SUN Jingyong, NI Yuxing, YU Yunsong

期刊论文

Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity

AN Ke, XU Ying, TIAN Xuebi, GAO Feng, TIAN Yuke, YANG Hui, ZHANG Chuanhan

期刊论文

Identification of pakchoi cultivars with low cadmium accumulation and soil factors that affect their cadmium uptake and translocation

Lin WANG,Yingming XU,Yuebing SUN,Xuefeng LIANG,Dasong LIN

期刊论文

不同类型意大利蜜蜂MDH Ⅱ同工酶的研究

陈盛禄,鲍秀良,苏松坤,刘艳荷

期刊论文

Association of novel mutations and heplotypes in the preS region of hepatitis B virus with hepatocellular carcinoma

Jia-Xin XIE, Jian-Hua YIN, Qi ZHANG, Rui PU, Wen-Ying LU, Hong-Wei ZHANG, Guang-Wen CAO, Jun ZHAO, Hong-Yang WANG,

期刊论文