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期刊论文 1

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2008 1

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Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor

SONG Kedong, LIU Tianqing, ZHAO Hu, LI Xiangqin, MA Xuehu, CUI Zhanfeng

《化学科学与工程前沿(英文)》 2008年 第2卷 第1期   页码 34-39 doi: 10.1007/s11705-008-0022-3

摘要: Large-scale expansion of the osteoblasts of a Sprague-Dawley (SD) rat was studied in a rotating wall hollow-fiber membrane bioreactor (RWHMB) by using hollow-fiber membrane as the carrier. For the sake of contrast, cells were also expanded in a T-flask using a hollow-fiber membrane as carrier and in a rotating wall vessel bioreactor (RWVB) using a microcarrier. During the culture period, the cells were sampled every 12 h, and after 5 days, the cells were harvested and evaluated with scanning electron microscopy (SEM), hematoxylin-eosin (HE) staining and alkaline phosphatase (ALP) staining. Moreover, von-Kossa staining and Alizarin Red S staining were carried out for mineralized nodules formation. The results show that in RWHMB, the cells present better morphology and vitality and secrete much more extracellular matrix. It is concluded that the RWHMB combines the advantages of the rotating wall vessel and hollow-fiber membrane bioreactors. The hydrodynamic stimulation within it accelerates the metabolism of the osteoblast and mass transfer, which is propitious to cell differentiation and proliferation.

关键词: RWHMB     microcarrier     hematoxylin-eosin     von-Kossa staining     propitious    

标题 作者 时间 类型 操作

Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor

SONG Kedong, LIU Tianqing, ZHAO Hu, LI Xiangqin, MA Xuehu, CUI Zhanfeng

期刊论文