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Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed humanmetaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

《医学前沿(英文)》 2021年 第15卷 第1期   页码 144-154 doi: 10.1007/s11684-020-0792-7

摘要: Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30–32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.

关键词: human metaphase II oocyte     vitrification     cryostorage duration     single-cell RNA-Seq     lncRNA    

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

《农业科学与工程前沿(英文)》 2014年 第1卷 第3期   页码 185-190 doi: 10.15302/J-FASE-2014035

摘要: DNA damage is one of the most common threats to meiotic cells. It has the potential to induce infertility and genetic abnormalities that may be passed to the embryo. Here, we reviewed exogenous factors which could induce DNA damage. Specially, we addressed the different effects of DNA damage on mouse oocytes and embryonic development. Complex DNA damage, double-strand breaks, represents a more difficult repair process and involves various repair pathways. Understanding the mechanisms involved in DNA damage responses may improve therapeutic strategies for ovarian cancer and fertility preservation.

关键词: DNA damage     double-strand breaks (DSBs)     oocyte     embryo    

HLA and lung transplantation

Liya Ju, Caroline Suberbielle, Xiaofan Li, Nuala Mooney, Dominique Charron

《医学前沿(英文)》 2019年 第13卷 第3期   页码 298-313 doi: 10.1007/s11684-018-0636-x

摘要: Lung transplantation is increasingly practiced for patients with end-stage lung disease. The successful outcome of solid organ transplantation today is severely impeded by the production of alloantibodies, mainly directed against the protein products of the HLA complex of the organ donor. While the association between antibody mediated rejection and allograft damage has been well established in renal and heart transplantation, it has not yet been well characterized in lung transplantation. This review addresses the question of HLA matching in lung transplantation and current knowledge of the allogenicity of different HLA class I and II antigens. The role of the antibody mediated immune response is discussed as well as the importance of pre-transplant or post-transplant circulating antibodies. Finally, potential mechanisms, which may act individually or in combination, of antibody mediated damage to solid organ transplants are considered.

关键词: human leukocyte antigen class I and II     lung transplantation     mismatch     obliterans bronchiolitis     alloantibody     antibody mediated rejection    

表面张力辅助去核法对小鼠卵母细胞的去核研究

孟庆刚,朱士恩,曾申明,张忠诚

《中国工程科学》 2001年 第3卷 第11期   页码 65-69

摘要:

论述了一种新的小鼠卵母细胞去核方法一一表面张力辅助去核法(STA),并同时与其他3种去核方 法(简称A法、B法、C法)进行比较。STA法是将卵母细胞核在固定管的辅助下,借助液体表面张力通过透 明带上的破口被挤出。经STA法去核的卵母细胞质体用于电融合介导的小鼠耳成纤维细胞核移植实验,结果有 76.1 %细胞与细胞质体融合形成重组胚,85.4 %的重组胚形成原核,49.4%卵裂形成2-细胞。

关键词: 表面张力辅助     去核     卵母细胞     小鼠    

Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

《医学前沿(英文)》 2018年 第12卷 第5期   页码 518-524 doi: 10.1007/s11684-017-0575-y

摘要:

The increased levels of intracellular reactive oxygen species (ROS) in granulosa cells (GCs) may affect the pregnancy results in women with polycystic ovary syndrome (PCOS). In this study, we compared thein vitro fertilization and embryo transfer (IVF-ET) results of 22 patients with PCOS and 25 patients with tubal factor infertility and detected the ROS levels in the GCs of these two groups. Results showed that the PCOS group had significantly larger follicles on the administration day for human chorionic gonadotropin than the tubal factor group (P<0.05); however, the number of retrieved oocytes was not significantly different between the two groups (P>0.05). PCOS group had slightly lower fertilization, cleavage, grade I/II embryo, clinical pregnancy, and implantation rates and higher miscarriage rate than the tubal factor group (P>0.05). We further found a significantly higher ROS level of GCs in the PCOS group than in the tubal factor group (P<0.05). The increased ROS levels in GCs caused GC apoptosis, whereas NADPH oxidase 2 (NOX2) specific inhibitors (diphenyleneiodonium and apocynin) significantly reduced the ROS production in the PCOS group. In conclusion, the increased ROS expression levels in PCOS GCs greatly induced cell apoptosis, which further affected the oocyte quality and reduced the positive IVF-ET pregnancy results of women with PCOS. NADPH oxidase pathway may be involved in the mechanism of ROS production in GCs of women with PCOS.

关键词: PCOS     ROS     granulosa cell     IVF-ET     NADPH oxidase    

electrolysis cells with biocathodes and driven by microbial fuel cells for simultaneous enhanced Co(II) and Cu(II) removal

Jingya SHEN,Yuliang SUN,Liping HUANG,Jinhui YANG

《环境科学与工程前沿(英文)》 2015年 第9卷 第6期   页码 1084-1095 doi: 10.1007/s11783-015-0805-y

摘要: Cobalt and copper recovery from aqueous Co(II) and Cu(II) is one critical step for cobalt and copper wastewaters treatment. Previous tests have primarily examined Cu(II) and Co(II) removal in microbial electrolysis cells (MECs) with abiotic cathodes and driven by microbial fuel cell (MFCs). However, Cu(II) and Co(II) removal rates were still slow. Here we report MECs with biocathodes and driven by MFCs where enhanced removal rates of 6.0±0.2 mg?L ?h for Cu(II) at an initial concentration of 50 mg?L and 5.3±0.4 mg?L h for Co(II) at an initial 40 mg?L were achieved, 1.7 times and 3.3 times as high as those in MECs with abiotic cathodes and driven by MFCs. Species of Cu(II) was reduced to pure copper on the cathodes of MFCs whereas Co(II) was removed associated with microorganisms on the cathodes of the connected MECs. Higher Cu(II) concentrations and smaller working volumes in the cathode chambers of MFCs further improved removal rates of Cu(II) (115.7 mg?L ?h ) and Co(II) (6.4 mg?L ?h ) with concomitantly achieving hydrogen generation (0.05±0.00 mol?mol COD). Phylogenetic analysis on the biocathodes indicates dominantly accounted for 67.9% of the total reads, followed by (14.0%), (6.1%), (2.5%), (1.4%), and (1.0%). This study provides a beneficial attempt to achieve simultaneous enhanced Cu(II) and Co(II) removal, and efficient Cu(II) and Co(II) wastewaters treatment without any external energy consumption.

关键词: biocathode     microbial electrolysis cell     microbial fuel cell     Cu(II) removal     Co(II) removal    

A microextraction approach for rapid extraction and separation of Mn(II) and Co(II) using saponified

《化学科学与工程前沿(英文)》 2022年 第16卷 第6期   页码 963-972 doi: 10.1007/s11705-021-2081-7

摘要: In this paper, we proposed a microextraction approach for the extraction and separation of Mn(II) and Co(II) from sulfate solution simulating leachate of spent lithium-ion battery cathode materials using saponified di-(2-ethylhexyl)phosphoric acid system. The effects of the following operational variables were investigated: equilibrium pH, tri-n-butyl phosphate concentration, saponification rate, two-phase ratio and residence time. The results showcased that the microextractor can reach the extraction equilibrium within 20 s, thereby greatly reducing necessary extraction time comparing to that of conventional processes. The volumetric mass transfer coefficient showed 8–21 times larger than that of batch device. With the help of microextractor, 95% of Mn(II) was extracted with a single theoretical stage at a chosen two-phase ratio of 3:1, and the separation factor βMn/Co was as large as 65.5. In the subsequent stripping step, more than 99% of manganese from loaded phase was easily stripped under optimal conditions. The microextraction approach greatly enhances the mass transfer while enabling a continuous and controllable extraction process within a simple structure design. When extracting spent electrode material with microextractors, the comprehensive recovery of mangenese can reach 96%. The microextraction approach has a good applicability in the spent lithium-ion battery cathode materials recycling at both bench and industrial scales.

关键词: extraction equilibrium     mass transfer coefficient     microextraction     multicomponent extraction     di-(2-ethylhexyl)phosphoric acid    

Co-present Pb(II) accelerates the oxidation of organic contaminants by permanganate: Role of Pb(III)

《环境科学与工程前沿(英文)》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1530-y

摘要:

• Simultaneous removal of organic contaminants and Pb(II) was achieved by Mn(VII).

关键词: Permanganate     Pb(II) oxidation     MnO2     pH effect    

Removal of Ni(II) ions from wastewater by micellar enhanced ultrafiltration using mixed surfactants

Amar D. Vibhandik, Kumudini V. Marathe

《化学科学与工程前沿(英文)》 2014年 第8卷 第1期   页码 79-86 doi: 10.1007/s11705-014-1407-0

摘要: Ni(II) ions were removed from aqueous waste using micellar enhanced ultrafiltration (MEUF) with a mixture of surfactants. The surfactant mixture was the nonionic surfactant Tween 80 (TW80) mixed with the anionic surfactant sodium dodecyl sulfate (SDS) in different molar ratios ranging from 0.1–1.5. The operational variables of the MEUF process such as pH, applied pressure, surfactant to metal ion ratio and nonionic to ionic surfactant molar ratio (α) were evaluated. Rejection of Ni and TW80 was 99% and 98% respectively whereas that for SDS was 65%. The flux and all resistances (fouling resistance, resistance due to concentration polarization) were measured and calculated for entire range of α respectively. A calculated flux was found to be declined with time, which was mainly attributed to concentration polarization rather than resistance from membrane fouling.

关键词: MEUF     Ni (II) ions     membrane resistance     concentration polarization     mixed surfactants    

Multi-objective optimization of a hybrid distributed energy system using NSGA-II algorithm

Hongbo REN, Yinlong LU, Qiong WU, Xiu YANG, Aolin ZHOU

《能源前沿(英文)》 2018年 第12卷 第4期   页码 518-528 doi: 10.1007/s11708-018-0594-7

摘要:

In this paper, a multi-objective optimization model is established for the investment plan and operation management of a hybrid distributed energy system. Considering both economic and environmental benefits, the overall annual cost and emissions of CO2 equivalents are selected as the objective functions to be minimized. In addition, relevant constraints are included to guarantee that the optimized system is reliable to satisfy the energy demands. To solve the optimization model, the non-dominated sorting generic algorithm II (NSGA-II) is employed to derive a set of non-dominated Pareto solutions. The diversity of Pareto solutions is conserved by a crowding distance operator, and the best compromised Pareto solution is determined based on the fuzzy set theory. As an illustrative example, a hotel building is selected for study to verify the effectiveness of the optimization model and the solving algorithm. The results obtained from the numerical study indicate that the NSGA-II results in more diversified Pareto solutions and the fuzzy set theory picks out a better combination of device capacities with reasonable operating strategies.

关键词: multi-objective optimization     hybrid distributed energy system     non-dominated sorting generic algorithm II     fuzzy set theory     Pareto optimal solution    

Microfluidic synthesis of renewable biosorbent with highly comprehensive adsorption performance for copper (II

Yong Zhu, Zhishan Bai, Bingjie Wang, Linlin Zhai, Wenqiang Luo

《化学科学与工程前沿(英文)》 2017年 第11卷 第2期   页码 238-251 doi: 10.1007/s11705-017-1627-1

摘要: A microsphere biosorbent with uniform size (CV= 1.52%), controllable morphology and component, and high mechanical strength was synthesized from chitosan by microfluidic technology combining with chemical crosslinking and solvent extraction. This chitosan microsphere (CS-MS) was prepared with a two-step solidification process, which was acquired by drying for the enhancement of mechanical property in final. The adsorption behavior of CS-MS towards copper (II) and main influencing factors on adsorption performance were investigated by batch experiments. Kinetic data highlighted dominant chemical bonding along with electrons transferring in adsorption process. Isothermal analysis indicated that adsorption capacity was relevant to the number of active site. All these explorations provided a new direction for preparing highly comprehensive performance sorbent used in heavy metal treatment via microfluidic technology.

关键词: chitosan microsphere     microfluidic technology     adsorption     copper (II)    

Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF

Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG

《环境科学与工程前沿(英文)》 2016年 第10卷 第2期   页码 362-367 doi: 10.1007/s11783-015-0803-0

摘要: Interactions between metals and activated sludge can substantially affect the fate and transport of heavy metals in wastewater treatment plants. Therefore, it is important to develop a simple, fast and efficient method to elucidate the interaction. In this study, a modified titration method with a dynamic mode was developed to investigate the binding of Cu(II), a typical heavy metal, onto aerobic granules. The titration results indicated that pH and ionic strength both had a positive effect on the biosorption capacity of the granular sludge. The -XRF results demonstrated that the distribution of metals on the granular surface was heterogeneous, and Cu showed strong correlations and had the same “hot spots” positions with other metal ions (e.g., Ca, Mg, Fe etc.). Ion exchange and complexing were the main mechanisms for the biosorption of Cu(II) by aerobic granules. These results would be beneficial for better understanding of Cu(II) migration and its fate in wastewater treatment plants.

关键词: aerobic granules     Cu(II)     modified titration     µ-XRF analysis    

The performance of nitrate-reducing Fe(II) oxidation processes under variable initial Fe/N ratios: The

《环境科学与工程前沿(英文)》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1366-2

摘要:

•Bacterially-mediated coupled N and Fe processes examined in incubation experiments.

关键词: Denitrification     N2O emission     Fe(II) oxidation     Fe/N ratio     Fe minerals    

Efficient removal of Cr(VI) and Pb(II) from aqueous solution by magnetic nitrogen-doped carbon

Wanyue Liu, Xiaoqin Liu, Jinming Chang, Feng Jiang, Shishi Pang, Hejun Gao, Yunwen Liao, Sheng Yu

《化学科学与工程前沿(英文)》 2021年 第15卷 第5期   页码 1185-1196 doi: 10.1007/s11705-020-2032-8

摘要: The magnetic nitrogen-doped carbon (MNC) was prepared from polypyrrole by a simple high temperature calcination process in this paper. The structure and properties of MNC were analyzed by scanning electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction, Brunner Emmet Teller, vibrating sample magnetometer, and X-ray photoelectron spectroscopy. The capacity of MNC to adsorb Cr(VI) and Pb(II) was evaluated. The effects of the initial pH, dosage, concentration and temperature on the adsorption capacity of MNC were measured. MNC had a large specific surface area and a special porous structure. Its nitrogen and carbon sources were rich, and the ratio of carbon to nitrogen was fixed. The maximum Cr(VI)-adsorption capacity and maximum Pb(II) adsorption capacity of MNC could reach 456.63 and 507.13 mg∙g at 318 K, respectively. The pseudo-second-order model was used to describe the adsorption kinetics of MNC, and the Freundlich model was employed to discuss its isotherms. The adsorption process was affected by the electrostatic force, the reducing reaction, pores and chelation. The results of this study suggest that MNC is a material with superior performance, and is very easily regenerated, reused, and separated in the adsorption process.

关键词: magnetic     nitrogen-doped carbon     adsorption     Cr(VI)     Pb(II)    

Optimization of microwave-assisted extraction for picroside I and picroside II from

Pravin WAKTE,Ajit PATIL,Bhusari SACHIN,Munnaza QUAZI,Shraddha JABDE,Devanand SHINDE

《化学科学与工程前沿(英文)》 2014年 第8卷 第4期   页码 445-453 doi: 10.1007/s11705-014-1458-2

摘要: The response surface methodology was employed to study the optimization of microwave-assisted extraction of picroside I and picroside II from Royle rhizomes. The effects of solid to solvent ratio, and extraction temperature, time and solvent on the yields of picroside I and picroside II have been investigated using Box-Behnken experimental design. The experimental data were fitted to second-order polynomial equations using multiple regression analysis and analyzed using the appropriate statistical method. By solving the regression equation and analyzing 3-D plots, the optimum extraction conditions were found to be: solid to solvent ratio, 10 : 90 (w/v); temperature, 60 °C; and extraction time, 60 s. Under the optimal conditions, the yields of picroside I and picroside II are 41.23 and 6.12 mg·g feed respectively, which are in good agreement with the predicted values. The ratio of solid to solvent significantly affects the yields of picroside I and picroside II. Application of microwave-assisted extraction of picroside I and picroside II from would dramatically reduce extraction time and solvent consumption.

关键词: Box-Behnken design     microwave-assisted extraction     picroside I     picroside II     Picrorrhiza kurroa    

标题 作者 时间 类型 操作

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed humanmetaphase II oocytes

Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao

期刊论文

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

期刊论文

HLA and lung transplantation

Liya Ju, Caroline Suberbielle, Xiaofan Li, Nuala Mooney, Dominique Charron

期刊论文

表面张力辅助去核法对小鼠卵母细胞的去核研究

孟庆刚,朱士恩,曾申明,张忠诚

期刊论文

Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with

Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin

期刊论文

electrolysis cells with biocathodes and driven by microbial fuel cells for simultaneous enhanced Co(II) and Cu(II) removal

Jingya SHEN,Yuliang SUN,Liping HUANG,Jinhui YANG

期刊论文

A microextraction approach for rapid extraction and separation of Mn(II) and Co(II) using saponified

期刊论文

Co-present Pb(II) accelerates the oxidation of organic contaminants by permanganate: Role of Pb(III)

期刊论文

Removal of Ni(II) ions from wastewater by micellar enhanced ultrafiltration using mixed surfactants

Amar D. Vibhandik, Kumudini V. Marathe

期刊论文

Multi-objective optimization of a hybrid distributed energy system using NSGA-II algorithm

Hongbo REN, Yinlong LU, Qiong WU, Xiu YANG, Aolin ZHOU

期刊论文

Microfluidic synthesis of renewable biosorbent with highly comprehensive adsorption performance for copper (II

Yong Zhu, Zhishan Bai, Bingjie Wang, Linlin Zhai, Wenqiang Luo

期刊论文

Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF

Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG

期刊论文

The performance of nitrate-reducing Fe(II) oxidation processes under variable initial Fe/N ratios: The

期刊论文

Efficient removal of Cr(VI) and Pb(II) from aqueous solution by magnetic nitrogen-doped carbon

Wanyue Liu, Xiaoqin Liu, Jinming Chang, Feng Jiang, Shishi Pang, Hejun Gao, Yunwen Liao, Sheng Yu

期刊论文

Optimization of microwave-assisted extraction for picroside I and picroside II from

Pravin WAKTE,Ajit PATIL,Bhusari SACHIN,Munnaza QUAZI,Shraddha JABDE,Devanand SHINDE

期刊论文