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期刊论文 8

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2015 1

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N-聚糖 1

ε-不敏感损失函数 1

亲水相互作用液相色谱法 1

例外点 1

全氟化碳 1

双调线圈 1

可逆标签 1

权重因子 1

毛细管凝胶电泳 1

1

1

磁共振成像(MRI) 1

糖蛋白 1

细胞标记 1

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血管生成术 1

质谱法 1

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PREFERENCE FOR ENVIRONMENTALLY-FRIENDLY BEVERAGE PACKAGING: THE ROLE OF INFORMATION PROVISION AND IDENTITY LABELING

《农业科学与工程前沿(英文)》 2023年 第10卷 第1期   页码 95-108 doi: 10.15302/J-FASE-2022478

摘要:

● Consumer preference for environmentally-friendly beverage packaging was investigated.

关键词: China     consumer preference     food and beverage packaging     green identity label     information treatment     plastics    

Decision Support System for Evaluating Impact of Product Carbon Labeling Scheme

Xu Dong-ling,Foster Chris,Hu Ying,Yang Jian-bo

《工程管理前沿(英文)》 2014年 第1卷 第1期   页码 89-104 doi: 10.15302/J-FEM-2014015

摘要: In this paper a decision support system for systematically evaluating the impact of labeling products with their carbon footprints is developed and applied to prioritize products for carbon labeling in a large supermarket chain in the UK. Carbon labels may change consumers' behavior and encourage suppliers to implement carbon-reduction solutions. Those changes may, however, lead to unintended risks. To handle the challenges of uncertainties in the evaluation, the Evidential Reasoning approach and the Intelligent Decision System software for multi-criteria decision analysis are applied to support the process. The system developed can be applied to assessing the impact of sustainable development policies to maximize their benefits and minimize their risks.

关键词: impact assessment     multiple criteria decision analysis     risk analysis     carbon footprint     decision support system     product carbon labeling    

Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression variation of VEGF

ZHAN Rong, YU Qinghong, HUANG Haobo

《医学前沿(英文)》 2008年 第2卷 第4期   页码 356-360 doi: 10.1007/s11684-008-0068-0

摘要: The aim of this article is to explore the effect of arsenic trioxide (AsO) on the proliferation and apoptosis of myeloma cell line U266 and its relationship with the expression variation of vascular endothelial growth factor (VEGF). The viability and apoptosis of U266 cells were observed by methylthiazolyl- tetrazolium (MTT) assay and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). The effect of AsO on the VEGF expression of U266 cells were tested by enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. We found that AsO could significantly inhibit the growth of U266 cells, and the concentration for 50% growth inhibition (IC) was 2 ?mol/L. After treatment with 2, 5, 10 ?mol/L AsO for 36 hours, dose-dependent apoptosis of U266 cells was observed. After treatment with 2, 5, 10 ?mol/L AsO for 72 hours, a dose-dependent reduction of VEGF in the supernatant of U266 cells culture was found. As far as single cells are concerned, nevertheless, the expression of VEGF mRNA did not vary. So we draw the conclusion that AsO could induce the apoptosis of U266 cells and inhibit their proliferation, decrease the tumor load, and lead to the reduction of VEGF in the culture supernatant, but not change the expression of VEGF in single U266 cells.

关键词: VEGF expression     expression variation     culture supernatant     labeling     concentration    

A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

《化学科学与工程前沿(英文)》 2022年 第16卷 第1期   页码 121-127 doi: 10.1007/s11705-021-2051-0

摘要: Lysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinity-based labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.

关键词: dual-function     fluorescent probe     labeling     photo-cross-linker     lipoylation modification     eraser     sirtuin    

Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

《工程(英文)》 doi: 10.1016/j.eng.2023.02.016

摘要: As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans’ immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach “visualizes” N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.

关键词: Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gel electrophoresis     Mass spectrometry    

可去除染料——N-聚糖多方法深入分析中的缺失环节 Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

《工程(英文)》 2023年 第26卷 第7期   页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

摘要:

As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans'  immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.

关键词: 糖蛋白     N-聚糖     可逆标签     亲水相互作用液相色谱法     毛细管凝胶电泳     质谱法    

全氟化碳乳剂19F磁共振成像的最新进展 Review

Anne H. Schmieder,Shelton D. Caruthers,Jochen Keupp,Samuel A. Wickline,Gregory M. Lanza

《工程(英文)》 2015年 第1卷 第4期   页码 475-489 doi: 10.15302/J-ENG-2015103

摘要:

19F磁共振成像(MRI) 的研究可追溯到30多年前。在这30多年间,氢原子核(1H)成像技术迅速发展,并在全球得到广泛应用,使得磁共振成像成为生物医学诊断成像技术中不可缺少的支柱。多年来,由于各种原因,对19F成像技术的研究进展缓慢。但是在过去的十年间,尤其是最近几年,19F成像的研究和临床相关性呈爆发式发展。部分原因归结为MRI仪器、19F/1H线圈设计以及临床前和临床核磁共振仪的超高速脉冲序列的发展。这些成就再加上对解剖生理学分子成像技术的兴趣以及一批创新造影剂的出现使19F的概念进入了早期的临床评估中。本篇综述重点探讨以液态全氟化碳化合物为基础的造影剂,并试图呈现这段丰富的研究和发展历史。

关键词:     磁共振成像(MRI)     双调线圈     全氟化碳     血管生成术     细胞标记    

鲁棒的极大熵聚类算法RMEC及其例外点标识

邓赵红,王士同,吴锡生,胡德文

《中国工程科学》 2004年 第6卷 第9期   页码 38-45

摘要:

针对极大熵聚类算法MEC(maximum entropy clustering)对例外点(outliers)较敏感和不能标识例外点的缺陷,提出了一种改进的极大熵聚类算法RMEC(robust maximum entropy clustering)。该算法的基本思想是通过引入Vapnik's ε-不敏感损失函数和权重因子重新构建目标函数,并利用优化理论推导出新的学习公式。RMEC算法不但对例外点较之MEC算法有更好的鲁棒性,而且还能有效地利用学习后的权重因子标识出数据集中存在的例外点。仿真试验结果亦表明了RMEC算法的上述优点。

关键词:     聚类     鲁棒性     例外点     ε-不敏感损失函数     权重因子    

标题 作者 时间 类型 操作

PREFERENCE FOR ENVIRONMENTALLY-FRIENDLY BEVERAGE PACKAGING: THE ROLE OF INFORMATION PROVISION AND IDENTITY LABELING

期刊论文

Decision Support System for Evaluating Impact of Product Carbon Labeling Scheme

Xu Dong-ling,Foster Chris,Hu Ying,Yang Jian-bo

期刊论文

Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression variation of VEGF

ZHAN Rong, YU Qinghong, HUANG Haobo

期刊论文

A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

期刊论文

Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches

Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,

期刊论文

可去除染料——N-聚糖多方法深入分析中的缺失环节

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

期刊论文

全氟化碳乳剂19F磁共振成像的最新进展

Anne H. Schmieder,Shelton D. Caruthers,Jochen Keupp,Samuel A. Wickline,Gregory M. Lanza

期刊论文

鲁棒的极大熵聚类算法RMEC及其例外点标识

邓赵红,王士同,吴锡生,胡德文

期刊论文