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FGF13 suppresses acute myeloid leukemia by regulating bone marrow niches
《医学前沿(英文)》 2022年 第16卷 第6期 页码 896-908 doi: 10.1007/s11684-022-0944-z
关键词: acute myeloid leukemia FGF13 prognosis immune-related genes bone marrow niches
Autologous bone marrow stem cell transplantation for the treatment of ulcerative colitis complicated
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《医学前沿(英文)》 2016年 第10卷 第4期 页码 522-526 doi: 10.1007/s11684-016-0485-4
Ulcerative colitis (UC) is a chronic inflammatory bowel disease with continuous or recurrent symptoms. A 42-year-old male patient with intermittent diarrhea accompanied by bloody mucopurulent stools was admitted to our hospital. The diagnosis of UC was confirmed by a combination of laboratory examination, colonoscopy, and histological assay. The patient developed herpes zoster in the hospital, which challenged traditional treatments. Therefore, we performed an autologous bone marrow cells to modulate the immune system with his permission. Autologous bone marrow mononuclear cells were collected and injected locally into the bowel mucosa, and subsequently injected systemically through a peripheral vein. After the patient underwent auto bone marrow mononuclear cells transplantations twice, the patient’s symptoms were alleviated. Furthermore, he recovered from hematochezia, and his hypersensitive C reactive protein decreased. Colonoscopy results showed reduced lesions and decreased areas with bleeding and edema in the sigmoid colon and rectum. No recurrence occurred in the subsequent two years, but long-time monitoring is still necessary for the prophylaxis of colorectal cancer.
关键词: autograft bone marrow stem cells ulcerative colitis cell therapy
《医学前沿(英文)》 2021年 第15卷 第5期 页码 718-727 doi: 10.1007/s11684-020-0807-4
关键词: severe aplastic anemia non-severe acquired aplastic anemia haploidentical bone marrow transplantation outcomes
李连达,吴理茂,刘红,宁可永,李贻奎
《中国工程科学》 2004年 第6卷 第8期 页码 1-4
细胞移植作为一种新的治疗方法备受医学界关注,但细胞供应短缺和免疫排斥是阻碍这一疗法临床推广的主要难题。自体骨髓干细胞移植在一定程度上可缓解此矛盾。文章综述了自体骨髓干细胞移植治疗脑、肝、心等重要器官疾病的基础和临床研究现状,以及骨髓动员剂在骨髓干细胞移植中的作用。
FANG Baijun, SONG Yongping, LIN Quande, ZHAO Chunhua, SHI Mingxia
《医学前沿(英文)》 2007年 第1卷 第2期 页码 185-191 doi: 10.1007/s11684-007-0035-1
关键词: endothelial phenomenon ectodermal Flk1CD34 postembryonic irradiated non-obese
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《医学前沿(英文)》 2016年 第10卷 第3期 页码 286-296 doi: 10.1007/s11684-016-0456-9
Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells (BMSCs) toward the injured area. This study aimed to determine the following: (1) the direction of rat BMSC (rBMSC) migration upon exposure to a direct current electric field (DCEF), (2) the optimal DCEF intensity and duration, and (3) the possible regulatory role of SDF-1/CXCR4 axis in rBMSC migration as induced by DCEF. Results showed that rBMSCs migrated to the positive electrode of the DCEF, and that the DCEF of 200 mV/mm for 4 h was found to be optimal in enhancing rBMSC migration. This DCEF strength and duration also upregulated the expression of osteoblastic genes, including ALP and OCN, and upregulated the expression of ALP and Runx2 proteins. Moreover, when CXCR4 was inhibited, rBMSC migration due to DCEF was partially blocked. These findings indicated that DCEF can effectively induce rBMSC migration. A DCEF of 200 mV/mm for 4 h was recommended because of its ability to promote rBMSC migration, proliferation, and osteogenic differentiation. The SDF-1/CXCR4 signaling pathway may play an important role in regulating the DCEF-induced migration of rBMSCs.
关键词: DCEF migration osteogenesis differentiation rBMSCs SDF-1/CXCR-4
张荣利,冯 雪,张会亮,罗国安,李连达,王义明
《中国工程科学》 2007年 第9卷 第7期 页码 89-94
采用密度梯度离心法分离骨髓间质干细胞,差速贴壁法进行纯化,应用流式细胞仪、激光共聚焦显微镜等对纯化细胞进行鉴定,结果细胞表面抗原CD29,CD44,CD105,CD166表达呈阳性,而CD14,CD34,CD45表达呈阴性。采用RT唱PCR鉴定了三个基因:nestin,NST,Oct-4,前两者呈阳性表达,后者弱阳性表达。这些细胞特异抗原与基因表达综合起来,表明得到的细胞具备骨髓间质干细胞的特性。密度梯度分离与差速贴壁相结合,可获得较好均一性的骨髓间质干细胞,是一种简单可靠、易于推广的骨髓干细胞获取方法,可为细胞与组织工程研究提供种子细胞。
吴理茂,李连达,刘红,宁可永,李贻奎
《中国工程科学》 2004年 第6卷 第7期 页码 34-42
研究中药归元方与自体骨髓干细胞移植对急慢性肝损伤的治疗作用。研究方法:用肝脏局部注射乙醇的方法复制急性局限性肝损伤模型,复合因素(CCl4、乙醇、高脂、低蛋白)刺激复制大鼠肝纤维化模型,通过定量组织学、肝功能检查、免疫组化、肝组织羟脯氨酸含量、损伤或纤维区骨髓干细胞观察等综合评价中药、自体骨髓干细胞移植及两者合用的疗效。结果:归元方与自体骨髓干细胞移植可减小肝损伤区域,改善肝功能,使纤维肝组织表达μPA增强,降低血清ALT,AST,PCⅢ,HA和肝组织羟脯氨酸的含量,改善肝组织肝纤维化评分,骨髓干细胞能在肝损伤、肝纤维化形成环境中存活、增殖,并向肝细胞分化,表达肝脏特异的角蛋白CK18。结论:归元方与自体骨髓干细胞移植对急慢性肝损伤有明确的治疗作用,两者合用可优势互补,协同增效。临床上有良好的应用前景。
Mechanisms of “kidney governing bones” theory in traditional Chinese medicine
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《医学前沿(英文)》 2014年 第8卷 第3期 页码 389-393 doi: 10.1007/s11684-014-0362-y
Studies conducted by our group on the mechanism of “kidney governing bones” theory in traditional Chinese medicine (TCM) are reviewed in this paper. Conclusions can be summarized as follows. (1) Neuroendocrine-immune network (NIN)-osteoclast regulatory pathway OPG-RANKL-RANK is one of the mechanisms of “kidney governing bones.” Although kidney-reinforcing therapy is regarded as one of the holistic regulatory mechanisms of the body, characteristic holistic regulation in TCM can be reflected through nonselective regulation of the NIN during kidney reinforcement therapy, which can be used to treat osteoporosis through microadjustments in the microenvironment of the bone marrow. (2) Marrow exhaustion in TCM, which is the state wherein lipocytes in the bone marrow increase whereas other cells decrease, serves as the pathogenesis of osteoporosis brought about by failure of the “kidney governing bones.” (3) The kidney in TCM can be regarded as a complex system comprising multiple functional units in the body, including the unit “governing bones.” Kidney deficiency refers to a deficiency in only one or more units of the kidney system and not the whole system itself, which explains the kidney-reinforcing effect of many herbs; some herbs can treat osteoporosis, but some cannot. Although both classified as kidney-reinforcing agents, the former can resolve failure of the “kidney governing bones” unit while the latter regulates the failure of other units in the kidney system. Despite the current understanding on “kidney governing bones” theory, the mechanism of “kidney governing bones” remains complicated and unresolved. Thus, further studies in this area are warranted.
关键词: kidney governing bones kidney deficiency marrow osteoporosis neuroendocrine-immune network osteoclast regulatory pathway
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《医学前沿(英文)》 2015年 第9卷 第3期 页码 288-303 doi: 10.1007/s11684-015-0412-0
Toll-like receptors (TLRs), which are found in innate immune cells, are essential mediators of rapid inflammatory responses and appropriate T-cell activation in response to infection and tissue damage. Accumulating evidence suggests that TLR signaling is involved in normal hematopoiesis and specific hematologic pathologies. Particular TLRs and their downstream signaling mediators are expressed not only in terminally differentiated innate immune cells but also in early hematopoietic progenitors. Sterile activation of TLR signaling is required to generate early embryonic hematopoietic progenitor cells. In adult animals, TLR signaling directly or indirectly promotes differentiation of myeloid cells at the expense of that of lymphoid cells and the self-renewal of hematopoietic stem cells during infection and tissue damage. Activating mutations of the MyD88 gene, which codes for a key adaptor involved in TLR signaling, are commonly detected in B-cell lymphomas and other B-cell hematopathologies. Dysregulated TLR signaling contributes to the pathogenesis of many hematopoietic disorders, including bone marrow failure, myelodysplastic syndrome, and acute myeloid leukemia. Complete elucidation of the molecular mechanisms by which TLR signaling mediates the regulation of both normal and pathogenic hematopoiesis will prove valuable to the development of targeted therapies and strategies for improved treatment of hematopoietic disorders.
关键词: TLR MyD88 hematopoiesis bone marrow failure leukemia myelodysplastic syndrome
Hemophagocytic lymphohistiocytosis: critical reappraisal of a potentially under-recognized condition
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《医学前沿(英文)》 2013年 第7卷 第4期 页码 492-498 doi: 10.1007/s11684-013-0292-0
Hemophagocytic lymphohistiocytosis (HLH) is an uncommon, potentially life threatening, hyper inflammatory syndrome of diverse etiologies. Cardinal signs include prolonged fever, organomegaly, and persistent unexplained cytopenias. In spite of the well known diagnostic criteria put forth by HLH society, this continues to pose great diagnostic challenge in both pediatric and adult intensive care settings. We describe 4 adult (2 males, 2 females, aged 19, 29, 40, and 17 years) and 3 pediatric (2 males, 1female, aged 1 month, 6 months, and 12 years) patients with secondary HLH who satisfied the HLH-2004 diagnostic criteria. Definite evidence of hemophagocytosis was noted in 4 patients on initial bone marrow examination. The underlying etiologies were as follows: Rickettsia tsutsugamushi (case 1), autoimmune disorder (case 2), systemic onset juvenile idiopathic arthritis (sJIA) (case 3), unknown bite (possibly a venomous snake) (case 4), Plasmodium vivax (case 5), Cytomegalo virus (case 6), and Mycobacterium tuberculosis (case 7). In one patient, hemophagocytosis was presumed to have been exacerbated by administration of granulocyte monocyte colony stimulating factor (GM-CSF) for severe neutropenia. Two patients died with disseminated intravascular coagulation (DIC) and multi organ failure within few days of HLH diagnosis. Immunosuppressive therapy was started in 3 patients, and etoposide was started in one patient only. Due to lack of specificity of diagnostic criteria, diagnosing and differentiating HLH from its closest mimickers like sepsis/septic shock may be quite challenging in critically ill patients. Therefore, increasing awareness among physicians is essential for early diagnosis and effective therapy to reduce the mortality.
关键词: hemophagocytic lymphohistiocytosis diagnosis therapy GM-CSF bone marrow
Lili Zhou, Ping Li, Shiguang Ye, Xiaochen Tang, Junbang Wang, Jie Liu, Aibin Liang
《医学前沿(英文)》 2020年 第14卷 第6期 页码 786-791 doi: 10.1007/s11684-020-0751-3
关键词: anti-CD19 chimeric antigen receptor T cell soft tissue bone marrow relapsed or refractory non-Hodgkin lymphoma
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《医学前沿(英文)》 2015年 第9卷 第4期 页码 468-477 doi: 10.1007/s11684-015-0419-6
Mature T-cell lymphoid malignancies comprise a group of heterogeneous diseases that vary in clinicopathological features, biological behavior, treatment response, and prognosis. Bone marrow (BM) infiltration is more commonly present in mature T-cell lymphoid malignancies compared with their B-cell counterparts and hence important for differential diagnosis. In this study, clinical characteristics and prognostic factors were analyzed in 225 patients with mature T-cell lymphoid malignancies treated in a single institution. These included 29 cases of T-cell lymphoproliferative disorders (T-LPD, all with BM infiltration) and 196 cases of T-/natural-killer-cell lymphoma (T/NKCL, 56 with BM infiltration and 140 without BM infiltration). The estimated 5-year overall survival (OS) rates of T-LPD and T/NKCL were 96.6% and 37.3%, respectively. T-LPD patients were less likely to exhibit poor performance status, advanced disease stage, presence of B symptoms, or abnormal level of serum β-2 microglobulin. With similar pathological characteristics, T/NKCL patients with BM infiltration showed significantly lower response rates and shorter OS than those without BM infiltration (P = 0.0264 and P<0.0001, respectively). Multivariate analysis indicated that poor performance status, advanced disease stage, elevated serum lactate dehydrogenase level, and BM involvement were independent unfavorable prognostic factors. The Glasgow Prognostic Score may be more efficient than the International Prognostic Index in predicting disease outcome in T/NKCL. In conclusion, clinical characteristics may be useful in more effectively stratifying patients with mature T-cell lymphoid malignancies.
关键词: mature T-cell lymphoid malignancies clonal T-cell population bone marrow infiltration prognostic factors
标题 作者 时间 类型 操作
Autologous bone marrow stem cell transplantation for the treatment of ulcerative colitis complicated
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期刊论文
Outcomes of haploidentical bone marrow transplantation in patients with severe aplastic anemia-II that
期刊论文
Pluripotent stem cells exhibiting similar characteristics can be isolated from human fetal bone marrow
FANG Baijun, SONG Yongping, LIN Quande, ZHAO Chunhua, SHI Mingxia
期刊论文
intensity and loading time of direct current electric field on the directional migration of rat bone marrow
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期刊论文
Toll-like receptor signaling in hematopoietic homeostasis and the pathogenesis of hematologic diseases
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期刊论文
Hemophagocytic lymphohistiocytosis: critical reappraisal of a potentially under-recognized condition
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期刊论文
Different sites of extranodal involvement may affect the survival of patients with relapsed or refractory non-Hodgkin lymphoma after chimeric antigen receptor T cell therapy
Lili Zhou, Ping Li, Shiguang Ye, Xiaochen Tang, Junbang Wang, Jie Liu, Aibin Liang
期刊论文