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《医学前沿(英文)》 2014年 第8卷 第1期 页码 106-112 doi: 10.1007/s11684-014-0307-5
Cervical carcinoma is associated with high propensity for local invasion and lymph node metastasis. However, the molecular alterations that drive progression and metastasis of cervical cancer remain unclear. Cellular senescence has been proposed as the mechanism that protects an organism against cancer progression and metastasis. In addition, Twist, a basic helix-loop-helix transcription factor, has been suggested as an oncogene because it is overexpressed in many types of human cancer. This gene also exhibits a positive function in regulating invasion and metastasis. In this study, Twist was strongly and positively expressed in normal tissue, squamous cell carcinoma (SCC) IA--IIA, and SCC IIB--IIIB (4.3%, 44%, and 88.9%, respectively). The strong positive expressions of the senescence marker CBX3 were 39.1%, 32%, and 15.6%, respectively. The strong positive expressions of Twist in the SCC groups with or without lymph node metastasis were 80.8% and 50%. For CBX3, such expressions were 7.7% and 29.5%, respectively. Results also showed that the expression of Twist was inversely correlated with that of CBX3. Moreover, the knockdown of Twist with target siRNA in SiHa triggered the induction of the chromatin marker of the cellular senescence CBX3 and senescence-associated β-galactosidase activity. Our results suggested that the expression of Twist increased during the progression and metastasis of cervical cancer. Furthermore, Twist-induced senescence bypass is important in this process.
关键词: cervical cancer senescence Twist CBX3 lymph node metastasis
《医学前沿(英文)》 2022年 第16卷 第6期 页码 883-895 doi: 10.1007/s11684-022-0919-0
关键词: osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B
Osteopontin is a promoter for hepatocellular carcinoma metastasis: a summary of 10 years of studies
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《医学前沿(英文)》 2014年 第8卷 第1期 页码 24-32 doi: 10.1007/s11684-014-0312-8
In this review, we summarize the novel findings from our series of studies on the leading metastasis-related gene, osteopontin (OPN). In our previous gene expression profiling study, OPN was identified as one of the leading genes associated with the metastasis of hepatocellular carcinoma (HCC). We focused on OPN to evaluate its prognostic values and important roles in HCC metastasis. A retrospective study of large cohorts of HCC patients demonstrated that plasma OPN level was one of the leading independent prognostic factors for HCC patients, even in the early stage of HCC, and could serve as a surrogate serologic biomarker for monitoring the treatment response and tumor recurrence after HCC resection. Using both in vitro and in vivoinvestigations, we found that OPN has an important role in metastasis and tumor growth of HCC and is an attractive potential therapeutic target for combating HCC metastasis. We also found that OPN+ HCC cells have much more amplifications at chromosomal regions, and promoter polymorphisms are important in the regulation of OPN expression and tumor growth and lung metastasis of HCC.
关键词: osteopontin (OPN) hepatocellular carcinoma metastasis prognosis therapeutic target biomarker genetic polymorphism
《医学前沿(英文)》 2022年 第16卷 第4期 页码 551-573 doi: 10.1007/s11684-022-0928-z
关键词: HCC bone osteotropism clinical basic researches advances
Liu LIU MD, PhD, Yaogui NING MM, Chen CHEN MD, Daowen WANG MD, PhD,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 443-446 doi: 10.1007/s11684-009-0079-5
Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
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《医学前沿(英文)》 2017年 第11卷 第2期 页码 214-222 doi: 10.1007/s11684-017-0518-7
MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial?mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer.
Epithelial-to-mesenchymal transition in cancer: complexity and opportunities
Yun Zhang, Robert A. Weinberg
《医学前沿(英文)》 2018年 第12卷 第4期 页码 361-373 doi: 10.1007/s11684-018-0656-6
The cell-biological program termed the epithelial-to-mesenchymal transition (EMT) plays an important role in both development and cancer progression. Depending on the contextual signals and intracellular gene circuits of a particular cell, this program can drive fully epithelial cells to enter into a series of phenotypic states arrayed along the epithelial-mesenchymal phenotypic axis. These cell states display distinctive cellular characteristics, including stemness, invasiveness, drug-resistance and the ability to form metastases at distant organs, and thereby contribute to cancer metastasis and relapse. Currently we still lack a coherent overview of the molecular and biochemical mechanisms inducing cells to enter various states along the epithelial-mesenchymal phenotypic spectrum. An improved understanding of the dynamic and plastic nature of the EMT program has the potential to yield novel therapies targeting this cellular program that may aid in the management of high-grade malignancies.
关键词: epithelial-to-mesenchymal transition cancer metastasis cancer stem cell
Data mining of microarray for differentially expressed genes in liver metastasis from gastric cancer
Ling XU MM, Feng WANG MM, Xuan-Fu XU MD, Wen-Hui MO BM, Rong WAN MD, Chuan-Yong GUO MD, Xing-Peng WANG MD,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 247-253 doi: 10.1007/s11684-010-0027-4
关键词: gastric carcinoma metastasis signal transduction gene chips
Tumor growth and metastasis can be inhibited by maintaining genomic stability in cancer cells
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《医学前沿(英文)》 2015年 第9卷 第1期 页码 57-62 doi: 10.1007/s11684-015-0389-8
The existence of cancer stem cells, stem-like cancer cells (SLCCs), or tumor-initiating cells is considered as the cause of tumor formation and recurrence, indicating the importance of studying novel therapy that targets SLCCs. The origin of SLCCs is controversial because of two competing hypotheses: SLCCs are either transformed from tissue adult stem cells or dedifferentiated from transformed progenitor cells. Our previous research demonstrates that SLCCs are inducible by increasing genomic instability in cancer cells. In this study, to block the emergence of SLCCs, aminoethyl isothiourea (AET), a compound that clears free radicals and is used to protect patients from radioactive exposure, was used as an agent that maintains genomic stability in combination with mitomycin C (MMC), a commonly used chemotherapeutic drug that damages DNA. Using a rabbit tumor model with VX2 hepatic carcinoma, we found that MMC alone increased lung metastases and disadvantaged survival outcome, but the combination of MMC and AET reversed this effect and even prolonged overall survival. Moreover, in a VX2 xenograft model by immunocompromised mice, MMC alone enriched tumor-initiating cells, but the administration of MMC in combination with AET eliminated tumor cells effectively. Furthermore, MMC alone enhanced genomic instability, but MMC combined with AET attenuated the extent of genomic instability in primary VX2 tumor tissue. Taken together, our data suggest that the genomic protector AET can inhibit the induction of SLCCs, and this combination treatment by AET and cytotoxic agents should be considered as a promising strategy for future clinical evaluation.
关键词: rabbit VX2 liver tumor mitomycin C AET stem-like cancer cells genomic instability
The predictive value of chromosome 8p deletion for metastasis of hepatocellular carcinoma: a summary
QIN Lunxiu, TANG Zhaoyou, GUAN Xinyuan, YE Qinghai, JIA Huliang, REN Ning
《医学前沿(英文)》 2008年 第2卷 第3期 页码 211-215 doi: 10.1007/s11684-008-0041-y
关键词: sensitive genome-wide prediction genome-wide microsatellite frequency Hepatocellular carcinoma
Application progress of lymphography in oncology
Yang YU, Jibin LIU, Lixue YIN
《医学前沿(英文)》 2009年 第3卷 第1期 页码 13-19 doi: 10.1007/s11684-009-0016-7
关键词: lymphography lymphatic metastasis sentinel lymph node biopsy
The impact of hypoxia in hepatocellular carcinoma metastasis
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《医学前沿(英文)》 2014年 第8卷 第1期 页码 33-41 doi: 10.1007/s11684-013-0301-3
Hypoxia is a common phenomenon in hepatocellular carcinoma (HCC). Hypoxia stabilizes transcription factor, hypoxia-inducible factor (HIF), to activate gene transcription. Expression of HIF is closely associated with metastasis and poor prognosis in HCC. HIF mediates expression of genes that are involved in every step of HCC metastasis including epithelial-mesenchymal transition, invasion of the extracellular matrix, intravasation, extravasation, and secondar y growth of the metastases. Because HIF is the central regulator of HCC metastasis, HIF inhibitors are attractive tools when used alone or as combined treatment to curb HCC metastasis. This review will summarize the current findings on the impact of hypoxia/HIF in HCC, with a particular focus on cancer metastasis.
关键词: hypoxia hepatocellular carcinoma (HCC) metastasis hypoxia-inducible factor (HIF)
Hilar cholangiocarcinoma: Pathology and tumor biology
Dong KUANG, Guo-Ping WANG,
《医学前沿(英文)》 2010年 第4卷 第4期 页码 371-377 doi: 10.1007/s11684-010-0130-6
关键词: hilar cholangiocarcinoma morphology primary sclerosing cholangitis metastasis growth
Lihui WANG, Lianhong LI, Shen LV, Shujun FAN, Li ZHAN, Bo WANG, Zhong ZHANG
《医学前沿(英文)》 2009年 第3卷 第2期 页码 164-170 doi: 10.1007/s11684-009-0038-1
关键词: epithelial-mesenchymal transition vascular endothelial cell growth factor matrix metalloproteinase-9 cyclooxygenase-2 higher microvascular density breast cancer
Bioinformatic exploration of MTA1-regulated gene networks in colon cancer
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《医学前沿(英文)》 2016年 第10卷 第2期 页码 178-182 doi: 10.1007/s11684-016-0442-2
Metastasis-associated gene 1 (MTA1) controls a series of biological processes in tumor progression. Tumor progression is a complex process regulated by a gene network. The global cancer gene regulatory network must be analyzed to determine the position of MTA1 in the molecular network and its cooperative genes by further exploring the biological functions of this gene. We used TCGA data sets and GeneCards database to screen MTA1-related genes. GO and KEGG pathway analyses were conducted with DAVID and gene network analysis via STRING and Cytoscape. Results showed that in the development of colon cancer, MTA1 is linked to certain signal pathways, such as Wnt/Notch/nucleotide excision repair pathways. The findings also suggested that MTA1 demonstrates the closest relationship in a coregulation process with the key molecules AKT1, EP300, CREBBP, SMARCA4, RHOA, and CAD. These results lead MTA1 exploration to an in-depth investigation in different directions, such as Wnt, Notch, and DNA repair.
关键词: metastasis-associated gene 1 colon cancer bioinformatics
标题 作者 时间 类型 操作
Correlation of Twist upregulation and senescence bypass during the progression and metastasis of cervical
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期刊论文
Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance
期刊论文
Osteopontin is a promoter for hepatocellular carcinoma metastasis: a summary of 10 years of studies
null
期刊论文
Bone metastasis of hepatocellular carcinoma: facts and hopes from clinical and translational perspectives
期刊论文
Effect of atorvastatin on tumor growth and metastasis in a breast cancer cell xenograft model and its
Liu LIU MD, PhD, Yaogui NING MM, Chen CHEN MD, Daowen WANG MD, PhD,
期刊论文
Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
null
期刊论文
Epithelial-to-mesenchymal transition in cancer: complexity and opportunities
Yun Zhang, Robert A. Weinberg
期刊论文
Data mining of microarray for differentially expressed genes in liver metastasis from gastric cancer
Ling XU MM, Feng WANG MM, Xuan-Fu XU MD, Wen-Hui MO BM, Rong WAN MD, Chuan-Yong GUO MD, Xing-Peng WANG MD,
期刊论文
Tumor growth and metastasis can be inhibited by maintaining genomic stability in cancer cells
null
期刊论文
The predictive value of chromosome 8p deletion for metastasis of hepatocellular carcinoma: a summary
QIN Lunxiu, TANG Zhaoyou, GUAN Xinyuan, YE Qinghai, JIA Huliang, REN Ning
期刊论文
Lymphatic metastasis is related to the epithelial-mesenchymal transition and expressions of VEGF, MMP
Lihui WANG, Lianhong LI, Shen LV, Shujun FAN, Li ZHAN, Bo WANG, Zhong ZHANG
期刊论文