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The microRNA, miR-29c, participates in muscle development through targeting the

Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI

《农业科学与工程前沿(英文)》 2015年 第2卷 第4期   页码 311-317 doi: 10.15302/J-FASE-2015075

摘要: Previous studies indicated that miR-29c is important for muscle development in mice and human, but its role in pigs is unknown. In this study, we detected the expression of miR-29c in Meishan longissimus lumborum (LL) muscle. The results showed that miR-29c was gradually upregulated during development of skeletal muscle in pig. Moreover, the expression of and genes, which were confirmed to be targeted by miR-29c in mice, was decreased along with muscle development. Furthermore, the expression level of miR-29c was significantly higher in adult Meishan pigs than Large White pigs, while the expression of and genes was significantly lower in Meishan pigs. These results indicated that the expression pattern of miR-29c was opposite to that of and genes in pigs. Also, the luciferase assay indicated that miR-29s can target the gene in pigs. In addition, we identified a T to C mutation in the primary transcript of miR-29c, which was associated with the postmortem muscle pH in pigs. Based on these results, we concluded that miR-29c is also important in skeletal muscle development of pigs.

关键词: pig     miR-29c     skeletal muscle     expression     SNP    

Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and

null

《医学前沿(英文)》 2014年 第8卷 第4期   页码 456-463 doi: 10.1007/s11684-014-0353-z

摘要:

microRNAs (miRNAs) have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c (miR-200c) on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200c compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR-200c could inhibit migration and invasion by epithelial-mesenchymal transition.

关键词: miR-200c     proliferation     migration     invasion     prostate cancer     Du145 cell     ZEB1    

hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析

徐青,付子毅,吴小莉,皇甫玉爽,凌静

《中国工程科学》 2014年 第16卷 第5期   页码 99-104

摘要:

应用实时定量聚合酶链式反应(PCR)技术检测子宫肌瘤组织中的hsa-miR-197 表达水平,并与配对正常子宫肌细胞对比。通过miRbase、UCSC、NCBI等在线数据库获取并分析hsa-miR-197 序列保守性及其基因组特征。选择miRanda、MirTarget2 及TargetScan 三个在线数据库预测hsa-miR-197 的靶基因并取交集以便后续分析。hsa-miR-197 的功能较广泛,参与肿瘤发生的多种生物学过程,提示hsa-miR-197 可能与子宫肌瘤的发病机制密切相关。

关键词: 子宫肌瘤     hsa-miR-197     生物信息学     靶基因    

Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer

null

《医学前沿(英文)》 2017年 第11卷 第2期   页码 214-222 doi: 10.1007/s11684-017-0518-7

摘要:

MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial?mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer.

关键词: ovarian cancer     metastasis     miR-9     E-cadherin    

-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation

Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,

《工程(英文)》 doi: 10.1016/j.eng.2023.09.007

摘要: Traditional Chinese medicine (TCM) can help prevent or treat diseases; however, there are few studies on the active substances of TCM. For example, Lycium barbarum L. has been proven to be effective in treating osteoporosis for thousands of years, but its active substance remains to be unknown. Prompted by the efforts to modernize TCM, the present study focused on the novel active substance of Lycium barbarum L. to reinforce kidney essence to produce bone marrow. Illumina deep sequencing analysis and stem-loop polymerase chain reaction (PCR) assay revealed that miR162a, a Lycium barbarum L.-derived microRNA, can pass through the gastrointestinal tract to target the bone marrow in mice. Immunofluorescence staining showed that miR162a was absorbed through systemic RNA interference defective transmembrane family member 1 (SIDT1) in the stomach. Bioinformatics prediction and luciferase reporter assay identified that miR162a targeted nuclear receptor corepressor (NcoR). Alizarin red staining and micro-computed tomography (microCT) confirmed that miR162a promoted osteogenic differentiation in bone marrow mesenchymal stem cells, zebrafish, and a mouse model of osteoporosis. In addition, transgenic Nicotiana benthamiana (N. benthamiana) leaves overexpressing miR162a were developed by agrobacterium infiltration method. microCT and tartrate-resistant acid phosphatase staining confirmed that transgenic N. benthamiana leaves effectively protected against osteoporosis in mice. Our study mechanistically explains how Lycium barbarum L. improves osteoporosis and supports that Lycium barbarum L. reinforces kidney essence, thereby strengthening the bone. miR162a expressed by transgenic plants may represent a novel and safe treatment for human osteoporosis.

关键词: Traditional Chinese medicine     Lycium barbarum     L     miR162a     Osteoporosis     Nuclear receptor corepressor     Transgenic plants    

Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes

Huiwen Ren, Can Wu, Ying Shao, Shuang Liu, Yang Zhou, Qiuyue Wang

《医学前沿(英文)》 2020年 第14卷 第5期   页码 642-650 doi: 10.1007/s11684-019-0719-3

摘要: This study aimed to investigate the correlation between serum miR-154-5p and urinary albumin to creatinine ratio (UACR) in patients with type 2 diabetes mellitus (T2DM) and the association with biomarkers of inflammation and fibrosis in diabetic kidney disease (DKD). A total of 390 patients with T2DM were divided into three groups: normal albuminuria (UACR<30 mg/g, =136, NA), microalbuminuria (UACR at 30–300 mg/g, =132, MA), and clinical albuminuria (UACR>300 mg/g, =122, CA). Circulating miR-154-5p, inflammatory (C-reactive protein (CRP); erythrocyte sedimentation rate (ESR); and tumor necrosis factor-α (TNF-α) and fibrotic markers (vascular endothelial growth factor (VEGF); transforming growth factor-β1 (TGF-β1); and fibronectin (FN)), and other biochemical indicators were assessed via real-time PCR, enzyme-linked immunosorbent assay, and chemiluminescence assay in patients with T2DM and 138 control subjects (NC). UACR, miR-154-5p, glycated hemoglobin (HbA1c), serum creatinine (sCr), blood urea nitrogen (BUN), ESR, CRP, VEGF, TNF-α, TGF-β1, and FN were significantly higher and the estimated glomerular filtration rate (eGFR) was significantly lower in NA, MA, and CA groups than in NC subjects ( <0.05). Elevated levels of UACR and miR-154-5p were directly correlated with HbA1c, sCr, BUN, ESR, CRP, VEGF, TNF-α, TGF-β1, and FN and negatively correlated with eGFR ( <0.05). miR-154-5p, HbA1c, sCr, BUN, eGFR, ESR, CRP, VEGF, TNF-α, TGF-β1, and FN were important factors affecting UACR. These findings indicated that elevated serum miR-154-5p is significantly correlated with high UACR in patients with T2DM and may offer a novel reference for the early diagnosis of DKD.

关键词: type 2 diabetes mellitus     diabetic kidney disease     miR-154-5p     urinary albumin to creatinine ratio    

Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR

《医学前沿(英文)》   页码 924-938 doi: 10.1007/s11684-023-1004-z

摘要: Long noncoding RNAs (lncRNAs) play a crucial regulatory role in the development and progression of multiple cancers. However, the potential mechanism by which lncRNAs affect the recurrence and metastasis of ovarian cancer remains unclear. In the current study, the lncRNA LOC646029 was markedly downregulated in metastatic ovarian tumors compared with primary tumors. Gain- and loss-of-function assays demonstrated that LOC646029 inhibits the proliferation, invasiveness, and metastasis of ovarian cancer cells in vivo and in vitro. Moreover, the downregulation of LOC646029 in metastatic ovarian tumors was strongly correlated with poor prognosis. Mechanistically, LOC646029 served as a miR-627-3p sponge to promote the expression of Sprouty-related EVH1 domain-containing protein 1, which is necessary for suppressing tumor metastasis and inhibiting KRAS signaling. Collectively, our results demonstrated that LOC646029 is involved in the progression and metastasis of ovarian cancer, which may be a potential prognostic biomarker.

关键词: ovarian cancer     lncRNA LOC646029     metastasis     microRNA 627-3p     SPRED1    

Influence of mass air flow ratio on gas-particle flow characteristics of a swirl burner in a 29 MW pulverized

Rong YAN, Zhichao CHEN, Shuo GUAN, Zhengqi LI

《能源前沿(英文)》 2021年 第15卷 第1期   页码 68-77 doi: 10.1007/s11708-020-0697-9

摘要: In a gas/particle two-phase test facility, a three-component particle-dynamics anemometer was used to measure the characteristics of gas/particle two-phase flows in a 29 megawatt (MW) pulverized coal industrial boiler equipped with a new type of swirling pulverized coal burner. The distributions of three-dimensional gas/particle velocity, particle volume flux, and particle size distribution were measured under different working conditions. The mean axial velocity and the particle volume flux in the central region of the burner outlet were found to be negative. This indicated that a central recirculation zone was formed in the center of the burner. In the central recirculation zone, the absolute value of the mean axial velocity and the particle volume flux increased when the external secondary air volume increased. The size of the central reflux zone remained stable when the air volume ratio changed. Along the direction of the jet, the peak value formed by the tertiary air gradually moved toward the center of the burner. This tertiary air was mixed with the peak value formed by the air in the adiabatic combustion chamber after the cross-section of / = 0.7. Large particles were concentrated near the wall area, and the particle size in the recirculation zone was small.

关键词: industrial pulverized coal boiler     swirl burner     air/particle flow     particle dynamic analyzer (PDA)    

lncR-GAS5 upregulates the splicing factor to impair endothelial autophagy, leading to atherogenesis

《医学前沿(英文)》 2023年 第17卷 第2期   页码 317-329 doi: 10.1007/s11684-022-0931-4

摘要: Long noncoding RNAs (lncRNAs) play a critical role in the regulation of atherosclerosis. Here, we investigated the role of the lncRNA growth arrest-specific 5 (lncR-GAS5) in atherogenesis. We found that the enforced expression of lncR-GAS5 contributed to the development of atherosclerosis, which presented as increased plaque size and reduced collagen content. Moreover, impaired autophagy was observed, as shown by a decreased LC3II/LC3I protein ratio and an elevated P62 level in lncR-GAS5-overexpressing human aortic endothelial cells. By contrast, lncR-GAS5 knockdown promoted autophagy. Moreover, serine/arginine-rich splicing factor 10 (SRSF10) knockdown increased the LC3II/LC3I ratio and decreased the P62 level, thus enhancing the formation of autophagic vacuoles, autolysosomes, and autophagosomes. Mechanistically, lncR-GAS5 regulated the downstream splicing factor SRSF10 to impair autophagy in the endothelium, which was reversed by the knockdown of SRSF10. Further results revealed that overexpression of the lncR-GAS5-targeted gene miR-193-5p promoted autophagy and autophagic vacuole accumulation by repressing its direct target gene, SRSF10. Notably, miR-193-5p overexpression decreased plaque size and increased collagen content. Altogether, these findings demonstrate that lncR-GAS5 partially contributes to atherogenesis and plaque instability by impairing endothelial autophagy. In conclusion, lncR-GAS5 overexpression arrested endothelial autophagy through the miR-193-5p/SRSF10 signaling pathway. Thus, miR-193-5p/SRSF10 may serve as a novel treatment target for atherosclerosis.

关键词: lncR-GAS5     miR-193-5p     splicing factor SRSF10     autophagy     atherogenesis    

Valveless pumping using a two-stage impedance pump

C.-C. LEE, Y. A. ABAKR, K.-C. WOO

《机械工程前沿(英文)》 2013年 第8卷 第3期   页码 311-318 doi: 10.1007/s11465-013-0270-x

摘要:

Impedance pump is defined as a type of valveless pumping mechanism, where an elastic tube is joined with a tube of different impedance, a periodic asymmetrical compression on part of the elastic tube will produce a unidirectional flow. Numerical and experimental studies had been conducted in the past, but little experimental work was found to exploit the principle of multi-stage impedance pumping for fluid transportation. This paper concentrates on the fluid behavior for an open-loop two-stage system experimentally. The aim of this work is to have an insight on the deliverable head and pumping rate of an open-loop two-stage impedance pumping system. This paper generally investigates the effect of different parameters towards the generation of unidirectional flows and composite flow of two opposite directions. Results obtained are of significant values.

关键词: valveless pumping     open-loop     two-stage     unidirectional     composite    

基于S3C2410的GPRS数据终端的设计

孙学用

《中国工程科学》 2007年 第9卷 第12期   页码 94-96

摘要: 提出了以S3C2410处理器为核心,以SIM100-E为GPRS通信模块,构建了一套嵌入式Internet数据终端的方案,对其硬件构成及工作流程进行了讨论,描述了其软件层次结构,并详细讲解了基于该平台的

关键词: GPRS;嵌入式Internet;S3C2410;SIM100-E    

Bioinspired C/TiO

Jian LI, Likun GAO, Wentao GAN

《农业科学与工程前沿(英文)》 2017年 第4卷 第4期   页码 459-464 doi: 10.15302/J-FASE-2017178

摘要: butterfly wings were replicated by a sol-gel method and a calcination process, which could take advantage of the spatial features of the wing to enhance their photocatalytic properties. Hierarchical structures of -carbon-TiO (PP-C-TiO ) were confirmed by SEM observations. By applying the Brunauer-Emmett-Teller method, it was concluded that in the presence of wings the product shows higher surface area with respect to the pure TiO made in the absence of the wings. The higher specific surface area is also beneficial for the improvement of photocatalytic property. Furthermore, the conduction and valence bands of the PP-C-TiO are more negative than the corresponding bands of pure TiO , allowing the electrons to migrate from the valence band to the conduction band upon absorbing visible light. That is, the presence of C originating from wings in the PP-C-TiO could extend the photoresponsiveness to visible light. This strategy provides a simple method to fabricate a high-performance photocatalyst, which enables the simultaneous control of the morphology and carbon element doping.

关键词: bioinspired     butterfly wings     C/TiO2     photocatalyst     visible light    

MicroRNA-148b promotes proliferation of hair follicle cells by targeting

Wanbao YANG,Qinqun LI,Bo SU,Mei YU

《农业科学与工程前沿(英文)》 2016年 第3卷 第1期   页码 72-80 doi: 10.15302/J-FASE-2016089

摘要: MicroRNAs (miRNAs), small non-coding RNAs, are involved in many aspects of biological processes. Previous studies have indicated that miRNAs are important for hair follicle development and growth. In our study, we found by qRT-PCR that miR-148b was significantly upregulated in sheep wool follicle bulbs in anagen phase compared with the telogen phase of the hair follicle cycle. Overexpression of miR-148b promoted proliferation of both HHDPC and HHGMC. By using the TOPFlash system we demonstrated that miR-148b could activate Wnt/β-catenin pathway and , , and were consistently upregulated accordingly. Furthermore, transcript factor nuclear factor of activated T cells type 5 ( ) and were predicted to be the target of miR-148b and this was substantiated using a Dual-Luciferase reporter system. Subsequently was further identified as the target of miR-148b using western blotting. These results were considered to indicate that miR-148b could activate the Wnt/β-catenin signal pathway by targeting to promote the proliferation of human hair follicle cells.

关键词: miR-148b     hair follicle     proliferation     NFAT5     Wnt10b    

Antithrombin deficiency and decreased protein C activity in a young man with venous thromboembolism:

null

《医学前沿(英文)》 2018年 第12卷 第3期   页码 319-323 doi: 10.1007/s11684-017-0553-4

摘要:

Antithrombin and protein C are two crucial members in the anticoagulant system and play important roles in hemostasis. Mutations in and lead to deficiency or dysfunction of the two proteins, which could result in venous thromboembolism (VTE). Here, we report a Chinese 22-year-old young man who developed recurrent and serious VTE in cerebral veins, visceral veins, and deep veins of the lower extremity. Laboratory tests and direct sequencing of and were conducted for the patient and his family members. Coagulation tests revealed that the patient presented type I antithrombin deficiency combined with decreased protein C activity resulting from a small insertion mutation c.848_849insGATGT in and a short deletion variant c.572_574delAGA in . This combination of the two mutations was absent in 400 healthy subjects each from southern and northern China. Then, we summarized all the mutations of the and gene reported in the Chinese Han population. This study demonstrates that the combination of antithrombin deficiency and decreased protein C activity can result in severe VTE and that the coexistence of different genetic factors may increase the risk of VTE.

关键词: antithrombin deficiency     protein C activity     mutation     variant     venous thromboembolism     anticoagulants    

Atomistic simulations of plasma catalytic processes

Erik C. Neyts

《化学科学与工程前沿(英文)》 2018年 第12卷 第1期   页码 145-154 doi: 10.1007/s11705-017-1674-7

摘要: There is currently a growing interest in the realisation and optimization of hybrid plasma/catalyst systems for a multitude of applications, ranging from nanotechnology to environmental chemistry. In spite of this interest, there is, however, a lack in fundamental understanding of the underlying processes in such systems. While a lot of experimental research is already being carried out to gain this understanding, only recently the first simulations have appeared in the literature. In this contribution, an overview is presented on atomic scale simulations of plasma catalytic processes as carried out in our group. In particular, this contribution focusses on plasma-assisted catalyzed carbon nanostructure growth, and plasma catalysis for greenhouse gas conversion. Attention is paid to what can routinely be done, and where challenges persist.

关键词: atomic scale simulation     plasma-catalyst    

标题 作者 时间 类型 操作

The microRNA, miR-29c, participates in muscle development through targeting the

Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI

期刊论文

Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and

null

期刊论文

hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析

徐青,付子毅,吴小莉,皇甫玉爽,凌静

期刊论文

Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer

null

期刊论文

-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation

Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,

期刊论文

Correlation between serum miR-154-5p and urinary albumin excretion rates in patients with type 2 diabetes

Huiwen Ren, Can Wu, Ying Shao, Shuang Liu, Yang Zhou, Qiuyue Wang

期刊论文

Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR

期刊论文

Influence of mass air flow ratio on gas-particle flow characteristics of a swirl burner in a 29 MW pulverized

Rong YAN, Zhichao CHEN, Shuo GUAN, Zhengqi LI

期刊论文

lncR-GAS5 upregulates the splicing factor to impair endothelial autophagy, leading to atherogenesis

期刊论文

Valveless pumping using a two-stage impedance pump

C.-C. LEE, Y. A. ABAKR, K.-C. WOO

期刊论文

基于S3C2410的GPRS数据终端的设计

孙学用

期刊论文

Bioinspired C/TiO

Jian LI, Likun GAO, Wentao GAN

期刊论文

MicroRNA-148b promotes proliferation of hair follicle cells by targeting

Wanbao YANG,Qinqun LI,Bo SU,Mei YU

期刊论文

Antithrombin deficiency and decreased protein C activity in a young man with venous thromboembolism:

null

期刊论文

Atomistic simulations of plasma catalytic processes

Erik C. Neyts

期刊论文