Search | Engineering

订阅投稿

首页工程期刊工程焦点工程成就工程前沿关于我们 English

资源类型

期刊论文 39

年份

2023 2

2022 2

2021 6

2020 1

2019 2

2018 2

2017 2

2016 2

2015 4

2014 1

2013 1

2012 2

2009 2

2008 4

2007 6

展开︾

关键词

原子力显微镜 4

纳米加工 2

360°表征 1

SIM 1

三维原子力显微镜 1

三维形貌重建 1

乳液 1

二次谐波产生（SHG）-SIM 1

二氧化硅 1

产品设计 1

关键尺寸 1

分子间和表面间相互作用 1

分散机理 1

单晶铜 1

周期性纳米结构 1

多光子结构光照明超分辨显微成像 1

宫内节育器；盆腔炎性疾病；细菌生物膜；SEM 1

工程过程 1

扩展背向探测；增强探测；非线性荧光显微镜；硅光电倍增管阵列 1

展开︾

检索范围：

排序：展示方式：

Applications of atomic force microscopy in immunology

Jiping Li, Yuying Liu, Yidong Yuan, Bo Huang

《医学前沿（英文）》 2021年第15卷第1期页码 43-52 doi: 10.1007/s11684-020-0769-6

摘要： Cellular mechanics, a major regulating factor of cellular architecture and biological functions, responds to intrinsic stresses and extrinsic forces exerted by other cells and the extracellular matrix in the microenvironment. Cellular mechanics also acts as a fundamental mediator in complicated immune responses, such as cell migration, immune cell activation, and pathogen clearance. The principle of atomic force microscopy (AFM) and its three running modes are introduced for the mechanical characterization of living cells. The peak force tapping mode provides the most delicate and desirable virtues to collect high-resolution images of morphology and force curves. For a concrete description of AFM capabilities, three AFM applications are discussed. These applications include the dynamic progress of a neutrophil-extracellular-trap release by neutrophils, the immunological functions of macrophages, and the membrane pore formation mediated by perforin, streptolysin O, gasdermin D, or membrane attack complex.

关键词： cellular mechanics atomic force microscopy neutrophil extracellular trap macrophage phagocytosis pore formation

HTML PDF 收藏

topology optimization for tailoring the ratio between two flexural eigenfrequencies of atomic force microscopy

Qi XIA,Tao ZHOU,Michael Yu WANG,Tielin SHI

《机械工程前沿（英文）》 2014年第9卷第1期页码 50-57 doi: 10.1007/s11465-014-0286-x

摘要：

In an operation mode of atomic force microscopy that uses a higher eigenmode to determine the physical properties of material surface, the ratio between the eigenfrequency of a higher flexural eigenmode and that of the first flexural eigenmode was identified as an important parameter that affects the sensitivity and accessibility. Structure features such as cut-out are often used to tune the ratio of eigenfrequencies and to enhance the performance. However, there lacks a systematic and automatic method for tailoring the ratio. In order to deal with this issue, a shape and topology optimization problem is formulated, where the ratio between two eigenfrequencies is defined as a constraint and the area of the cantilever is maximized. The optimization problem is solved via the level set based method.

关键词： atomic force microscopy cantilever probe eigenfrequency optimization

HTML PDF 收藏

Three-dimensional reconstruction of light microscopy image sections: present and future

null

《医学前沿（英文）》 2015年第9卷第1期页码 30-45 doi: 10.1007/s11684-014-0337-z

摘要：

Three-dimensional (3D) image reconstruction technologies can reveal previously hidden microstructures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.

关键词： microtomy 3D imaging computer-assisted image processing 3D printing tissue scaffold

HTML PDF 收藏

Review: Tip-based vibrational spectroscopy for nanoscale analysis of emerging energy materials

Amun JARZEMBSKI, Cedric SHASKEY, Keunhan PARK

《能源前沿（英文）》 2018年第12卷第1期页码 43-71 doi: 10.1007/s11708-018-0524-8

摘要： Vibrational spectroscopy is one of the key instrumentations that provide non-invasive investigation of structural and chemical composition for both organic and inorganic materials. However, diffraction of light fundamentally limits the spatial resolution of far-field vibrational spectroscopy to roughly half the wavelength. In this article, we thoroughly review the integration of atomic force microscopy (AFM) with vibrational spectroscopy to enable the nanoscale characterization of emerging energy materials, which has not been possible with far-field optical techniques. The discussed methods utilize the AFM tip as a nanoscopic tool to extract spatially resolved electronic or molecular vibrational resonance spectra of a sample illuminated by a visible or infrared (IR) light source. The absorption of light by electrons or individual functional groups within molecules leads to changes in the sample’s thermal response, optical scattering, and atomic force interactions, all of which can be readily probed by an AFM tip. For example, photothermal induced resonance (PTIR) spectroscopy methods measure a sample’s local thermal expansion or temperature rise. Therefore, they use the AFM tip as a thermal detector to directly relate absorbed IR light to the thermal response of a sample. Optical scattering methods based on scanning near-field optical microscopy (SNOM) correlate the spectrum of scattered near-field light with molecular vibrational modes. More recently, photo-induced force microscopy (PiFM) has been developed to measure the change of the optical force gradient due to the light absorption by molecular vibrational resonances using AFM’s superb sensitivity in detecting tip-sample force interactions. Such recent efforts successfully breech the diffraction limit of light to provide nanoscale spatial resolution of vibrational spectroscopy, which will become a critical technique for characterizing novel energy materials.

关键词： vibrational spectroscopy atomic force microscopy photo-thermal induced resonance scanning near-field optical microscopy tip-enhanced Raman spectroscopy photo-induced force microscopy molecular resonances surface phonon polaritons energy materials

HTML PDF 收藏

Atomic force microscopy study on the microtopography of natural organic matter and newly formed hydrous

GUO Jin, MA Jun, SHI Xuehua

《环境科学与工程前沿（英文）》 2007年第1卷第2期页码 251-256 doi: 10.1007/s11783-007-0043-z

摘要： To understand the water purification mechanism of potassium permanganate as a coagulation-aid during the preoxidation process, the microtopography of its reductive products, the newly formed hydrous manganese dioxide and the aged hydrous manganese dioxide, was investigated. The morphology of natural organic matter (NOM) adsorbed by the newly formed hydrous manganese dioxide was also compared with that of NOM alone. By using the tapping mode atomic force microscopy (AFM), the observation results show that the newly formed hydrous manganese dioxide possess a perforated sheet (with a thickness of 0 1.75 nm) as well as some spherical particle structures compared with the hydrous manganese dioxide with 2 h aging time, which demonstrated that the newly formed hydrous manganese dioxide had a large surface area and adsorption capacity. When 1 mmol/L newly formed hydrous manganese dioxide was added, the microtopography of NOM molecules shifted from a loosely dispersed pancake shape (with adsorption height of 5 8.5 nm) to a densely dispersed and uniform spherical structure. These results provide a valid proof that it is the perfect adsorption capability of the newly formed hydrous manganese dioxide that might result in the coagulation aid effect of potassium permanganate preoxidation.

关键词： spherical microtopography potassium permanganate uniform spherical morphology

HTML PDF 收藏

基于谐波的多光子扫描结构光照明超分辨显微成像

汪磊, 郑晓敏, 周洁, 王美婷, 陈嘉杰, 曾佑君, 许改霞, 王颖, 邱海霞, 邵永红, 屈军乐, 高志, 顾瑛

《工程（英文）》 2022年第16卷第9期页码 65-72 doi: 10.1016/j.eng.2021.12.010

摘要： style="text-align: justify;">本文提出了一种利用多光子非线性效应的多光子结构光照明超分辨显微成像（multiphoton-structured illumination microscopy

关键词：超分辨显微成像结构光照明超分辨显微成像多光子结构光照明超分辨显微成像 SIM 二次谐波产生（SHG）-SIM

HTML PDF 收藏

Surface modification of cellulose nanocrystals

WANG Neng, DING Enyong, CHENG Rongshi

《化学科学与工程前沿（英文）》 2007年第1卷第3期页码 228-232 doi: 10.1007/s11705-007-0041-5

摘要： In order to improve the dispersibility of cellulose nanocrystal (CNC) particles, three different grafted reactions of acetylation, hydroxyethylation and hydroxypropylation were introduced to modify the CNC surface. The main advantages of these methods were the simple and easily controlled reaction conditions, and the dispersibility of the resulting products was distinctly improved. The properties of the modified CNC were characterized by means of Fourier transform infrared spectroscopy (FT-IR), C nuclear magnetic resonance (NMR), transmission electron microscopy (TEM) and thermogravimetric analyses (TGA). The results indicated that after desiccation, the modification products could be dispersed again in the proper solvents by ultrasonic treatments, and the diameter of their particles had no obvious changes. However, their thermal degradation behaviors were quite different. The initial decomposition temperature of the modified products via hydroxyethylation or hydroxypropylation was lower than that of modified products via acetylation.

关键词： acetylation degradation transform microscopy desiccation

HTML PDF 收藏

Analysis of behavior of melted dark green silty soil

TANG Yiqun, ZHOU Nianqing, YANG Ping, SHEN Feng

《结构与土木工程前沿（英文）》 2008年第2卷第3期页码 242-245 doi: 10.1007/s11709-008-0022-x

摘要： The behavior of dark green silty soil of the 6th storey is studied by means of scanning electron microscopy (SEM). The shape, size and contact condition of the basic elements of the soil microstructure, and the shape and the size of the pore are further studied. In addition, a comparative study is made to analyze the experiment results of Cu and dynamic test. The mechanic characteristics of common soil and those of melted soil are presented and their influences on dark silty soil after being frozen are theoretically illustrated from a microscopic perspective.

关键词： electron microscopy mechanic microscopic perspective addition dynamic

HTML PDF 收藏

光学显微镜分辨率提升至亚纳米级

Chris Palmer

《工程（英文）》 2023年第31卷第12期页码 11-14 doi: 10.1016/j.eng.2023.11.002

HTML PDF 收藏

Preparation of solar selective absorbing CuO coating for medium temperature application

HUANG Qunwu, WANG Yiping, LI Jinhua

《化学科学与工程前沿（英文）》 2007年第1卷第3期页码 256-260 doi: 10.1007/s11705-007-0046-0

摘要： A new method of preparing CuO solar selective absorbing coating for medium temperature is presented. After pretreatment, brass was overlaid with CuO by chemical plating. The effects of reactant concentration, reaction temperature and reaction time on the absorptivity of CuO coating were investigated. The optimized condition of preparing CuO coating was obtained. The CuO coating was analyzed with X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). In order to prolong the period of use, the CuO coating was protected by TiO. The experiment shows that the TiO/CuO coating is more heat-resistant, acid-resistant, and wear resistant than CuO coating, without losing absorptivity markedly. The TiO coating can reduce emissivity and protect the CuO coating.

关键词： photoelectron spectroscopy temperature pretreatment absorptivity microscopy

HTML PDF 收藏

Visualization of vascular ultrastructure during osteogenesis by tissue engineering technique

ZHANG Kaigang, ZENG Bingfang, ZHANG Changqing

《医学前沿（英文）》 2007年第1卷第2期页码 181-184 doi: 10.1007/s11684-007-0034-2

摘要： The aim of this paper was to observe and visualize the changes in osteoblasts by electron microscopy during osteogenesis using tissue engineering technique. We also studied the feasibility of improving tissue vascularization of th

关键词： feasibility engineering osteogenesis vascularization microscopy

HTML PDF 收藏

Experimental flexural behavior of SMA-FRP reinforced concrete beam

Adeel ZAFAR, Bassem ANDRAWES

《结构与土木工程前沿（英文）》 2013年第7卷第4期页码 341-355 doi: 10.1007/s11709-013-0221-y

摘要： The most critical drawback in currently used steel reinforcement in reinforced concrete (RC) structures is susceptibility to accumulation of plastic deformation under excessive loads. Many concrete structures due to damaged (yielded) steel reinforcement have undergone costly repairs and replacements. This research presents a new type of shape memory alloy (SMA)-based composite reinforcement with ability to withstand high elongation while exhibiting pseudo-elastic behavior. In this study, small diameter SMA wires are embedded in thermoset resin matrix with or without additional glass fibers to develop composite reinforcement. Manufacturing technique of new proposed composite is validated using microscopy images. The proposed SMA-FRP composite square rebars are first fabricated and then embedded in small scale concrete T-beam. 3-point bending test is conducted on manufactured RC beam using a cyclic displacement controlled regime until failure. It is found that the SMA-FRP composite reinforcement is able to enhance the performance of concrete member by providing re-centering and crack closing capability.

关键词： re-centering shape memory alloys concrete composite fiber reinforced polymer scanning electron microscopy

HTML PDF 收藏

Case report of neurofibromatosis type 1 combined with primary ciliary dyskinesia

《医学前沿（英文）》 2021年第15卷第6期页码 933-937 doi: 10.1007/s11684-021-0860-7

摘要： Neurofibromatosis (NF) is a genetic disease in which the lungs are rarely involved. However, in NF cases with lung involvement, chest computed tomography may show bilateral basal reticulations, apical bullae, and cysts without bronchiectasis. Herein, we report a patient diagnosed with NF on the basis of the results of genetic testing who presented with early-onset wet cough and bronchiectasis. Considering the differential diagnosis of bronchiectasis combined with his early-onset wet cough, sinusitis, and sperm quality decline, we considered the possibility of primary ciliary dyskinesia (PCD). Further electron microscopy analysis of cilia and identification of homozygous mutations in the RSPH4A gene confirmed the diagnosis of PCD. Therefore, for patients with NF, when an image change exists in the lungs that does not correspond to NF, the possibility of other diagnoses, including PCD, must be considered.

关键词： primary ciliary dyskinesia neurofibromatosis bronchiectasis transmission electron microscopy genetic sequencing

HTML PDF 收藏

Experimental study on capturing CO 2 greenhouse gas by mixture of ammonia and soil

Ying WU, Yifei WANG, Qinghua ZENG, Xin GONG, Zunhong YU,

《化学科学与工程前沿（英文）》 2009年第3卷第4期页码 468-473 doi: 10.1007/s11705-009-0257-7

摘要： This paper presents our study on removal of carbon dioxide (CO) greenhouse gas emissions by using the mixture of ammonia and soil. CO capture capacity using this method is 15% higher than the sum of ammonia chemical absorption capacity and soil physical adsorption capacity. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) are utilized to study this synergism. The removal effect is not only reflected in ammonia chemical reaction with CO. CO can also be absorbed by ammonium bicarbonate (NHHCO) crystal, which is the main component of the product, or wrapped in the pore of the crystal or packed in the gap between the crystal and the soil. CO can be permanently deposited as carbonated minerals in the subsoil earth layers.

关键词： electron microscopy product physical adsorption capacity spectroscopy

HTML PDF 收藏

木质素磺酸盐/二氧化硅无机颗粒体系中的原子力显微镜力学测试——从分散机理研究到产品设计 Article

王静禹, 钱勇, 周义杰, 杨东杰, 邱学青

《工程（英文）》 2021年第7卷第8期页码 1140-1148 doi: 10.1016/j.eng.2021.07.004

摘要：

设计和制备高性能木质素基分散剂是实现木质素工程化高值利用的关键。这一过程很大程度上取决于对木质素基分散剂分散机理的理解。本文通过原子力显微镜（AFM）量化研究了木质素磺酸盐/二氧化硅（LS/SiO₂）体系在不同pH条件下的分散机理。结果表明，SiO₂颗粒之间在LS溶液中比在水中有更强的排斥力，因此具有更好的分散稳定性。分别使用Derjaguin-Landau-Verwey-Overbeek (DLVO)理论以及结合空间位阻排斥力的DLVO理论方程对水中和LS溶液中的SiO₂探针与基材之间的AFM力/距离曲线（F/D）进行拟合。基于拟合结果，分别计算出静电排斥力和空间位阻排斥力，证明LS在SiO₂粒子之间提供了较强的空间位阻排斥力。进一步研究证明，LS在SiO₂上的吸附量（Q）、归一化作用常数（A）和特征长度（L）是影响LS/SiO₂体系空间位阻排斥力的三个关键因素。基于上述研究，设计并制备了新型季铵化接枝磺化木质素基分散剂（QAGSL）。QAGSL对SiO₂和真实水泥颗粒均具有良好的分散性能。相关研究既为LS/无机颗粒体系中的分散机理提供了基础和定量的理解，也为高性能木质素基分散剂的开发提供了重要的技术指导。

关键词：木质素二氧化硅原子力显微镜分散机理产品设计