资源类型

期刊论文 21

年份

2024 1

2023 2

2022 4

2021 2

2020 2

2018 1

2017 2

2016 1

2015 1

2014 2

2010 1

展开 ︾

关键词

Cas12a 1

光 1

光形态建成 1

克罗诺杆菌 1

多黏菌素 1

大肠埃希菌 1

抗血清 1

核酸侧流免疫分析 1

检测方法 1

毒力 1

毒力因子 1

水产养殖 1

灰葡萄孢菌 1

环丙烷脂肪酰基磷脂合成酶 1

田间检测 1

疫苗 1

病原菌 1

稻瘟菌小麦致病型 1

细菌性病害 1

展开 ︾

检索范围：

排序： 展示方式：

New insights in the battle between wheat and

Chunlei TANG,Xiaojie WANG,Yulin CHENG,Minjie LIU,Mengxin ZHAO,Jinping WEI,Zhensheng KANG

《农业科学与工程前沿（英文）》 2015年 第2卷 第2期   页码 101-114 doi: 10.15302/J-FASE-2015068

摘要： Wheat stripe rust caused by f. sp. ( ) poses a great threat to wheat production worldwide. The rapid change in virulence of leads to a loss of resistance in currently resistant wheat cultivars, which results in frequent disease epidemics. Therefore, a major focus is currently placed on investigating the molecular mechanisms underlying this rapid variation of pathogenicity and coevolving wheat resistance. Limited by the lack of a system for stable transformation of and the difficulties in wheat transformation, it is not easy to generate deeper insights into the wheat- interaction using established genetic methods. Nevertheless, considerable effort has been made to unravel the wheat- interaction and significant progress is being made. Histology and cytology have revealed basic details of infection strategies and defense responses during wheat- interactions, identified cellular components involved in wheat- interactions, and have helped to elucidate their role in the infection process or in plant defense responses. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat- pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance response and in virulence. Studies of wheat- interactions have now entered a new phase in which cellular and molecular approaches are being used. This review focuses on the cellular biology of wheat- interactions and integrates the emerging data from molecular analyses with the histocytological observations.

关键词： wheat     strip rust     Puccinia striiformis f. sp. tritici     host defense     pathogen virulence     biotrophic fungus    

HTML PDF 收藏

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 121-122 doi: 10.15302/J-FASE-2017142

HTML PDF 收藏

Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence

《环境科学与工程前沿（英文）》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1682-4

摘要：

● Abundance of MAGs carrying ARG-VF pairs unchanged in rivers after WWTP upgrade.

关键词： Wastewater treatment plant upgrade     Antibiotic resistance genes (ARGs)     Virulence factors (VFs)     Gene co-occurrence     Genome-centric analysis    

HTML PDF 收藏

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 48-58 doi: 10.15302/J-FASE-2016114

摘要： Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

关键词： Puccinia striiformis f. sp. tritici     selfing     heterozygosity     virulence inheritance    

HTML PDF 收藏

spp., foodborne pathogens threatening neonates and infants

Qiming CHEN, Yang ZHU, Zhen QIN, Yongjun QIU, Liming ZHAO

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 330-339 doi: 10.15302/J-FASE-2018208

摘要：

Cronobacter spp. (formerly Enterobacter sakazakii) are special foodborne pathogens. Cronobacter infection can cause necrotizing enterocolitis, sepsis and meningitis in all age groups, especially neonates and infants, with a high fatality of up to 80%, although the infection is rare. Outbreaks of Cronobacter infection are epidemiologically proven to be associated with contaminated powdered infant formula (PIF). Cronobacter spp. can resist dry environments and survive for a long period in food with low water activity. Therefore, Cronobacter spp. have become serious pathogens of neonates and infants, as well as in the dairy industry. In this review, we present the taxonomy, pathogenesis, resistance, detection and control of Cronobacter spp.

关键词： Cronobacter spp.     desiccation resistance     pathogen control     pathogen detection     powdered infant formula    

HTML PDF 收藏

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 102-112 doi: 10.15302/J-FASE-2016100

摘要： Plants are frequently affected by pathogen infections. To effectively defend against such infections, two major modes of innate immunity have evolved in plants; pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity. Although the molecular components as well as the corresponding pathways involved in these two processes have been identified, many aspects of the molecular mechanisms of the plant immune system remain elusive. Recently, the rapid development of omics techniques (e.g., genomics, proteomics and transcriptomics) has provided a great opportunity to explore plant–pathogen interactions from a systems perspective and studies on protein–protein interactions (PPIs) between plants and pathogens have been carried out and characterized at the network level. In this review, we introduce experimental and computational identification methods of PPIs, popular PPI network analysis approaches, and existing bioinformatics resources/tools related to PPIs. Then, we focus on reviewing the progress in genome-wide PPI networks related to plant–pathogen interactions, including pathogen-centric PPI networks, plant-centric PPI networks and interspecies PPI networks between plants and pathogens. We anticipate genome-wide PPI network analysis will provide a clearer understanding of plant–pathogen interactions and will offer some new opportunities for crop protection and improvement.

关键词： plant–pathogen interactions     systems biology     omics     plant immunity     protein–protein interaction     network    

HTML PDF 收藏

Extracellular Vesicles in Pathogenic Infection, Transmission, and Immunity

Junyao Xiong,Usama Ashraf,Jing Ye,Shengbo Cao,

《工程（英文）》 doi: 10.1016/j.eng.2024.06.011

摘要： Extracellular vesicles (EVs) are secreted by cells and widely exist in body fluids, serving as an essential vehicle of intercellular communication. In recent years, EVs have gained significant attention owing to their ability to carry nucleic acids, lipids, and proteins of host and pathogen origins. A distinct composition of EVs during pathogenic infection contributes to mediating pathogenic infection, transmission, and immunity to target cells. Therefore, studying the role of EVs in pathogenic infection is crucial for understanding pathogen-host interactions and developing new anti-pathogenic therapies. This review offers an overview of current knowledge of EVs in the context of infection, transmission, and immunity to pathogens. Harnessing EVs’ biology during pathogenic infection may lay a foundation for the mitigation of pathogenic infection and associated disease outcomes.

关键词： Extracellular vesicles     Pathogen     Infection     Transmission     Immunity    

HTML PDF 收藏

Locally enhanced electric field treatment (LEEFT) for water disinfection

Jianfeng Zhou, Ting Wang, Cecilia Yu, Xing Xie

《环境科学与工程前沿（英文）》 2020年 第14卷 第5期 doi: 10.1007/s11783-020-1253-x

摘要： • Nanowire-assisted LEEFT is applied for water disinfection with low voltages. • LEEFT inactivates bacteria by disrupting cell membrane through electroporation. • Multiple electrodes and device configurations have been developed for LEEFT. • The LEEFT is low-cost, highly efficient, and produces no DBPs. • The LEEFT can potentially be applicable for water disinfection at all scales. Water disinfection is a critical step in water and wastewater treatment. The most widely used chlorination suffers from the formation of carcinogenic disinfection by-products (DBPs) while alternative methods (e.g., UV, O3, and membrane filtration) are limited by microbial regrowth, no residual disinfectant, and high operation cost. Here, a nanowire-enabled disinfection method, locally enhanced electric field treatment (LEEFT), is introduced with advantages of no chemical addition, no DBP formation, low energy consumption, and efficient microbial inactivation. Attributed to the lightning rod effect, the electric field near the tip area of the nanowires on the electrode is significantly enhanced to inactivate microbes, even though a small external voltage (usually<5 V) is applied. In this review, after emphasizing the significance of water disinfection, the theory of the LEEFT is explained. Subsequently, the recent development of the LEEFT technology on electrode materials and device configurations are summarized. The disinfection performance is analyzed, with respect to the operating parameters, universality against different microorganisms, electrode durability, and energy consumption. The studies on the inactivation mechanisms during the LEEFT are also reviewed. Lastly, the challenges and future research of LEEFT disinfection are discussed.

关键词： Water treatment     Nanotechnology     Pathogen inactivation     Electroporation     Nanowire     Chemical-free    

HTML PDF 收藏

临床上重要的革兰阴性菌多黏菌素耐药机制对毒力和适应性的影响 Review

王媛, 罗绮霞, 肖婷婷, 朱云颖, 肖永红

《工程（英文）》 2022年 第13卷 第6期   页码 178-185 doi: 10.1016/j.eng.2020.11.005

摘要：

随着多重耐药革兰阴性菌（如鲍曼不动杆菌、肺炎克雷伯菌和大肠埃希菌等）的出现，人类正在面临着巨大且日益严重的全球威胁。多黏菌素B和E（黏菌素）是治疗多重耐药革兰阴性菌的最后一线药物。多黏菌素是一种阳离子抗菌肽，能破坏革兰阴性菌的外膜。然而，随着多黏菌素临床应用的日益增多，有关多黏菌素耐药革兰阴性菌的报道也越来越多。多黏菌素耐药机制主要是通过脂多糖（lipopolysaccharide, LPS）的修饰或完全丧失介导的。LPS也是革兰阴性菌的毒力因子，LPS的改变可能与细菌的毒力有关。尽管人们普遍认为获得耐药性会使细菌产生适应性代价，当抗生素选择压力降低时，与耐药相关的适应性代价可能使耐药菌难以与敏感菌竞争，从而很快从群体中被清除。但如果菌株在获得耐药性的同时，适应性和毒力增强将会导致耐药菌株的广泛传播，造成巨大的临床损失。目前一些研究发现，与敏感菌相比，多黏菌素耐药菌具有更高的毒力和更强的适应性。为了预测多黏菌素耐药性的发展并评估缓解多黏菌素耐药性的干预措施，了解多黏菌素耐药菌株与敏感菌株之间的相对生物成本，本文将总结阐述多黏菌素耐药机制对鲍曼不动杆菌、肺炎克雷伯菌和大肠埃希菌毒力和适应性的影响。

关键词： 多黏菌素     耐药     毒力     适应性     革兰阴性菌     鲍曼不动杆菌     大肠埃希菌     肺炎克雷伯菌    

HTML PDF 收藏

PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing datasets as illustrated by application to COVID-19

《医学前沿（英文）》 2022年 第16卷 第2期   页码 251-262 doi: 10.1007/s11684-021-0915-9

摘要： Pathogenic microbes can induce cellular dysfunction, immune response, and cause infectious disease and other diseases including cancers. However, the cellular distributions of pathogens and their impact on host cells remain rarely explored due to the limited methods. Taking advantage of single-cell RNA-sequencing (scRNA-seq) analysis, we can assess the transcriptomic features at the single-cell level. Still, the tools used to interpret pathogens (such as viruses, bacteria, and fungi) at the single-cell level remain to be explored. Here, we introduced PathogenTrack, a python-based computational pipeline that uses unmapped scRNA-seq data to identify intracellular pathogens at the single-cell level. In addition, we established an R package named Yeskit to import, integrate, analyze, and interpret pathogen abundance and transcriptomic features in host cells. Robustness of these tools has been tested on various real and simulated scRNA-seq datasets. PathogenTrack is competitive to the state-of-the-art tools such as Viral-Track, and the first tools for identifying bacteria at the single-cell level. Using the raw data of bronchoalveolar lavage fluid samples (BALF) from COVID-19 patients in the SRA database, we found the SARS-CoV-2 virus exists in multiple cell types including epithelial cells and macrophages. SARS-CoV-2-positive neutrophils showed increased expression of genes related to type I interferon pathway and antigen presenting module. Additionally, we observed the Haemophilus parahaemolyticus in some macrophage and epithelial cells, indicating a co-infection of the bacterium in some severe cases of COVID-19. The PathogenTrack pipeline and the Yeskit package are publicly available at GitHub.

关键词： scRNA-seq     intracellular pathogen     microbe     COVID-19     SARS-CoV-2    

HTML PDF 收藏

光响应基因BcCfaS通过脂质代谢调控灰葡萄孢菌的光形态建成及致病力 Article

李广锦, 张占全, 陈勇, 陈彤, 李博强, 田世平​​​​​​​

《工程（英文）》 2024年 第35卷 第4期   页码 201-213 doi: 10.1016/j.eng.2023.08.015

摘要：

光是地球上生命赖以生存所必需的环境因子，它不仅是能量来源，也是一种信号分子。真菌能通过光获取时间和空间信息，进而控制重要的生理和形态应答。Botrytis cinerea是一种危害性极大的植物病原真菌，它能利用光响应来优化致病能力，通过平衡产孢量和菌核发育，实现在不同环境中的传播和生存。然而，有关B. cinerea光信号转导组分及调控机制至今尚不清楚。在本研究中，我们从B. cinerea中鉴定到一个光信号转导途径的新组分BcCfaS，该基因编码一个环丙烷脂肪酰基磷脂合成酶。BcCfaS在转录水平上受光的显著诱导，并在光形态建成中发挥重要的调控作用。BcCfaS缺失可导致营养生长缓慢，菌落形态改变，产核减少和产孢增加，其变化与光照条件密切相关。此外，该突变体在逆境应答和对寄主的致病力方面表现出严重缺陷。基于脂质代谢组学分析，我们鉴定到了许多未知的且受BcCfaS调控的脂质，包括一些磷脂和脂肪酸。研究表明，BcCfaS通过正调控茉莉酸甲酯合成，在转录水平激活光信号组分基因表达，从而控制产孢和菌核发育，这些研究结果为深入认知真菌光形态建成的物质代谢基础提供了新证据。我们认为BcCfaS是整合光信号和脂质代谢的关键节点，揭示了真菌通过调节发育模式来应对光环境变化的协同机制。这些新发现为创制真菌病害防控策略提供了重要的靶点。

关键词： 光     灰葡萄孢菌     环丙烷脂肪酰基磷脂合成酶     脂质代谢     光形态建成     致病力    

HTML PDF 收藏

Clinical manifestations and pathogen characteristics in children admitted for suspected COVID-19

Xiaofang Cai, Hanlan Jiang, Simin Zhang, Shengying Xia, Wenhui Du, Yaoling Ma, Tao Yu, Wenbin Li

《医学前沿（英文）》 2020年 第14卷 第6期   页码 776-785 doi: 10.1007/s11684-020-0820-7

摘要： Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has spread around the world. However, approaches to distinguish COVID-19 from pneumonia caused by other pathogens have not yet been reported. We retrospectively analyzed the clinical data of 97 children with probable COVID-19. A total of 13 (13.4%) patients were confirmed positive for SARS-CoV-2 infection by nucleic acid RT-PCR testing, and 41 (42.3%) patients were found to be infected with other pathogens. Notably, no pathogen was detected in 43 (44.3%) patients. Among all patients, 25 (25.8%) had familial cluster exposure history, and 52 (53.6%) had one or more coexisting conditions. Fifteen (15.5%) patients were admitted or transferred to the PICU. In the 11 confirmed COVID-19 cases, 5 (45.5%) and 7 (63.6%) were positive for IgM and IgG against SARS-CoV-2, respectively. In 22 patients with suspected COVID-19, 1 (4.5%) was positive for IgG but negative for IgM. The most frequently detected pathogen was (29, 29.9%). One patient with confirmed COVID 19 died. Our results strongly indicated that the detection of asymptomatic COVID 19 or coexisting conditions must be strengthened in pediatric patients. These cases may be difficult to diagnose as COVID-19 unless etiologic analysis is conducted. A serologic test can be a useful adjunctive diagnostic tool in cases where SARS-CoV-2 infection is highly suspected but the nucleic acid test is negative.

关键词： coronavirus disease 2019     pediatrics     emergency     retrospective investigation     severe acute respiratory syndrome coronavirus 2    

HTML PDF 收藏

Composition, dispersion, and health risks of bioaerosols in wastewater treatment plants: A review

Yunping Han, Lin Li, Ying Wang, Jiawei Ma, Pengyu Li, Chao Han, Junxin Liu

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1330-1

摘要： Abstract • Bioaerosols are produced in the process of wastewater biological treatment. • The concentration of bioaerosol indoor is higher than outdoor. • Bioaerosols contain large amounts of potentially pathogenic biomass and chemicals. • Inhalation is the main route of exposure of bioaerosol. • Both the workers and the surrounding residents will be affected by the bioaerosol. Bioaerosols are defined as airborne particles (0.05–100 mm in size) of biological origin. They are considered potentially harmful to human health as they can contain pathogens such as bacteria, fungi, and viruses. This review summarizes the most recent research on the health risks of bioaerosols emitted from wastewater treatment plants (WWTPs) in order to improve the control of such bioaerosols. The concentration and size distribution of WWTP bioaerosols; their major emission sources, composition, and health risks; and considerations for future research are discussed. The major themes and findings in the literature are as follows: the major emission sources of WWTP bioaerosols include screen rooms, sludge-dewatering rooms, and aeration tanks; the bioaerosol concentrations in screen and sludge-dewatering rooms are higher than those outdoors. WWTP bioaerosols contain a variety of potentially pathogenic bacteria, fungi, antibiotic resistance genes, viruses, endotoxins, and toxic metal(loid)s. These potentially pathogenic substances spread with the bioaerosols, thereby posing health risks to workers and residents in and around the WWTP. Inhalation has been identified as the main exposure route, and children are at a higher risk of this than adults. Future studies should identify emerging contaminants, establish health risk assessments, and develop prevention and control systems.

关键词： Wastewater treatment plant     Bioaerosols     Pathogen     Dispersion     Risk assessment    

HTML PDF 收藏

Pathogen evolution, prevention/control strategy and clinical features of COVID-19: experiences from China

《医学前沿（英文）》 2023年 第17卷 第6期   页码 1030-1046 doi: 10.1007/s11684-023-1043-5

摘要： Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported at the end of 2019 as a worldwide health concern causing a pandemic of unusual viral pneumonia and many other organ damages, which was defined by the World Health Organization as coronavirus disease 2019 (COVID-19). The pandemic is considered a significant threat to global public health till now. In this review, we have summarized the lessons learnt during the emergence and spread of SARS-CoV-2, including its prototype and variants. The overall clinical features of variants of concern (VOC), heterogeneity in the clinical manifestations, radiology and pathology of COVID-19 patients are also discussed, along with advances in therapeutic agents.

关键词： coronavirus disease 2019     SARS-CoV-2     epidemiology     clinical features    

HTML PDF 收藏

Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes in reclaimed water

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1521-z

摘要：

• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.

关键词： UV/chlorine process     Pathogen     Antibiotic resistance genes     High-throughput qPCR     Reclaimed water    

HTML PDF 收藏

标题 作者 时间 类型 操作

New insights in the battle between wheat and

Chunlei TANG,Xiaojie WANG,Yulin CHENG,Minjie LIU,Mengxin ZHAO,Jinping WEI,Zhensheng KANG

期刊论文

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

期刊论文

Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence

期刊论文

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

期刊论文

spp., foodborne pathogens threatening neonates and infants

Qiming CHEN, Yang ZHU, Zhen QIN, Yongjun QIU, Liming ZHAO

期刊论文

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

期刊论文

Extracellular Vesicles in Pathogenic Infection, Transmission, and Immunity

Junyao Xiong,Usama Ashraf,Jing Ye,Shengbo Cao,

期刊论文

Locally enhanced electric field treatment (LEEFT) for water disinfection

Jianfeng Zhou, Ting Wang, Cecilia Yu, Xing Xie

期刊论文

临床上重要的革兰阴性菌多黏菌素耐药机制对毒力和适应性的影响

王媛, 罗绮霞, 肖婷婷, 朱云颖, 肖永红

期刊论文

PathogenTrack and Yeskit: tools for identifying intracellular pathogens from single-cell RNA-sequencing datasets as illustrated by application to COVID-19

期刊论文

光响应基因BcCfaS通过脂质代谢调控灰葡萄孢菌的光形态建成及致病力

李广锦, 张占全, 陈勇, 陈彤, 李博强, 田世平​​​​​​​

期刊论文

Clinical manifestations and pathogen characteristics in children admitted for suspected COVID-19

Xiaofang Cai, Hanlan Jiang, Simin Zhang, Shengying Xia, Wenhui Du, Yaoling Ma, Tao Yu, Wenbin Li

期刊论文

Composition, dispersion, and health risks of bioaerosols in wastewater treatment plants: A review

Yunping Han, Lin Li, Ying Wang, Jiawei Ma, Pengyu Li, Chao Han, Junxin Liu

期刊论文

Pathogen evolution, prevention/control strategy and clinical features of COVID-19: experiences from China

期刊论文

Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes in reclaimed water

期刊论文