检索范围：
排序： 展示方式：
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
《化学科学与工程前沿（英文）》 2018年 第12卷 第4期 页码 806-814 doi: 10.1007/s11705-018-1739-2
The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.
关键词： synthetic biology genome rearrangement prodeoxyviolacein SCRaMbLE Saccharomyces cerevisiae
标题 作者 时间 类型 操作
京公网安备 11010502051620号
