检索范围:
排序: 展示方式:
c-Fos expression in rat brainstem following intake of sucrose or saccharin
Ke Chen, Jianqun Yan, Jinrong Li, Bo Lv, Xiaolin Zhao
《医学前沿(英文)》 2011年 第5卷 第3期 页码 294-301 doi: 10.1007/s11684-011-0144-8
关键词: c-Fos parabrachial the nucleus of the solitary tract sweet tastant rat
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 362-372 doi: 10.15302/J-FASE-2018206
This study compared proteome profiles and morphological changes of rat jejunum in response to different dietary proteins. Fifty male Sprague-Dawley rats were fed with casein (control), and isolated beef, pork, fish and chicken proteins for 14 days. Proteome analysis, histological observation and PEPT1 quantification of the jejunum were performed. The results indicated that rats fed with chicken proteins had higher PEPT1 mRNA and protein levels (P<0.05) but lower villus height and ratio of villus height to crypt depth (V/C ratio, P<0.05) than those fed with casein and pork protein. Label-free LC-MS/MS indicated that, as compared to casein, intake of chicken protein can regulate oligopeptide transport mainly by upregulating PEPT1 protein expression and reducing dipeptidyl-peptidase activity related to biological oxidation, and can reduce oligopeptide absorption capacity by regulating Hippo signaling pathway. Although intake of beef and fish proteins had no significant effect on PEPT1 expression, they altered several signaling pathways.
关键词: Hippo signaling pathway meat protein PEPT1 proteome analysis rat jejunum
《医学前沿(英文)》 2022年 第16卷 第4期 页码 584-595 doi: 10.1007/s11684-021-0844-7
关键词: hemophilia A adeno-associated virus (AAV) human/rat hybrid factor VIII gene therapy dual chain strategy
A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 367-377 doi: 10.1007/s11684-013-0266-2
This study aimed to modify a chronic ocular hypertension (OHT) rat model to screen for potential compounds to protect retinal ganglion cells (RGCs) from responding to increased intraocular pressure (IOP). A total of 266 rats were prepared and randomly grouped according to different time-points, namely, weeks 3, 8, 16, and 24. Rats were sedated and eye examination was performed to score as the corneal damage on a scale of 1 to 4. The OHT rat model was created via the injection of a hypertonic saline solution into the episcleral veins once weekly for two weeks. OHT was identified when the IOP at week 0 was≥6 mmHg than that at week -2 for the same eye. Viable RGCs were labeled by injecting 4% FluoroGold. Rats were sacrificed, and the eyes were enucleated and fixed. The fixed retinas were dissected to prepare flat whole-mounts. The viable RGCs were visualized and imaged. The IOP (meanβ±βSD) was calculated, and data were analyzed by the paired t-test and one-way ANOVA. The OHT model was created in 234 of 266 rats (87.97%), whereas 32 rats (12.03%) were removed from the study because of the absence of IOP elevation (11.28%) and/or corneal damage scores over 4 (0.75%). IOP was elevated by as much as 81.35% for 24 weeks. The average IOP was (16.68β±β0.98)βmmHg in non-OHT eyes (n = 234), but was (27.95±0.97)βmmHg in OHT eyes (n = 234). Viable RGCs in the OHT eyes were significantly decreased in a time-dependent manner by 29.41%, 38.24%, 55.32%, and 59.30% at weeks 3, 8, 16, and 24, respectively, as compared to viable RGCs in the non-OHT eyes (P<β0.05). The OHT model was successfully created in 88% of the rats. The IOP in the OHT eyes was elevated by approximately 81% for 24 weeks. The number of viable RGCs was decreased by 59% of the rats in a time-dependent manner. The modified OHT model may provide an effective and reliable method for screening drugs to protect RGCs from glaucoma.
关键词: chronic ocular hypertension intraocular pressure retinal ganglion cells neuroprotection glaucoma
XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai
《医学前沿(英文)》 2008年 第2卷 第3期 页码 264-268 doi: 10.1007/s11684-008-0050-x
关键词: resistance infrared infrared videomicroscopy depressive after-hyperpolarization potential
Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene
Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 57-60 doi: 10.1007/s11684-009-0003-z
Zhao DING, Zhishui CHEN, Xilin CHEN, Ming CAI, Hui GUO, Nianqiao GONG
《医学前沿(英文)》 2009年 第3卷 第2期 页码 204-210 doi: 10.1007/s11684-009-0039-0
关键词: anti-ERK2 renal transplantation epithelial mesenchymal transition chronic allograft nephropathy
Protein aggregation in association with delayed neuronal death in rat model of brain ischemia
GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei
《医学前沿(英文)》 2008年 第2卷 第1期 页码 70-74 doi: 10.1007/s11684-008-0013-2
null
《医学前沿(英文)》 2012年 第6卷 第4期 页码 411-415 doi: 10.1007/s11684-012-0226-2
Ketamine exerts rapid and robust antidepressant properties in both animal models and depressed patients and tramadol possesses potential antidepressant effects. Brain-derived neurotrophic factor (BDNF) is an important biomarker for mood disorders and tropomyosin-related kinase B (TrkB) is a high affinity catalytic receptor for BDNF. We hypothesized that tramadol pretreatment might reinforce ketamine-elicited antidepressant effects with significant changes in hippocampal BDNF and TrkB levels in rats. Immobility time of rats receiving different treatment in the forced swimming test (FST) was observed. Levels of BDNF and TrkB in hippocampus were measured by enzyme linked immunosorbent assay. Results showed that tramadol (5 mg/kg) administrated alone neither elicited antidepressant effects nor altered BDNF or TrkB level. However, pretreatment with tramadol (5 mg/kg) enhanced the ketamine (10 mg/kg) -elicited antidepressant effects and upregulated the BDNF and TrkB levels in hippocampus. In conclusion, tramadol pretreatment reinforces the ketamine-elicited antidepressant effects, which is associated with the increased levels of BDNF and TrkB in rat hippocampus.
关键词: tramadol ketamine antidepressant brain-derived neurotrophic factor tropomyosin-related kinase B
null
《医学前沿(英文)》 2016年 第10卷 第3期 页码 286-296 doi: 10.1007/s11684-016-0456-9
Exogenic electric fields can effectively accelerate bone healing and remodeling through the enhanced migration of bone marrow mesenchymal stem cells (BMSCs) toward the injured area. This study aimed to determine the following: (1) the direction of rat BMSC (rBMSC) migration upon exposure to a direct current electric field (DCEF), (2) the optimal DCEF intensity and duration, and (3) the possible regulatory role of SDF-1/CXCR4 axis in rBMSC migration as induced by DCEF. Results showed that rBMSCs migrated to the positive electrode of the DCEF, and that the DCEF of 200 mV/mm for 4 h was found to be optimal in enhancing rBMSC migration. This DCEF strength and duration also upregulated the expression of osteoblastic genes, including ALP and OCN, and upregulated the expression of ALP and Runx2 proteins. Moreover, when CXCR4 was inhibited, rBMSC migration due to DCEF was partially blocked. These findings indicated that DCEF can effectively induce rBMSC migration. A DCEF of 200 mV/mm for 4 h was recommended because of its ability to promote rBMSC migration, proliferation, and osteogenic differentiation. The SDF-1/CXCR4 signaling pathway may play an important role in regulating the DCEF-induced migration of rBMSCs.
关键词: DCEF migration osteogenesis differentiation rBMSCs SDF-1/CXCR-4
Mechanism of hepatocellular damage in rat caused by low serum selenium
LU Yi, QU Bo, LIU Chang, YU Liang, Liu Xuemin, WANG Haohua, JIANG An, ZHANG Xiaogang
《医学前沿(英文)》 2008年 第2卷 第3期 页码 255-258 doi: 10.1007/s11684-008-0048-4
关键词: process glutathione peroxidase hepatocellular damage group Keshan Disease
Xiao He, Chentao Jin, Mindi Ma, Rui Zhou, Shuang Wu, Haoying Huang, Yuting Li, Qiaozhen Chen, Mingrong Zhang, Hong Zhang, Mei Tian
《医学前沿(英文)》 2019年 第13卷 第5期 页码 602-609 doi: 10.1007/s11684-019-0704-x
关键词: panic disorder (PD) positron emission tomography (PET) optogenetics dorsal periaqueductal gray (dPAG)
Effects of hypoxia on mRNA expression of housekeeping genes in rat brain tissue and primary cultured
YANG Yingzhong, MA Lan, GE Rili, FAN Wenhong, ZHU Lingling, ZHAO Tong, WU Yan, FAN Ming
《医学前沿(英文)》 2008年 第2卷 第3期 页码 239-243 doi: 10.1007/s11684-008-0045-7
关键词: Glyceraldehyde-3-phosphate dehydrogenase comparative constant unfavorable independent
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 97-109 doi: 10.1007/s11684-016-0496-1
As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages. Isobaric tag for relative and absolute quantitation-based 2D nano-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight (MALDI-TOF/TOF) technologies were used. Global protein profiling, KEGG and PANTHER pathways, and functional categories were analyzed. Consequently, histological and tartrate-resistant acid phosphatase staining indicated the altered histological structure of condylar cartilage and increased bone remodeling activity in hard-diet group. A total of 805 differentially expressed proteins were then identified. GO analysis revealed a significant number of proteins involved in the metabolic process, cellular process, biological regulation, localization, developmental process, and response to stimulus. KEGG pathway analysis also suggested that these proteins participated in various signaling pathways, including calcium signaling pathway, gap junction, ErbB signaling pathway, and mitogen-activated protein kinase signaling pathway. Collagen types I and II were further validated by immunohistochemical staining and Western blot analysis. Taken together, the present study provides an insight into the molecular mechanism of regulating condylar growth and remodeling induced by reducing dietary loading at the protein level.
关键词: condylar cartilage mechanical loading proteomic analysis iTRAQ bioinformatics analysis
张荣利,冯 雪,张会亮,罗国安,李连达,王义明
《中国工程科学》 2007年 第9卷 第7期 页码 89-94
采用密度梯度离心法分离骨髓间质干细胞,差速贴壁法进行纯化,应用流式细胞仪、激光共聚焦显微镜等对纯化细胞进行鉴定,结果细胞表面抗原CD29,CD44,CD105,CD166表达呈阳性,而CD14,CD34,CD45表达呈阴性。采用RT唱PCR鉴定了三个基因:nestin,NST,Oct-4,前两者呈阳性表达,后者弱阳性表达。这些细胞特异抗原与基因表达综合起来,表明得到的细胞具备骨髓间质干细胞的特性。密度梯度分离与差速贴壁相结合,可获得较好均一性的骨髓间质干细胞,是一种简单可靠、易于推广的骨髓干细胞获取方法,可为细胞与组织工程研究提供种子细胞。
标题 作者 时间 类型 操作
c-Fos expression in rat brainstem following intake of sucrose or saccharin
Ke Chen, Jianqun Yan, Jinrong Li, Bo Lv, Xiaolin Zhao
期刊论文
Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum
Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU
期刊论文
diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed of the human heavy chain and the rat
期刊论文
A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection
null
期刊论文
Spontaneous firing properties of rat medial vestibular nucleus neurons in brain slices by infrared visual
XIA Jiao, KONG Weijia, ZHU Yun, ZHOU Yan, ZHANG Yu, GUO Changkai
期刊论文
Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene
Zhengjuan LIU, Jie BIAN, Yuchuan WANG, Yongli ZHAO, Dong YAN, Xiaoxia WANG
期刊论文
Adenovirus-mediated antisense ERK2 gene therapy ameliorates chronic allograft nephropathy in a rat model
Zhao DING, Zhishui CHEN, Xilin CHEN, Ming CAI, Hui GUO, Nianqiao GONG
期刊论文
Protein aggregation in association with delayed neuronal death in rat model of brain ischemia
GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei
期刊论文
ketamine with increased levels of brain-derived neurotrophic factor and tropomyosin-related kinase B in rat
null
期刊论文
of the intensity and loading time of direct current electric field on the directional migration of rat
null
期刊论文
Mechanism of hepatocellular damage in rat caused by low serum selenium
LU Yi, QU Bo, LIU Chang, YU Liang, Liu Xuemin, WANG Haohua, JIANG An, ZHANG Xiaogang
期刊论文
PET imaging on neurofunctional changes after optogenetic stimulation in a rat model of panic disorder
Xiao He, Chentao Jin, Mindi Ma, Rui Zhou, Shuang Wu, Haoying Huang, Yuting Li, Qiaozhen Chen, Mingrong Zhang, Hong Zhang, Mei Tian
期刊论文
Effects of hypoxia on mRNA expression of housekeeping genes in rat brain tissue and primary cultured
YANG Yingzhong, MA Lan, GE Rili, FAN Wenhong, ZHU Lingling, ZHAO Tong, WU Yan, FAN Ming
期刊论文
iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar
null
期刊论文