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制药工业 1

土壤施用 1

抗生素 1

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The antibiotic resistome: gene flow in environments, animals and human beings

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《医学前沿(英文)》 2017年 第11卷 第2期   页码 161-168 doi: 10.1007/s11684-017-0531-x

摘要:

The antibiotic resistance is natural in bacteria and predates the human use of antibiotics. Numerous antibiotic resistance genes (ARGs) have been discovered to confer resistance to a wide range of antibiotics. The ARGs in natural environments are highly integrated and tightly regulated in specific bacterial metabolic networks. However, the antibiotic selection pressure conferred by the use of antibiotics in both human medicine and agriculture practice leads to a significant increase of antibiotic resistance and a steady accumulation of ARGs in bacteria. In this review, we summarized, with an emphasis on an ecological point of view, the important research progress regarding the collective ARGs (antibiotic resistome) in bacterial communities of natural environments, human and animals, i.e., in the one health settings. We propose that the resistance gene flow in nature is “from the natural environments” and “to the natural environments”; human and animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene “circulation.”

关键词: antibiotic resistance     resistome     microbiome     gene flow    

Antibiotic resistome of : molecular determinants for the emergence of drug resistance

《医学前沿(英文)》 2021年 第15卷 第5期   页码 693-703 doi: 10.1007/s11684-020-0777-6

摘要: Resistome is a cluster of microbial genes encoding proteins with necessary functions to resist the action of antibiotics. Resistome governs essential and separate biological functions to develop resistance against antibiotics. The widespread clinical and nonclinical uses of antibiotics over the years have combined to select antibiotic-resistant determinants and develop resistome in bacteria. At present, the emergence of drug resistance because of resistome is a significant problem faced by clinicians for the treatment of Salmonella infection. Antibiotic resistome is a dynamic and ever-expanding component in Salmonella. The foundation of resistome in Salmonella is laid long before; therefore, the antibiotic resistome of Salmonella is reviewed, discussed, and summarized. We have searched the literature using PubMed, MEDLINE, and Google Scholar with related key terms (resistome, Salmonella, antibiotics, drug resistance) and prepared this review. In this review, we summarize the status of resistance against antibiotics in S. typhi, highlight the seminal work in the resistome of S. typhi and the genes involved in the antibiotic resistance, and discuss the various methods to identify S. typhi resistome for the proactive identification of this infection and quick diagnosis of the disease.

关键词: S. typhi     antibiotic resistance     mechanism     resistome     identification methods    

Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain

《环境科学与工程前沿(英文)》 2022年 第16卷 第3期 doi: 10.1007/s11783-021-1466-7

摘要:

• The α-diversities of resistome were lower in manure and compost than in soils.

关键词: Antibiotic resistance genes     Resistome     Bacterial taxonomy     Transmission chain    

Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review

Kun Wan, Wenfang Lin, Shuai Zhu, Shenghua Zhang, Xin Yu

《环境科学与工程前沿(英文)》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1189-1

摘要: Published data was used to analyze the fate of ARGs in water treatment. Biomass removal leads to the reduction in absolute abundance of ARGs. Mechanism that filter biofilm maintain ARB/ARGs was summarized. Potential BAR risks caused by biofiltration and chlorination were proposed. The bacterial antibiotic resistome (BAR) is one of the most serious contemporary medical challenges. The BAR problem in drinking water is receiving growing attention. In this study, we focused on the distribution, changes, and health risks of the BAR throughout the drinking water treatment system. We extracted the antibiotic resistance gene (ARG) data from recent publications and analyzed ARG profiles based on diversity, absolute abundance, and relative abundance. The absolute abundance of ARG was found to decrease with water treatment processes and was positively correlated with the abundance of 16S rRNA (r2 = 0.963, p<0.001), indicating that the reduction of ARG concentration was accompanied by decreasing biomass. Among treatment processes, biofiltration and chlorination were discovered to play important roles in shaping the bacterial antibiotic resistome. Chlorination exhibited positive effects in controlling the diversity of ARG, while biofiltration, especially granular activated carbon filtration, increased the diversity of ARG. Both biofiltration and chlorination altered the structure of the resistome by affecting relative ARG abundance. In addition, we analyzed the mechanism behind the impact of biofiltration and chlorination on the bacterial antibiotic resistome. By intercepting influent ARG-carrying bacteria, biofilters can enrich various ARGs and maintain ARGs in biofilm. Chlorination further selects bacteria co-resistant to chlorine and antibiotics. Finally, we proposed the BAR health risks caused by biofiltration and chlorination in water treatment. To reduce potential BAR risk in drinking water, membrane filtration technology and water boiling are recommended at the point of use.

关键词: Drinking water treatment     Antibiotic resistance gene     Biofiltration     Chlorination    

Culturomics and metagenomics: In understanding of environmental resistome

Monika Nowrotek, Łukasz Jałowiecki, Monika Harnisz, Grażyna Anna Płaza

《环境科学与工程前沿(英文)》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1121-8

摘要:

State of the art of culturomics and metagenomics to study resistome was presented.

The combination of culturomics and metagenomics approaches was proposed.

The research directions of antibiotic resistance study has been suggested.

关键词: Culturomics     Metagenomics     Antibiotic resistance     Resistome    

红霉素发酵菌渣长期施用对土壤耐药基因的累积影响 Article

韩子铭, 冯皓迪, 栾晓, 沈云鹏, 任立人, 邓留杰, D.G. Joakim Larsson, Michael Gillings, 张昱, 杨敏​​​​​​​

《工程(英文)》 2022年 第15卷 第8期   页码 78-88 doi: 10.1016/j.eng.2022.05.011

摘要:

抗生素发酵生产过程产生的富含有机质的发酵菌渣具有资源化的潜力,但残留的高浓度抗生素限制了其资源化利用。本研究调研了一处工业规模的红霉素发酵菌渣(EFR)处理设施,并将一定条件下水热处理后的EFR作为有机肥,设置农业常规剂量和极端高剂量(3750~15 000 kg⋅hm−2),开展了连续三年的野外大田施用实验。水热处理有效地灭活了微生物,降解了DNA,红霉素的去除率达到约98%。菌渣有机肥的施用向土壤引入了持续三年的μg∙kg−1水平的亚抑制红霉素压力。土壤宏基因组测序发现,农业常规剂量施用对土壤耐药基因影响较弱,但极端高剂量施用对土壤耐药基因产生累积影响。具体而言,土壤耐药基因丰度和多样性没有受到单次(第一年)施用的影响,但在第二年和第三年期间逐渐发生变化,大环内酯类耐药基因被选择性富集。土壤细菌群落和可移动遗传元件的变化趋势与耐药基因相似,相关性分析和结构方程模型均表明三年期间红霉素、细菌群落、可移动遗传元件与耐药基因的关联性逐渐增强。被富集的大环内酯类耐药基因以RNA甲基化酶基因erm和外排泵基因mac为主;宏基因组组装与分箱结果表明,水平转移在erm基因的传播中发挥重要作用,mac基因的富集则主要受垂直传播的影响。本研究揭示了亚抑制水平红霉素长期暴露对土壤耐药基因产生累积影响的机制,菌渣中抗生素的高效去除和菌渣环境耐药性影响的长期评价对未来菌渣安全处置与资源化利用至关重要。

关键词: 制药工业     耐药基因组     抗生素     风险评估     修复     土壤施用    

标题 作者 时间 类型 操作

The antibiotic resistome: gene flow in environments, animals and human beings

null

期刊论文

Antibiotic resistome of : molecular determinants for the emergence of drug resistance

期刊论文

Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain

期刊论文

Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review

Kun Wan, Wenfang Lin, Shuai Zhu, Shenghua Zhang, Xin Yu

期刊论文

Culturomics and metagenomics: In understanding of environmental resistome

Monika Nowrotek, Łukasz Jałowiecki, Monika Harnisz, Grażyna Anna Płaza

期刊论文

红霉素发酵菌渣长期施用对土壤耐药基因的累积影响

韩子铭, 冯皓迪, 栾晓, 沈云鹏, 任立人, 邓留杰, D.G. Joakim Larsson, Michael Gillings, 张昱, 杨敏​​​​​​​

期刊论文