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High specificity detection of Pb

Ruozhen YU,Zheng FANG,Wei MENG,Zhenguang YAN,Lina DU,Hong WANG,Zhengtao LIU

《环境科学与工程前沿（英文）》 2014年第8卷第5期页码 729-736 doi: 10.1007/s11783-013-0611-3

摘要： Only one bifunctional metal-chelator was used to prepare immunogen and coating antigen in all of the previous researches. However, the antibody-specific recognition to the spacer arm of the bifunctional metal-chelator might lower the specificity of heavy metal ions immunoassay. Two different bifunctional metal-chelators were adopted to prepare the immunogen and coating antigen respectively in our study to avoid this problem. The conjugates of keyhole limpet hemocyanin (KLH) and -SCN-Bz-DTPA-Pb were used as immunogen, whereas the conjugates of bovine serum albumin (BSA) and -NH -Bn-DTPA-Pb were used as coating antigen. Polyclonal antibodies specific to DTPA-Pb chelates were obtained from rabbits. Indirect competitive enzyme-linked immunosorbent assay (ELISA) was adopted to detect Pb ion solutions prepared by Pb standard solution and ultrapure water. In the mixing microplate, DTPA and Pb ions formed chelates and combined with specific antibodies. After incubation, the DTPA-Pb and the antibodies complex were added into the wells of the reaction microplate. The reaction microplate was coated by the conjugates of BSA and -NH -DTPA-Pb, which competed for the specific antibodies. The result signals presented a good sigmoid curve when the Pb concentration ranges from 0.01 to 100 mg·L . The IC50 of the indirect competitive ELISA is 0.23±0.04 mg·L Pb ion. The cross-reaction with Cd , Cu , Fe , Mn , Zn , and other divalent ions were less than 5%.

关键词： p-SCN-Bz-DTPA p-NH₂-Bn-DTPA lead ion polyclonal antibody

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Meta-analysis of the diagnostic efficiency of frequency-doubling technology for primary glaucoma

LIU Ting, HE Xiangge

《医学前沿（英文）》 2007年第1卷第1期页码 109-114 doi: 10.1007/s11684-007-0021-7

摘要： The overall diagnostic capabilities and utility of frequency-doubling technology (FDT) in patients with primary glaucoma, which was diagnosed by standard automated perimetry (SAP) and/or optic disc appearance as the gold standard, were assessed. A comprehensive electric retrieval in MEDLINE, EMBASE, Cochrane Library, BIOSIS, Previews, HMIC, IPA, OVID, CNKI, CBMdisc, VIP information, CMCC, CCPD, SSreader, 21dmedia and manual retrieval in related textbooks, journals, congress articles and their references at home and abroad were performed to identify relevant articles in English or Chinese. The corresponding references were retrieved by means of electric retrieval and manual retrieval from different databases or materials. Criteria for inclusion or exclusion were established according to validity criteria for diagnostic studies published by the Cochrane Methods Group on Screening and Diagnostic Tests. The quality of the included articles was then assessed and characteristics were extracted. Statistical analysis was performed with Meta Test version 0.6 software to test the heterogeneity of the included articles. The appropriate effects model was selected to calculate pooled weighted sensitivity and specificity. Summary receiver operating characteristic (SROC) curve was drawn and the area under the curve (AUC) was calculated. Finally, sensitivity analysis was performed. Twenty-one studies out of 206 retrieved articles were included, with a total of 3172 patients. The reported sensitivity of FDT ranged from 0.51 to 1.00, and specificity from 0.58 to 1.00. The pooled weighted sensitivity and specificity of FDT with 95% confidence intervals (95% CI) after correction for standard error were 0.86 (0.80 0.90) and 0.87 (0.81 0.91), respectively. The AUC of SROC was 93.01%. Sensitivity analysis demonstrated no disproportionate influences of individual studies. The included articles are of good quality and FDT can be a highly efficient diagnostic test for primary glaucoma based on Meta-analysis.

关键词： inclusion sensitivity analysis correction specificity appropriate

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Screening of indicator pharmaceuticals and personal care products in landfill leachates: a case study in Shanghai, China

《环境科学与工程前沿（英文）》 2023年第17卷第9期 doi: 10.1007/s11783-023-1716-y

摘要：

● A systematic framework was developed to identify i-PPCPs for landfill leachate.

关键词： Landfill leachates PPCPs Indicator Screening criteria Source-specificity

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Construction of 6HRE-GFAP-Baxα system specific for glioma gene therapy

TIAN Yongji, LI Guilin, GAO Jun, WANG Renzhi, KONG Yanguo, ZHANG Zhenxing, LI Shifang, TIAN Shiqiang, DOU Wanchen, ZHANG Bo

《医学前沿（英文）》 2007年第1卷第1期页码 49-53 doi: 10.1007/s11684-007-0010-x

摘要： The aim of this paper is to construct a specific and high-performance gene therapy system for glioma. We constructed a combined promoter 6HRE-GFAP (Hypoxia Responsive Element, Glial Fibrillary Acidic Protein) by gene recombination techniques according to the hypoxia microenvironment in glioma and tested its efficacy and specificity in cultured cells, and then constructed GFAP-Baxα gene expressing system and determined its promoting of apoptosis in glioma cells. Our primary results showed that in U251 and BT325 cell lines, the activity of GFAP promoter was 16.40 and 4.73-fold of the promoter of hTERT (Human Telomerase Reverse Transcriptase), respectively. The activities of 6HRE-GFAP-promoter increased by 3.08 and 1.30-fold under 2% O condition compared with those under 18% O condition, while under 0.2% O condition increased by 8.90 and 2.69-fold, respectively. The glioma cells showed typical apoptotic signs 90 hours after the transient transfection of GFAP-Baxα. In these primary experiments, it showed that 6HRE-GFAPBaxα system could promote glioma cell apoptosis. It was specific and effective for glioma gene therapy.

关键词： GFAP-Baxα BT325 Fibrillary apoptotic specificity