资源类型

期刊论文 10

年份

2022 3

2016 1

2014 1

2013 1

2009 1

2007 3

关键词

7种气态污染物 1

发酵乳杆菌 1

培养组学 1

基质吸力 1

塑性体应变与剪应变的相互作用原理 1

大肠杆菌 1

废气净化 1

抗炎 1

有效应力原理 1

比较基因组分析 1

生物膜填料塔 1

系统发育分析 1

结直肠癌 1

结肠炎 1

肠道菌群 1

菌株水平 1

菌种鉴定 1

非饱和土 1

展开 ︾

检索范围:

排序: 展示方式:

Selection and characterization of eight freshwater green algae strains for synchronous water purification

Jingjing ZHAN,Qiao ZHANG,Momei QIN,Yu HONG

《环境科学与工程前沿(英文)》 2016年 第10卷 第3期   页码 548-558 doi: 10.1007/s11783-016-0831-4

摘要: The objective of this study is to select and characterize the candidate for synchronous water purification and lipid production from eight freshwater microalgae strains ( sp. HQ, , , , , , , sp. LX1). The strains sp. HQ, . , and . showed superiority in biomass accumulation, while the top biomass producers did not correspond to the top lipid producers. achieved higher lipid content (66.1%), and sp. HQ and ranked down in sequence, with lipid content above 30%. Considering nutrient removal ability (total nitrogen (TN): 52.97%; total phosphorus (TP): 84.81%), the newly isolated microalga sp. HQ was the possible candidate for water purification coupled with lipid production. To further investigate the lipid producing and nutrient removal mechanism of candidate microalga, the ultra structural changes especially the lipid droplets under different water qualities (different TN and TP concentrations) were characterized. The results elucidate the nutrient-deficiency (TN: 3.0 mg·L ; TP: 0.3 mg·L ) condition was in favor of forming lipid bodies in sp. HQ at the sub-cellular level, while the biomass production was inhibited due to the decrease in chloroplast number which could further suppress the nutrient removal effect. Finally, a two-phase cultivation process (a nutrient replete phase to produce biomass followed by a nutrient deplete phase to enhance lipid content) was conducted in a photo-bioreactor for sp. HQ to serve for algae-based synchronous biodiesel production and wastewater purification.

关键词: freshwater microalgae     biomass production     lipid accumulation     nutrient removal    

The molecular epidemiological study of colistin-only-sensitive strains in multi-drug resistant Acinetobacter

YANG Li, HAN Lizhong, SUN Jingyong, NI Yuxing, YU Yunsong

《医学前沿(英文)》 2007年 第1卷 第4期   页码 423-428 doi: 10.1007/s11684-007-0083-6

摘要: This paper reported the epidemiology of the colistin-only-sensitive (COS-AB) in a tertiary teaching hospital in China. We analyzed the clinical data of 136 COS-AB isolates from June 2004 to May 2005 and collected 66 . isolates in which 33 strains were COS-AB, and the rest were non-COS-AB. Random amplified polymorphic DNA (RAPD) analysis (primer ERIC2 and 272) showed that all COS-AB were identical, while pulsed-field gel electrophotesis (PFGE) analysis showed two separate genotypes of these COS-AB which were distinctly different from that of non-COS-AB. The COS-AB from burn wards showed the identical PFGE pattern which was distinguished from the genotype of COS-AB in other departments, mainly surgical systems. The cross-infection was severe and strict methods of disinfection and sterilization should be implemented. Meanwhile, the epidemiology of COS-AB in environment and patients should be closely monitored. The PFGE analysis is a reliable method of . typing.

关键词: identical     different     teaching hospital     genotype     colistin-only-sensitive    

非饱和土的塑性体应变与剪应变的相互作用原理

王靖涛

《中国工程科学》 2007年 第9卷 第11期   页码 11-15

摘要:

将岩土塑性体应变与剪应变的相互作用原理拓展到了非饱和土领域。除了塑性体应变与剪应变的相互作用外,在非饱和土中出现了两类新的相互作用,吸力-塑性体应变和孔隙气压力-塑性体应变。吸力具有二重性质,其对塑性体应变作用包括两个相反的方面。基于吸力性质,阐明了非饱和土的一些独特的性质,诸如有效应力参数的物理涵义,吸力对体积变化和前期固结压力的影响和湿陷机理等。另外,应用拓展的塑性体应变与剪应变相互作用原理,从理论上证明了非饱和土的临界状态线是存在的和唯一的,以及它与应力历史无关。

关键词: 塑性体应变与剪应变的相互作用原理     非饱和土     基质吸力     有效应力原理    

Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones

Zhuoying WU, Qing WANG, Feng GUO, Shenghua ZHANG, Qipei JIANG, Xin YU

《环境科学与工程前沿(英文)》 2014年 第8卷 第2期   页码 205-214 doi: 10.1007/s11783-013-0492-5

摘要: Often as a result of biofilm formation, drinking water distribution systems (DWDS) are regularly faced with the problem of microbial contamination. Quorum sensing (QS) systems play a marked role in the regulation of microbial biofilm formation; thus, inhibition of QS systems may provide a promising approach to biofilm formation control in DWDS. In the present study, 22 bacterial strains were isolated from drinking water-related environments. The following properties of the strains were investigated: bacterial biofilm formation capacity, QS signal molecule N-acyl-L-homoserine lactones (AHLs) production ability, and responses to AHLs and AHL analogs, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and 2(5H)-furanone. Four AHLs were added to developed biofilms at dosages ranging from 0.1?nmol·L to 100 nmol·L . As a result, the biofilm growth of more than 1/4 of the isolates, which included AHL producers and non-producers, were significantly promoted. Further, the biofilm biomasses were closely associated with respective AHLs concentrations. These results provided evidence to support the idea that AHLs play a definitive role in biofilm formation in many of the studied bacteria. Meanwhile, two AHLs analogs demonstrated unexpectedly minimal negative effects on biofilm formation. This suggested that, in order to find an applicable QS inhibition approach for biofilm control in DWDS, the testing and analysis of more analogs is needed.

关键词: drinking water distribution systems (DWDS)     biofilm     quorum sensing (QS)     N-acyl-L-homoserine lactones (AHLs)     (dichloromethyl)-5-hydroxy-2(5H)-furanone (MX)     2(5H)-furanone    

Structural health monitoring with fiber optic sensors

F. ANSARI

《机械工程前沿(英文)》 2009年 第4卷 第2期   页码 103-110 doi: 10.1007/s11465-009-0032-y

摘要: Optical fiber sensors have been successfully implemented in aeronautics, mechanical systems, and medical applications. Civil structures pose further challenges in monitoring mainly due to their large dimensions, diversity and heterogeneity of materials involved, and hostile construction environment. This article provides a summary of basic principles pertaining to practical health monitoring of civil engineering structures with optical fiber sensors. The issues discussed include basic sensor principles, strain transfer mechanism, sensor packaging, sensor placement in construction environment, and reliability and survivability of the sensors.

关键词: Bridges     structural health monitoring     smart skins     cracks     strains     displacements     fiber optic sensors     FBG     structures    

Improvement of the assimilable organic carbon (AOC) analytical method for reclaimed water

Xin ZHAO, Hongying HU, Shuming LIU, Feng JIANG, Xiaolei SHI, Mingtang LI, Xueqiao XU

《环境科学与工程前沿(英文)》 2013年 第7卷 第4期   页码 483-491 doi: 10.1007/s11783-013-0525-0

摘要: Microbial growth is an issue of concern that may cause hygienic and aesthetic problems during the transportation and usage of reclaimed water. Assimilable organic carbon (AOC) is an important parameter which determines the heterotrophic bacterial growth potential of water. P17 and sp. NOX are widely used to measure AOC in drinking water. The AOC values of various reclaimed water samples determined by P17 and NOX were compared with those determined by the new strains isolated from reclaimed water in this study. It showed that the conventional test strains were not suitable for AOC measurement of reclaimed water in certain cases. In addition to P17 and NOX, sp. ZJ2, G3 and sp. G6, were selected as test strains for AOC measurement of reclaimed water. Key aspects of the bioassay including inoculum cell density, incubation temperature, incubation time and the pH of samples were evaluated for the newly selected test strains. Higher inoculum density (10 CFU·mL ) and higher incubation temperature (25°C) could reduce the time required for the tests. The AOC results of various collected samples showed the advantages of the method proposed based on those five strains in evaluating the biologic stability of reclaimed water.

关键词: assimilable organic carbon (AOC)     bioassay     biological stability     reclaimed water     test bacterial strains    

生物法净化几种气态污染物的研究

孙石,王洁,吴献花,李晓梅,许晓毅,邓辅唐,张唯,雷艳梅,曾二丽

《中国工程科学》 2007年 第9卷 第1期   页码 73-77

摘要:

应用生物膜填料塔对工业废气中常见的甲苯 、 苯乙烯 、甲醛 、CS2, SO2 , H2S , NOx 等7种气态污染 物进行净化实验,结果表明,采用专用菌种挂膜制作的生物膜填料塔对该7种气态污染物均有生物净化作用,但其对 SO2, H2S , NOx 等3种无机污染物的生物净化作用(生化去除量可达 90~ 150mg/L·h) 明显优于对甲苯、苯乙烯、甲醛、 二硫化碳等4种有机污染物的净化作用(生化去除量除对甲苯的可达 100 mg/L·h 外,均低于 30 mg/L·h)。 研究确定了净化各污染物的适宜操作条件 , 并对7个专用菌种进行了鉴定 。

关键词: 7种气态污染物     生物膜填料塔     废气净化     菌种鉴定    

结直肠癌黏膜组织来源的大肠杆菌菌株间遗传和功能差异研究 Article

常宇骁, 李享, 丁磊, 杨超, 潘志远, 韩妮, 崔玉军, 智发朝, 杨瑞馥, 高宏,毕玉晶

《工程(英文)》 2022年 第16卷 第9期   页码 210-219 doi: 10.1016/j.eng.2021.03.028

摘要:

结直肠癌是全球第三大癌症。宏基因组学已被广泛用于基于细菌属或种水平的比较来分析肠道微生物群与结直肠癌之间的关系,为结直肠癌发展中的生态失调提供了证据。然而,这种分析并不能为我们提供菌株水平的信息,进而理解一种细菌在结直肠癌发生发展中菌株水平的作用。本文利用培养组学方法分离结直肠癌黏膜样本,并选择了158株大肠杆菌,通过系统发育分析和炎症诱导实验,揭示它们在基因组学和功能上的差异。基因组比较可以将这些菌株分为5个系统群。选择代表性菌株进行THP-1细胞(人白血病单核细胞)Transwell实验以及动物实验,结果显示不同菌株经刺激后,细胞因子水平有显著性差异。进一步的生物信息学分析揭示了不同系统群间单核苷酸多态性、基因和代谢途径的不同特征,这些结果有助于了解这些菌株之间的表型差异。细菌菌株在基因组学和功能上的差异表明,菌株水平上的功能差异可以进一步了解宿主与肠道细菌的相互作用机制。

关键词: 结直肠癌     肠道菌群     培养组学     大肠杆菌     菌株水平    

基于系统发育和比较基因组分析揭示与发酵乳杆菌缓解结肠炎相关的关键基因 Article

赵岩, 张程程, 于雷雷, 田丰伟, 赵建新, 张灏, 陈卫, 翟齐啸

《工程(英文)》 2022年 第17卷 第10期   页码 170-182 doi: 10.1016/j.eng.2020.09.016

摘要:

越来越多的研究表明,发酵乳杆菌可以用于溃疡性结肠炎的预防和治疗。本研究中,我们从中国不同地区的人群粪便样本中分离出了105 株发酵乳杆菌,并对其基因组草图进行了测序。我们分析了这些菌株的泛基因组和系统发育特征,并对4 个模型菌株(发酵乳杆菌3872、CECT5716、IFO3956 和VRI003)也进行了分析。系统发育分析表明,发酵乳杆菌基因组的进化方向与宿主的地理位置、性别、族群和年龄没有明显的关系。我们挑选了3 株来自不同的系统发育支系的发酵乳杆菌(FWXBH115、FGDLZR121和FXJCJ61)和发酵乳杆菌模式菌株CECT5716,通过构建右旋糖酐硫酸钠(DSS)诱导的结肠炎小鼠模型,
探究这几株菌的抗炎和免疫调节活性。发酵乳杆菌FXJCJ61 和CECT5716 可以通过缓解所有结肠炎相关的组织学指标,保护黏膜完整性,增加肠道短链脂肪酸(SCFA),显著减轻结肠炎,而其他两株菌未能提供类似的保护作用。发酵乳杆菌FXJCJ61 和CECT5716 的抗炎机制与核转录因子kappa-B(NF-κB)信号通路激活以及促进白细胞介素10(IL-10)的产生有关。比较基因组分析结果表明,这些有益发酵乳杆菌的抗炎作用可能与一些特定基因有关。

关键词: 发酵乳杆菌     结肠炎     抗炎     系统发育分析     比较基因组分析    

碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播 Article

沈应博, 胡付品, 王永强, 尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R. Walsh, 汪洋

《工程(英文)》 2022年 第15卷 第8期   页码 24-33 doi: 10.1016/j.eng.2021.07.030

摘要:

Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichia coli (CREC), especially New Delhi metallo-β-lactamase (NDM)-producing strains, are widely prevalent in foodproducing animals. At present, the impact of livestock-associated CREC strains on human populations at the national level is unknown. Here, we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence. We also ascertain the blaNDM and blaKPC abundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin. Overall, 631/29799 (2.1%) clinical Escherichia coli (E. coli) isolates were identified as CREC. Multivariable analysis revealed that being male, an age below 1, an age between 13 and 18, provinces with greater chicken production, and provinces with higher pig production were associated with higher odds of CREC infection. In general, 73.8% (n = 45/61) of pig farms and 62.2% (n = 28/45) of chicken farms had a blaNDM abundance of 1×10-5 to 1×10-3 and 1×10-3  to 1×10-2, respectively. Among all the Chinese NDM-positive E. coli (n = 463) available at the National Center for Biotechnology Information (NCBI), the genomic analysis revealed that blaNDM-5 and IncX3 were the predominant carbapenemase gene-plasmid combination, while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels. All the findings suggest frequent CREC transmission between humans and animals, indicating that further discussions on the use of antibiotics in animals and humans are needed, both in China and across the globe.

关键词: Carbapenem resistance     Escherichia coli     blaNDM-5     One Health     Antimicrobial resistance transmission    

标题 作者 时间 类型 操作

Selection and characterization of eight freshwater green algae strains for synchronous water purification

Jingjing ZHAN,Qiao ZHANG,Momei QIN,Yu HONG

期刊论文

The molecular epidemiological study of colistin-only-sensitive strains in multi-drug resistant Acinetobacter

YANG Li, HAN Lizhong, SUN Jingyong, NI Yuxing, YU Yunsong

期刊论文

非饱和土的塑性体应变与剪应变的相互作用原理

王靖涛

期刊论文

Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones

Zhuoying WU, Qing WANG, Feng GUO, Shenghua ZHANG, Qipei JIANG, Xin YU

期刊论文

Structural health monitoring with fiber optic sensors

F. ANSARI

期刊论文

Improvement of the assimilable organic carbon (AOC) analytical method for reclaimed water

Xin ZHAO, Hongying HU, Shuming LIU, Feng JIANG, Xiaolei SHI, Mingtang LI, Xueqiao XU

期刊论文

生物法净化几种气态污染物的研究

孙石,王洁,吴献花,李晓梅,许晓毅,邓辅唐,张唯,雷艳梅,曾二丽

期刊论文

结直肠癌黏膜组织来源的大肠杆菌菌株间遗传和功能差异研究

常宇骁, 李享, 丁磊, 杨超, 潘志远, 韩妮, 崔玉军, 智发朝, 杨瑞馥, 高宏,毕玉晶

期刊论文

基于系统发育和比较基因组分析揭示与发酵乳杆菌缓解结肠炎相关的关键基因

赵岩, 张程程, 于雷雷, 田丰伟, 赵建新, 张灏, 陈卫, 翟齐啸

期刊论文

碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播

沈应博, 胡付品, 王永强, 尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R. Walsh, 汪洋

期刊论文