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Low-temperature caproate production, microbial diversity, and metabolic pathway in xylose anaerobic fermentation

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1637-9

摘要：

● Converting xylose to caproate under a low temperature of 20 °C by MCF was verified.

关键词： Xylose fermentation     Caproate     Low temperature     Bifidobacterium     FAB pathway     RBO pathway    

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Construction, characterization and application of a genome-wide promoter library in

Ting Yuan,Yakun Guo,Junkai Dong,Tianyi Li,Tong Zhou,Kaiwen Sun,Mei Zhang,Qingyu Wu,Zhen Xie,Yizhi Cai,Limin Cao,Junbiao Dai

《化学科学与工程前沿（英文）》 2017年 第11卷 第1期   页码 107-116 doi: 10.1007/s11705-017-1621-7

摘要： Promoters are critical elements to control gene expression but could behave differently under various growth conditions. Here we report the construction of a genome-wide promoter library, in which each native promoter in was cloned upstream of a yellow fluorescent protein (YFP) reporter gene. Nine libraries were arbitrarily defined and assembled in bacteria. The resulting pools of promoters could be prepared and transformed into a yeast strain either as centromeric plasmids or integrated into a genomic locus upon enzymatic treatment. Using fluorescence activated cell sorting, we classified the yeast strains based on YFP fluorescence intensity and arbitrarily divided the entire library into 12 bins, representing weak to strong promoters. Several strong promoters were identified from the most active bins and their activities were assayed under different growth conditions. Finally, these promoters were applied to drive the expression of genes in xylose utilization to improve fermentation efficiency. Together, this library could provide a quick solution to identify and utilize desired promoters under user-defined growth conditions.

关键词： synthetic biology     yeast     promoter activity     metabolic engineering     xylose utilization    

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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

《化学科学与工程前沿（英文）》 2018年 第12卷 第4期   页码 772-779 doi: 10.1007/s11705-018-1731-x

摘要： We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet- T7- T7- was constructed and transformed to BL21(DE3) to obtain the whole cells of BL21- capable of bioconversion D-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L yeast extract, 5 g·L sucrose, 3g·L KH PO , 5 g·L NaCl, 3g·L NH Cl, 0.25 g·L MgSO ?7H O and 1 mL·L the mixture of trace elements. With the optimized whole cells of BL21- , 60 g·L of xylose was converted to 28g·L BT with a molar yield of 66 %, which is higher than those reported in the biotechnological system.

关键词： D-1     2     4-butanetriol     whole-cell bioconversion     carbon source     nitrogen sources     metal ions     culture conditions    

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Advanced purification and comprehensive utilization of yellow phosphorous off gas

Ping NING,Xiangyu WANG

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 181-189 doi: 10.1007/s11783-014-0698-1

摘要： Yellow phosphorous is an important raw material in the chemical industry. However, during the production of yellow phosphorous, high concentrations of carbon monoxide and other impurities are released. Without appropriate purification and removal, this off gas has potential to cause severe pollution problems once released. Purified yellow phosphorous off gas can be beneficially reused as a raw material in chemical production for synthesis of high value-added chemical reagents. In this paper, the significance of purification and reutilization of yellow phosphorous off gas are explored. The principles, processes, and main characteristics of the technologies for purification and reuse of yellow phosphorus off gas (including technical measurements of impurity reduction, relevant engineering cases, and public acceptance of the technologies) are summarized. In view of the existing problems and scientific development requirements, this paper proposes several recommendations for green production based on the concept of recycle economics. We conclude that advanced purification and comprehensive reutilization can be an effective solution for heavy pollution resulting from yellow phosphorous off gassing.

关键词： yellow phosphorous off gas     purification     comprehensive utilization    

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Methodologies for chemical utilization of CO

Liangnian HE, Ya Du, Chengxia MIAO, Jinquan WANG, Xiaoyong DOU, Ying WU

《化学科学与工程前沿（英文）》 2009年 第3卷 第2期   页码 224-228 doi: 10.1007/s11705-009-0067-y

摘要： The reactions of CO with oxirane to produce cyclic carbonate, and with aziridine to afford oxazolidine have been of interest as a useful method for its fixation by a chemical process. Highly efficient processesemploying recyclable CO -phlilic homogeneous catalyst were devised for environmentally benign synthesis of cyclic carbonates and oxazolidinones under supercritical CO without any organic solvent. These processes represent pathways for greener chemical fixations of CO to afford industrial useful materials such as organic carbonates and oxazolidinones with great potential applications.

关键词： carbon dioxide     chemical utilization     molecular catalyst     carbonate     oxazolidinone    

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CROP PROTECTION OPENS UP NEW ERA OF CONSERVATION AND UTILIZATION OF GREEN OPTIONS

《农业科学与工程前沿（英文）》 2022年 第9卷 第1期   页码 1-3 doi: 10.15302/J-FASE-2021431

摘要：

China is the largest agricultural producer in the world. Reducing yield losses caused by pests is an important issue and major challenge for China, especially when confronting global climate change, biological invasions and declining agricultural biodiversity of recent decades. Wang et al. (this issue) summarized the impacts of changing climate on two staple crops in China, wheat and rice (https://doi.org/FASE-2021432). They reviewed the impacts of climate change on crops, crop pests and crop diseases. Basically, increased temperature would reduce crop yields and increase pest damage. Biological invasions have become a serious threat to the agriculture worldwide. Developing approaches for monitoring and controlling invasive pests, such as fall armyworm, has been recognized as high priority for China. The excessive use of highly toxic pesticides has caused considerable damage to the environment and biodiversity, which is also one of the many contributors to the increasing outbreaks of pests and diseases. In addition, understanding interaction between crop pests and plant diseases is another critical challenge for agriculture. About 80% plant viruses can be transmitted by crop pest vectors such as aphids, whiteflies and other hemipteran pests. Therefore, to solve the problem of crop pests and diseases, suitable green crop protection methods have been identified as a key components of sustainable development. This special issue of Frontiers of Agricultural Science and Engineering outlines potential conservation and utilization of green options for crop protection.

To prevent outbreaks of crop pests, an advanced monitoring and early warning system could be the first step in crop protection. The use of science and technology in monitoring and early warning of major crop pests provides better pest management and acts as a fundamental part of an integrated plant protection strategy to achieve the goal of sustainable development of agriculture. Wu et al. (this issue) summarized the fundamental information on pest monitoring and early warning in China by documenting the history of research and application, Chinese laws and regulations related to plant protection, and the National Monitoring and Early Warning System, with the purpose of presenting the Chinese model as an example of how to promote regional management of crop pests, especially cross border pests such as fall armyworm and locust, by international cooperation across pest-impacted countries (https://doi.org/10.15302/J-FASE-2021411). They provide an overview of long-term Chinese efforts in the fight against major pests and to present the country’s experience in crop pest monitoring and early warning technology to the world.

Field management is a traditional conservation agriculture technique on plant protection such as no-till cropping, crop rotation, intercropping and cover cropping. Shi et al. (this issue) found that soil solarization is an environmental-friendly promising strategy that achieved complete mortality to the larvae of Bradysia cellarum (https://doi.org/10.15302/J-FASE-2021402). Crop rotation, intercropping and cover cropping of different crops or plants could help to reduce the outbreak of crop pests and diseases. Wang et al. (this issue) summarized the interactions and trade-offs between plants and entomophagous arthropods, and discussed a landscape management method by sowing flowering plants in agricultural fields (https://doi.org/10.15302/J-FASE-2021427). However, such methods are limited when crop pests have already become well established in crop fields.

Pesticides are widely used in the crop protection. The ideal pesticide should have high effectiveness in the control crop pests but low toxicity to non-target organisms. In this case, biopesticides including biochemical pesticides, microbial pesticides and plant-incorporated protectants are considered to be potential ideal pesticides for crop protection. Biochemical pesticides are the naturally occurring substances, they are derived from animals, plants and microorganisms. Microbial pesticides mainly rely on bacteria, fungi, virus or protozoans as the active agent. They are environment-friendly biopesticides with high effectiveness and species-specificity, and have been commercialized worldwide, for example, Bacillus thuringiensis. Plant-incorporated protectants are products that are genetically incorporated into plant tissues including chemicals, proteins or dsRNA for pest control or anti-microbial activity. Qu et al. (this issue) systematically introduced bioinsecticides for pest control and discussed current constraints that prevent bioinsecticides from being widely used and proposed the future research directions in this issue (https://doi.org/10.15302/J-FASE-2021404). Xu et al. (this issue) optimized synthesis process of an anti-plant-virus candidate drug NK0238, and evaluated the antivirus activity and environmental safety of this product (https://doi.org/10.15302/J-FASE-2021390). Zhu et al. (this issue) investigated a new family of natural cyclic lipodepsipeptides with potent activity against Gram-positive bacteria. By genetic manipulation of accessory genes in the WAP biosynthetic gene cluster, new WAP-8294As were produced in Lysobacter, which confirmed the possibility to expand the spectrum of the biocontrol compounds (https://doi.org/10.15302/J-FASE-2021410). In addition, structural biology studies on action targets provides important insights on molecular functions. Using a structure-based drug design strategy, Zheng et al. (this issue) prepared a battery of novel triketone-quinoxaline compounds, which could be potentially applied to design new herbicides targeting p-hydroxyphenylpyruvate dioxygenase (https://doi.org/10.15302/J-FASE-2021401).

With the development and application of new biotechnology in agriculture, opportunities for crop protection are great becoming more abundant, opening new approaches, such as genetic engineering, to improve the plant resistance against crop pests and diseases. It is now feasible to control pests by directly targeting genes that are essential for the development and survival of insects and pathogenic microorganisms, for example, the sterile insect technique based on gene-drive and RNA interference using dsRNA. In addition, it has been confirmed that some gene families are effective targets that indirectly contribute to crop pests and diseases protection by targeting the plant biochemical synthesis or virus transmitting pathways. In this case, identifying new genes associated with the interaction between crops, insects, microorganisms should be a key focus, both now and in the future. Huang et al. (this issue) summarized the recent research on the interaction between plant viruses and insect vectors, and discussed the potential control strategies to prevent the transmission of insect-vectored plant viruses using RNAi technology, gene editing technology and gene-driven technology (https://doi.org/10.15302/J-FASE-2021389).

We anticipate that the ideas and approaches highlighted in this issue will help broaden reader’s perspectives on every increasing prospect for a new era of sustainable and environment-friendly agricultural pest management.

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Saline-alkali soil reclamation and utilization in China: progress and prospects REVIEW

《农业科学与工程前沿（英文）》 2024年 第11卷 第2期   页码 216-228 doi: 10.15302/J-FASE-2024551

摘要：

Soil salinity is a global threat to the productivity of arable land. With the impact of population growth and development of social economy in China, the area of arable land has been shrinking in recent decades and is approaching a critical threshold of 120 Mha, the minimum area for maintaining the national food security. Saline-alkaline land, as important backup reserve, has been receiving increased attention as an opportunity to expand land resources. This review first summarizes the general principles and technologies of saline soil reclamation to support plant growth, including leaching salts or blocking the rise of salts, and soil fertility enhancement to improve the buffering capacity. Then the progress in this area in China is described including the customization of technologies and practices used in different saline-alkali regions. Following the soil management strategies, the concept of selecting crops for saline soil is proposed. This encompasses halophyte planting, salt-tolerant crop breeding and the application of saline-adapted functional microorganisms to improve the adaptation of crops. Finally, the current problems and challenges are evaluate, and future research directions and prospects proposed for managing this major soil constraint.

关键词： Food security     land reserve     reclamation     saline-alkali soil     utilization    

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Recent development of vibration utilization engineering

WEN Bangchun

《机械工程前沿（英文）》 2008年 第3卷 第1期   页码 1-9 doi: 10.1007/s11465-008-0017-2

摘要： The utilization of vibration and wave, which was developed during the latter half of the 20th century, is one of the most valueable technology applications and has been rapidly developing recently . Because the technique is closely associated with industry and agriculture, it can create huge social and economical benefits and provide excellent services for society. Thus, due to its necessity in industry and daily life, extensive research has been devoted to vibration utillization engineering. In this paper, vibration utilization is classified into linear or non-linear vibrations, waves, and electric-magnetic oscillations. Their phenomena, patterns, and applications in nature and society are introduced. Some research results about vibration utilization engineering are described.

关键词： utilization engineering     non-linear     vibration     vibration utillization     utillization engineering    

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China’s policy framework for carbon capture, utilization and storage: Review, analysis, and outlook

《能源前沿（英文）》 2023年 第17卷 第3期   页码 400-411 doi: 10.1007/s11708-023-0862-z

摘要： Carbon capture, utilization, and storage (CCUS) is estimated to contribute substantial CO2 emission reduction to carbon neutrality in China. There is yet a large gap between such enormous demand and the current capacity, and thus a sound enabling environment with sufficient policy support is imperative for CCUS development. This study reviewed 59 CCUS-related policy documents issued by the Chinese government as of July 2022, and found that a supporting policy framework for CCUS is taking embryonic form in China. More than ten departments of the central government have involved CCUS in their policies, of which the State Council, the National Development and Reform Commission (NDRC), the Ministry of Science and Technology (MOST), and the Ministry of Ecological Environment (MEE) have given the greatest attention with different focuses. Specific policy terms are further analyzed following the method of content analysis and categorized into supply-, environment- and demand-type policies. The results indicate that supply-type policies are unbalanced in policy objectives, as policy terms on technology research and demonstration greatly outnumber those on other objectives, and the attention to weak links and industrial sectors is far from sufficient. Environment-type policies, especially legislations, standards, and incentives, are inadequate in pertinence and operability. Demand-type policies are absent in the current policy system but is essential to drive the demand for the CCUS technology in domestic and foreign markets. To meet the reduction demand of China’s carbon neutral goal, policies need to be tailored according to needs of each specific technology and implemented in an orderly manner with well-balanced use on multiple objectives.

关键词： carbon capture     utilization     and storage (CCUS)     policy     content analysis     China    

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Analysis and prediction of the influence of energy utilization on air quality in Beijing

LI Lin, HAO Jiming, HU Jingnan

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 339-344 doi: 10.1007/s11783-007-0058-5

摘要： This work evaluates the influence of energy consumption on the future air quality in Beijing, using 2000 as the base year and 2008 as the target year. It establishes the emission inventory of primary PM, SO and NO related to energy utilization in eight areas of Beijing. The air quality model was adopted to simulate the temporal and spatial distribution of each pollutant concentration in the eight urban areas. Their emission, concentration distribution, and sectoral share responsibility rate were analyzed, and air quality in 2008 was predicted. The industrial sector contributed above 40% of primary PM and SO resulting from energy consumption, while vehicles accounted for about 65% of NO. According to the current policy and development trend, air quality in the eight urban areas could become better in 2008 when the average concentrations of primary PM, SO and NO related to energy utilization at each monitored site are predicted to be about 25, 50 and 51 μg/m, respectively.
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Special issue on electrochemical conversion and utilization of hydrogen energy

《能源前沿（英文）》 2024年 第18卷 第3期   页码 263-264 doi: 10.1007/s11708-024-0944-6

摘要： Special issue on electrochemical conversion and utilization of hydrogen energy

关键词： hydrogen energy    

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Industrial waste utilization method: producing poly-ferric sulfate (PFS) from sodium-jarosite residue

Zhongguo LI,Wenyi YUAN

《环境科学与工程前沿（英文）》 2015年 第9卷 第4期   页码 731-737 doi: 10.1007/s11783-014-0687-4

摘要： Sodium-jarosite is a type of industrial waste that results from hydrometallurgy and inorganic chemical production. The iron content of jarosite residue may be utilized to produce theoretically the ferrous materials. The difficulty in production of high quality poly-ferric sulfate (PFS) is how to remove impurities contained in jarosite residue. This paper proposes a novel method for disposing sodium-jarosite which can be used to synthesize PFS, a very important reagent for treating waste water. The method consists of a two-step leaching experimental procedures. The first step, pre-leaching process, is to remove impurity metals by strictly controlling the leaching conditions. The acid concentration of acidic water was adjusted according to the content of impurity metals in sodium-jarosite and the leaching temperature was controlled at 25°C. The second step is to decompose sodium-jarosite to provide enough ferric ions for synthesizing PFS, the concentrated sulfuric acid consumption was 0.8 mL·g sodium-jarosite and the leaching temperature was above 60°C. In the experiment, decomposing iron from sulfate sodium-jarosite can take the place of ferric martials for synthesizing PFS. Results show that the PFS synthesized from sodium-jarosite had a high poly-iron complex Fe (SO ) (OH) ·20H O. Further, the PFS product’s specifications satisfied the national standard of China.

关键词： sodium-jarosite residue     utilization     poly-ferric sulfate (PFS)    

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Synergistic utilization of coal and other energy – Key to low carbon economy

Weidou NI, Zhen CHEN

《能源前沿（英文）》 2011年 第5卷 第1期   页码 1-19 doi: 10.1007/s11708-010-0136-4

摘要： In China, coal is a dominant component of energy mix, and it is expected to remain as such over the next 30 to 40 years. Coal is expected to be used even more in power generation. The direct combustion of coal already has been causing severe pollution and ecological degradation, and it is quite difficult to address the need to reduce greenhouse gas (GHG) given the direct combustion of coal. Therefore, the polygeneration system based on coal gasification, which is one of the major examples of synergistic utilization of coal, is proposed. It is a comprehensive solution to meet the energy challenges China is facing. Furthermore, the synergy of fossil fuels (especially coal) with renewable energy, the synergy of different kinds of energy for energy storage, the synergy of centralized and distributed supply of different kinds of energy, and the synergy of different kinds of energy in smart energy grid (power, gas, heat, and water) are the keys to making China a low-carbon economy. Carbon dioxide (CO ) mitigation in China should begin from the coal-chemical industry given their accumulated relevant experiences. The mitigation process should gradually be transformed into the “IGCC+ polygeneration+ CCUS”. The objectives of this paper are to describe the synergistic utilization of coal, and to analyze the synergy of coal with other energy resources, and to propose the scientific and technological problems to achieve these synergies.

关键词： synergy     clean and efficient utilization of coal     coal-based polygenration     CO2 mitigation     energy storage    

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Research on recycled concrete and its utilization in building structures in China

Jianzhuang XIAO, Tao DING

《结构与土木工程前沿（英文）》 2013年 第7卷 第3期   页码 215-226 doi: 10.1007/s11709-013-0212-z

摘要： Large quantities of construction and demolition (C&D) building waste are being generated as a result of rapid urbanization and natural disasters in China. An increasing awareness of environmental protection is escalating C&D waste disposal concerns. This paper presents a brief introduction to current shaking table test research in China on structures built with recycled aggregate concrete (RAC). Test structures include a cast-in situ frame model, a precast frame model and a block masonry building. The test results prove that it is feasible to use RAC as a structural material in seismic areas, with recommended modifications and proper design, especially in low-rise structures. This paper also presents several successful applications of RAC in civil and structural engineering projects in China, which will serve to promote RAC as a global ecological structural material.

关键词： recycled aggregate concrete (RAC)     structural material     shaking table tests     building structure    

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Clean and highly-efficient utilization of coal

Guangxi YUE, Junfu LYU, Shuiqing LI

《能源前沿（英文）》 2021年 第15卷 第1期   页码 1-3 doi: 10.1007/s11708-021-0724-5

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标题 作者 时间 类型 操作

Low-temperature caproate production, microbial diversity, and metabolic pathway in xylose anaerobic fermentation

期刊论文

Construction, characterization and application of a genome-wide promoter library in

Ting Yuan,Yakun Guo,Junkai Dong,Tianyi Li,Tong Zhou,Kaiwen Sun,Mei Zhang,Qingyu Wu,Zhen Xie,Yizhi Cai,Limin Cao,Junbiao Dai

期刊论文

Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

期刊论文

Advanced purification and comprehensive utilization of yellow phosphorous off gas

Ping NING,Xiangyu WANG

期刊论文

Methodologies for chemical utilization of CO

Liangnian HE, Ya Du, Chengxia MIAO, Jinquan WANG, Xiaoyong DOU, Ying WU

期刊论文

CROP PROTECTION OPENS UP NEW ERA OF CONSERVATION AND UTILIZATION OF GREEN OPTIONS

期刊论文

Saline-alkali soil reclamation and utilization in China: progress and prospects

期刊论文

Recent development of vibration utilization engineering

WEN Bangchun

期刊论文

China’s policy framework for carbon capture, utilization and storage: Review, analysis, and outlook

期刊论文

Analysis and prediction of the influence of energy utilization on air quality in Beijing

LI Lin, HAO Jiming, HU Jingnan

期刊论文

Special issue on electrochemical conversion and utilization of hydrogen energy

期刊论文

Industrial waste utilization method: producing poly-ferric sulfate (PFS) from sodium-jarosite residue

Zhongguo LI,Wenyi YUAN

期刊论文

Synergistic utilization of coal and other energy – Key to low carbon economy

Weidou NI, Zhen CHEN

期刊论文

Research on recycled concrete and its utilization in building structures in China

Jianzhuang XIAO, Tao DING

期刊论文

Clean and highly-efficient utilization of coal

Guangxi YUE, Junfu LYU, Shuiqing LI

期刊论文