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基于同一健康策略探究老挝人、动物和环境中多黏菌素耐药性的传播特征 Article
周宇晴, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh
《工程（英文）》 2022年 第15卷 第8期 页码 45-56 doi: 10.1016/j.eng.2022.01.013
This study was designed to investigate the molecular epidemiology of mobile colistin resistance (mcr) using a "One-Health" approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination of mcr. We collected 673 samples from humans (rectal normal flora), poultry, and the environment (water, flies, birds, etc.) in Vientiane, Lao People's Democratic Republic (Laos), from May to September 2018. A total of 238 Escherichia coli (E. coli) isolated from nonduplicative samples, consisting of 98 MCR-positive E. coli (MCRPEC) ("mcr" denotes the gene encoding mobile colistin resistance, and "MCR" denotes the subsequent protein encoded by mcr) and 140 MCRnegative E. coli (MCRNEC), were characterized by phenotype and Illumina sequencing. A subset of MCRPEC was selected for MinION sequencing, conjugation assay, plasmid stability, and growth kinetics in vitro. The prevalence of MCRPEC was found to be 14.6% (98/673), with the highest prevalence in human rectal swabs (45.9% (45/98), p < 0.0001, odds ratio (OR): 0.125, 95% confidence interval (CI): 0.077–0.202). The percentages of MCRPEC from other samples were 14.3% (2/14) in dog feces, 12.0% (24/200) in flies, 11.0% (11/100) in chicken meat, 8.9% (8/90) in chicken cloacal, 8.0% (4/50) in chicken caeca, and 7.5% (4/53) in wastewater. MCRPEC was significantly more resistant to co-amoxiclav, sulfamethoxazoletrimethoprim, levofloxacin, ciprofloxacin, and gentamicin than MCRNEC (p < 0.05). Genomic analysis revealed the distribution of MCRPEC among diverse clonal types. The putative plasmid Inc types associated with mcr-1 were IncX4, IncHI2, IncP1, IncI2, and IncFIA, and those associated with mcr-3 were IncFII, IncFIA, IncFIB, IncP1, and IncR. Recovery of highly similar plasmids from both flies and other sampling sectors implied the role of flies in the dissemination of mcr-1. mcr-positive plasmids were shown to be conjugative, and a significantly high transfer rate into a hypervirulent clone ST1193 was observed. Plasmids containing mcr irrespective of Inc type were highly stable and invariably did not exert a fitness effect upon introduction into a new host. These findings signify the urgent need for a standard infection control program to radically decontaminate the source of resistance.
畅华仪,张俊飚,张蕙杰,韦显恒 ,傅廷栋
《中国工程科学》 2019年 第21卷 第4期 页码 100-104 doi: 10.15302/J-SSCAE-2019.04.012
随着“一带一路”倡议的不断推进，中国与老挝在农业科技合作领域不断深入。本文基于对“中国（广西）-老挝农作物优良品种试验站”项目的调查，回顾了目前中-老农业科技合作的实施进展与效果，并对相应合作经验与问题进行了总结。
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基于同一健康策略探究老挝人、动物和环境中多黏菌素耐药性的传播特征
周宇晴, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh
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