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Clinical factors associated with composition of lung microbiota and important taxa predicting clinicalprognosis in patients with severe community-acquired pneumonia

《医学前沿（英文）》 2022年 第16卷 第3期   页码 389-402 doi: 10.1007/s11684-021-0856-3

摘要： Few studies have described the key features and prognostic roles of lung microbiota in patients with severe community-acquired pneumonia (SCAP). We prospectively enrolled consecutive SCAP patients admitted to ICU. Bronchoscopy was performed at bedside within 48 h of ICU admission, and 16S rRNA gene sequencing was applied to the collected bronchoalveolar lavage fluid. The primary outcome was clinical improvements defined as a decrease of 2 categories and above on a 7-category ordinal scale within 14 days following bronchoscopy. Sixty-seven patients were included. Multivariable permutational multivariate analysis of variance found that positive bacteria lab test results had the strongest independent association with lung microbiota (R2=0.033; P=0.018), followed by acute kidney injury (AKI; R2=0.032; P=0.011) and plasma MIP-1β level (R2=0.027; P=0.044). Random forest identified that the families Prevotellaceae, Moraxellaceae, and Staphylococcaceae were the biomarkers related to the positive bacteria lab test results. Multivariable Cox regression showed that the increase in α-diversity and the abundance of the families Prevotellaceae and Actinomycetaceae were associated with clinical improvements. The positive bacteria lab test results, AKI, and plasma MIP-1β level were associated with patients’ lung microbiota composition on ICU admission. The families Prevotellaceae and Actinomycetaceae on admission predicted clinical improvements.

关键词： severe community-acquired pneumonia     lung microbiota     clinical improvements     7-category ordinal scale     Prevotellaceae    

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Effects of heavy rainfall on the composition of airborne bacterial communities

Gwang Il Jang, Chung Yeon Hwang, Byung Cheol Cho

《环境科学与工程前沿（英文）》 2018年 第12卷 第2期 doi: 10.1007/s11783-018-1008-0

摘要： Wet deposition scavenges particles and particle-associated bacteria from the air column, but the impact of raindrops on various surfaces on Earth causes emission of surface-associated bacteria into the air column. Thus, after rainfall, these two mechanisms are expected to cause changes in airborne bacterial community composition (BCC). In this study, aerosol samples were collected at a suburban site in Seoul, Korea before and after three heavy rainfall events in April, May, and July 2011. BCC was investigated by pyrosequencing the 16S rRNA gene in aerosol samples. Interestingly, the relative abundance of non-spore forming operational taxonomic units (OTUs) was always higher in post-rain aerosol samples. In particular, the absolute and relative abundances of airborne always increased after rainfall, whereas those of airborne , including and , consistently decreased. Marine bacterial sequences, which were temporally important in aerosol samples, also decreased after rainfall events. Further, increases in pathogen-like sequences were often observed in post-rain air samples. Rainfall events seemed to affect airborne BCCs by the combined action of the two mechanisms, with potentially adverse effects on human and plant health.

关键词： Aerosol     Bacteria     Community composition     Pyrosequencing     Rain    

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Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 539-547 doi: 10.1007/s11783-016-0830-5

摘要： This study employed 454-pyrosequencing to investigate microbial and pathogenic communities in two wastewater reclamation and distribution systems. A total of 11972 effective 16S rRNA sequences were acquired from these two reclamation systems, and then designated to relevant taxonomic ranks by using RDP classifier. The Chao index and Shannon diversity index showed that the diversities of microbial communities decreased along wastewater reclamation processes. was the most dominant phylum in reclaimed water after disinfection, which accounted for 83% and 88% in two systems, respectively. Human opportunistic pathogens, including , , and , were selected and enriched by disinfection processes. The total chlorine and nutrients (TOC, NH -N and NO -N) significantly affected the microbial and pathogenic communities during reclaimed water storage and distribution processes. Our results indicated that the disinfectant-resistant pathogens should be controlled in reclaimed water, since the increases in relative abundances of pathogenic bacteria after disinfection implicate the potential public health associated with reclaimed water.

关键词： wastewater reclamation systems     microbial community     pathogenic community     454-pyrosequencing    

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The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Yadong YANG, Xiaomin FENG, Yuegao HU, Zhaohai ZENG

《农业科学与工程前沿（英文）》 2019年 第6卷 第2期   页码 162-171 doi: 10.15302/J-FASE-2018212

摘要：

In this study, the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil in three cropping systems and at two oat growth stages were investigated using real-time PCR and Illumina MiSeq sequencing. The gene abundance in oat-soybean intercropping (OSO) and oat-mungbean intercropping (OMO) was significantly greater than that in sole oat (O), but the gene abundance significantly decreased at the later stage in all the treatments. Alpha diversity indices in OSO and OMO were higher at the heading stage, but lower at the maturity stage than that in O. and were the dominant genera identified in all samples, with an average proportion of 35.8% and 12.4%, respectively. The proportion of dominant genera showed significant differences and varied with cropping system and growth stage. Principal component analysis showed that growth stage had a stronger effect than intercropping on the diazotrophic community structure. However, Mantel test and redundancy analysis showed there was no environmental factor significantly correlated to the diazotrophic community structure. Our results demonstrate that intercropping had a weaker effect than growth stage on the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil.

关键词： community composition     Illumina MiSeq sequencing     nifH gene     oat-legume intercropping     rhizosphere soil    

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Impacts of backwashing on micropollutant removal and associated microbial assembly processes in sand filters

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1634-z

摘要：

● Backwashing in sand filters with 2-h and 4-h EBCTs was simulated.

关键词： Sand filter     Backwashing     Recovery     Micropollutants     Community composition    

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Distribution of phytoplankton community and its influence factors in an urban river network, East China

Ling Sun, Hui Wang, Yuanqing Kan, Shiliang Wang

《环境科学与工程前沿（英文）》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1062-7

摘要：

Effects of urban river conditions on phytoplankton community were investigated.

Nitrate utilization and hydrodynamic condition affected phytoplankton distribution.

A winter bloom was induced by abundant ammonia, total phosphorus and organic matters.

The diatoms and euglenoids dominated in an urban beheaded river ecosystem.

关键词： Phytoplankton composition     Spatiotemporal distribution     Functional groups     Nitrate nitrogen     Winter bloom    

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Microbial responses to the use of NaClO in sediment treatment

《环境科学与工程前沿（英文）》 2022年 第16卷 第2期 doi: 10.1007/s11783-021-1451-1

摘要：

• Chlorine addition enhanced the release of TOC, TN from the sediment.

关键词： Sediment chlorination     Substance mobility     Microbial response     Community composition     Function    

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Process stability and microbial community composition in pig manure and food waste anaerobic co-digesters

Conor Dennehy, Peadar G. Lawlor, Gillian E. Gardiner, Yan Jiang, Paul Cormican, Matthew S. McCabe, Xinmin Zhan

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0923-9

摘要： This study assessed the effects of reducing hydraulic retention times (HRTs) from 21 days to 10.5 days when anaerobically co-digesting pig manure and food waste. Continuously stirred tank reactors of 3.75 L working volume were operated in triplicate at 42°C. Digester HRT was progressively decreased from 21 to 15 days to 10.5 days, with an associated increase in organic loading rate (OLR) from 3.1 kg volatile solids (VS)·m ·day to 5.1 kg VS·m ·day to 7.25 kg VS·m ·day . Reducing HRT from 21 days to 15 days caused a decrease in specific methane yields and VS removal rates. Operation at a HRT of 10.5 days initially resulted in the accumulation of isobutyric acid in each reactor. High throughput 16S rRNA gene sequencing revealed that this increase coincided with a shift in acidogenic bacterial populations, which most likely resulted in the increased isobutyric acid concentrations. This may in turn have caused the increase in relative abundance of bacteria, which syntrophically degrade non-acetate volatile fatty acids (VFAs) into H and CO . This, along with the increase in abundance of other syntrophic VFA oxidizers, such as suggests that VFA oxidation plays a role in digester operation at low HRTs. Reducing the HRT to below 21 days compromised the ability of the anaerobic digestion system to reduce enteric indicator organism counts below regulatory limits.

关键词： Biogas     Sequencing     Clocamonaceae     Spiorchatetes     Isobutyrate     Biosafety    

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湿地芦苇根系微生物群落组成与环境应用 Review

王东麟, 柏耀辉, 曲久辉

《工程（英文）》 2022年 第9卷 第2期   页码 42-50 doi: 10.1016/j.eng.2021.05.016

摘要：

芦苇是自然界广泛存在的湿地植物。在人工湿地中，约15.5%的种植植物是芦苇，其在废水水质净化中起到重要的作用。尽管如此，对芦苇根系微生物群落的基本组成及每一类微生物在污染物去除中的作用仍不清晰。本文总结了已有对芦苇根系微生物群落（包括细菌、古菌及真菌）在生态及生化方面的研究。基于下一代测序，对不同环境条件下的微生物群落组成进行了分析。此外，利用培养方法进一步研究了微生物群落的功能特征，如铁的固定、有机物的降解、营养元素的转化等。芦苇根系微生物群落独特的结构和功能受芦苇种类及盐度等环境因素的高度影响。基于现有对芦苇根系微生物群落的研究，我们建议在人工湿地中应用和强化合成微生物群落和铁锰氧化物基质，以提高湿地的水净化性能。

关键词： 芦苇     根系微生物     微生物群落组成     污染修复     人工湿地    

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“Composition” and regularisation of architectural production in contemporary China

Hua LI

《结构与土木工程前沿（英文）》 2010年 第4卷 第4期   页码 465-473 doi: 10.1007/s11709-010-0097-z

摘要： The purpose of this paper is to explore how the “Beaux-Arts” “composition” sustained the constitution of design principles for architectural production in China in the last two decades of the twentieth century. “Composition”, a key technique of the “Beaux-Arts” system, is not a simple act of “putting elements together” into a unified whole, but also relates to an array of different “constructions” of architectural knowledge which regulate and sustain the act of design. Through analysing the structure of three “textbooks” published in the early 1980s, this paper examines three aspects of the “constructions”: the setting up of the principles for governing architectural forms, regularisation of design methods and approaches for architectural practice, and the construction of a “tradition” for the expression of “Chineseness”.

关键词： composition     “Beaux-Arts”     regulate     architectural production    

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Calorie restriction and its impact on gut microbial composition and global metabolism

Xiaojiao Zheng, Shouli Wang, Wei Jia

《医学前沿（英文）》 2018年 第12卷 第6期   页码 634-644 doi: 10.1007/s11684-018-0670-8

摘要：

Calorie restriction (CR) is a dietary regimen that reduces calorie intake without incurring malnutrition or a reduction in essential nutrients. It has long been recognized as a natural strategy for promoting health, extending longevity, and prevents the development of metabolic and age-related diseases. In the present review, we focus on the general effect of CR on gut microbiota composition and global metabolism. We also propose mechanisms for its beneficial effect. Results showed that probiotic and butyrate-producing microbes increased their relative abundance, whereas proinflammatory strains exhibited suppressed relative abundance following CR. Analyses of the gut microbial and host metabolisms revealed that most host microbial co-metabolites were changed due to CR. Examples of dramatic CR-induced changes in host metabolism included a decrease in the rate of lipid biosynthesis and an increase in the rates of fatty acid catabolism, β-oxidation, glycogenolysis, and gluconeogenesis. The observed phenotypes and the further verification of the direct link between gut microbiota and metabolome may benefit patients that are at risk for developing metabolic disease. Thus, improved gut microbiota composition and metabolome are potential biomarkers for determining the effectiveness of dietary interventions for age-related and metabolic diseases.

关键词： caloric restriction     gut microbiota     metabolome    

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Fungal diversity and its mechanism of community shaping in the milieu of sanitary landfill

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1370-6

摘要：

• Ascomycota was the predominant phylum in sanitary landfill fungal communities.

关键词： Sanitary landfill     Fungal community     Diversity     Saprotroph     Physical habitat     Environmental factor    

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Effect of wastewater composition on the calcium carbonate precipitation in upflow anaerobic sludge blanket

Shucheng YANG, Yanling HE, Charles CHOU, Pengxiang ZHANG, Dongqi WANG, Yonghong LIU,

《环境科学与工程前沿（英文）》 2010年 第4卷 第2期   页码 142-149 doi: 10.1007/s11783-010-0026-3

摘要： Calcium carbonate often precipitates in anaerobic reactors treating wastewater with high calcium content. The aim of this paper is to study the effect of wastewater composition on calcium carbonate precipitation in upflow anaerobic sludge blanket (UASB) reactors. Two laboratory-scale UASB reactors were operated with calcium-containing influents using acetate and carbohydrate as substrate, respectively. There was an obvious accumulation of inorganic precipitate observed in the biogranules. Observations via scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) showed that the acclimated biogranules in the two reactors differed in microstructure. Calcium carbonate was found to have precipitated on the surface of acetate-degrading biogranules, but precipitated at the core of the carbohydrate-degrading biogranules. The results indicated that substrates had significant influence on the location of calcium carbonate precipitation in anaerobic granular sludge, which was expected due to the different methanogens distribution and pH gradient within the granular sludge degrading various substrates. Moreover, the location of calcium carbonate precipitation substantially affected the specific methanogenic activity (SMA) of the granular sludge. The SMA of the acetate-degrading biogranules dropped from 1.96 gCOD·gVSS·d to 0.61 gCOD·gVSS·d after 180-d of operation in the reactor. However, the SMA of the carbohydrate-degrading biogranules was not adversely affected by calcium carbonate precipitation.

关键词： calcium carbonate     precipitation     anaerobic granular sludge     wastewater composition     microstructure     upflow anaerobic sludge blanket (UASB)    

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Syngas composition study

Zhe WANG , Jinning YANG , Zheng LI , Yong XIANG ,

《能源前沿（英文）》 2009年 第3卷 第3期   页码 369-372 doi: 10.1007/s11708-009-0044-7

摘要： The syngas composition characteristic was investigated in the real slurry-feed gasifier using a detailed gas phase reaction mechanism. The results show that the time for syngas to reach equilibrium is much shorter than the residence time for slurry feed entrained-flow gasifiers, indicating a gas phase species partial equilibrium state. Further calculation shows that the four major species, CO, CO, H, and HO, are in equilibrium via the reaction Bayu PRABOWO,Herri SUSANTO,Kentaro UMEKI,Mi YAN,Kunio YOSHIKAWA. [J]. Front. Energy, 2015, 9(3): 362-370.Ashok Jayawant Rao KECHE, Gaddale AMBA PRASAD RAO. [J]. Front Energ, 2013, 7(3): 300-306.Souman RUDRA, H. T. KIM, Jinwook LEE, L. ROSENDAHL, . [J]. Front. Energy, 2010, 4(3): 402-413. Fuchen WANG , Xinwen ZHOU , Wenyuan GUO , Zhenghua DAI , Xin GONG , Haifeng LIU , Zunhong YU , . [J]. Front. Energy, 2009, 3(1): 117-122.

关键词： entrained-flow     gasifier     syngas    

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of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community

《环境科学与工程前沿（英文）》 2022年 第16卷 第11期 doi: 10.1007/s11783-022-1576-x

摘要：

● Presented coupled system enhanced biodegradation of antibiotic chloramphenicol.

关键词： Electrical stimulation     Biodegradation     Microbial community     Chloramphenicol    

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标题 作者 时间 类型 操作

Clinical factors associated with composition of lung microbiota and important taxa predicting clinicalprognosis in patients with severe community-acquired pneumonia

期刊论文

Effects of heavy rainfall on the composition of airborne bacterial communities

Gwang Il Jang, Chung Yeon Hwang, Byung Cheol Cho

期刊论文

Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

期刊论文

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Yadong YANG, Xiaomin FENG, Yuegao HU, Zhaohai ZENG

期刊论文

Impacts of backwashing on micropollutant removal and associated microbial assembly processes in sand filters

期刊论文

Distribution of phytoplankton community and its influence factors in an urban river network, East China

Ling Sun, Hui Wang, Yuanqing Kan, Shiliang Wang

期刊论文

Microbial responses to the use of NaClO in sediment treatment

期刊论文

Process stability and microbial community composition in pig manure and food waste anaerobic co-digesters

Conor Dennehy, Peadar G. Lawlor, Gillian E. Gardiner, Yan Jiang, Paul Cormican, Matthew S. McCabe, Xinmin Zhan

期刊论文

湿地芦苇根系微生物群落组成与环境应用

王东麟, 柏耀辉, 曲久辉

期刊论文

“Composition” and regularisation of architectural production in contemporary China

Hua LI

期刊论文

Calorie restriction and its impact on gut microbial composition and global metabolism

Xiaojiao Zheng, Shouli Wang, Wei Jia

期刊论文

Fungal diversity and its mechanism of community shaping in the milieu of sanitary landfill

期刊论文

Effect of wastewater composition on the calcium carbonate precipitation in upflow anaerobic sludge blanket

Shucheng YANG, Yanling HE, Charles CHOU, Pengxiang ZHANG, Dongqi WANG, Yonghong LIU,

期刊论文

Syngas composition study

Zhe WANG , Jinning YANG , Zheng LI , Yong XIANG ,

期刊论文

of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community

期刊论文