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N-糖基化 1

免疫球蛋白G 1

卵子发生 1

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女性性激素 1

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孕酮 1

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Adsorption and desorption of steroid hormones on saline soil

《环境科学与工程前沿（英文）》 2022年 第16卷 第11期 doi: 10.1007/s11783-022-1575-y

摘要：

● Organic matter content significantly affected adsorption of E2/EE2 on saline soil.

关键词： Saline soil     Steroid hormones     Adsorption     Desorption     Soil organic matter    

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Isolation and characterization of a bacterial strain that efficiently degrades sex steroid hormones

JI Shulan, LIU Zhipeng, REN Haiyan, LIU Zhipei

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 325-328 doi: 10.1007/s11783-007-0055-8

摘要： A bacterial strain, ZY3, growing on sex steroid hormones as the sole source of carbon and energy was isolated from the sewage treatment plant of a prophylactic steroids factory. ZY3 degrades the 3-methoxy-17-hydroxy-1,3,5(10),8(9)--4-estren (MHE). This strain was preliminarily identified as sp. ZY3 according to its morphology and its 16S rRNA gene sequence. During the experimental period (72 h), the optimum temperature, pH and 3-MHE concentration for the degradation of hydride by the strain ZY3 were 35vH, 10 and 10 mg/L, respectively. The degradation rate of the sex steroid hormones increased to 87% and 85% after the addition of maltose and peptone, respectively.

关键词： maltose     optimum temperature     prophylactic     3-methoxy-17-hydroxy-1     3     5     addition    

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Analyses of levels of thyroid hormones and its receptor expression in puerperants and newborns from an

JU Ying, CHEN Lan, JIANG Qi, YANG Kedi, CHEN Xuemin, XU Guojian, LI Liping

《医学前沿（英文）》 2008年 第2卷 第3期   页码 276-282 doi: 10.1007/s11684-008-0052-8

摘要： In this study, the serum levels, including thyroid hormones free triiodothyronine (FT), free thyroxine(FT),thyroid stimulating hormone(TSH)among the subjects from the exposed group ( = 48) and the control group ( = 45) were detected by immuno radiometric assay (IRMA). The expression levels of TR?1, TR?1, TSHR mRNA in placentas and umbilical cords were detected by fluorescent quantitative real-time PCR (FQ-PCR). The correlations between the thyroid hormone levels in maternal serum and umbilical serum, and between the expression levels of its receptors mRNA in placentas and umbilical cords were determined. We found that the FT levels of both maternal serum and umbilical cord serum in the exposed group were lower than those in the control ( < 0.05). However, the increased TSH levels in the exposed group had statistically significance compared to those in the control group ( < 0.05). The TR?1 and TR?1 mRNA levels both in placentas and umbilical cords in the exposed group were lower than those in the control group ( < 0.05 and 0.01). However, the TSHR mRNA levels in the exposed group were significantly different compared to those in the control group ( < 0.01). The serum FT4 and TSH levels of parturient women were positively correlated with those of the newborns in both groups ( < 0.05 and 0.01). The mRNA levels of TR?1, TR?1 and TSHR in the placentas were positively correlated with those in umbilical cords in both groups ( < 0.01). The findings suggest that some environmental pollutants existing in the electronic waste (e-waste) dismantling region may affect the health of local parturient women and newborns, representing changes both in serum levels of thyroid hormones and in mRNA expression of its receptors.

关键词： control     immuno radiometric     FT     TSH     exposed    

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Effect of biochar amendment on soil’s retention capacity for estrogenic hormones from poultry manure

Sukhjot MANN, Zhiming QI, Shiv O. PRASHER, Lanhai LI, Dongwei GUI, Qianjing JIANG

《农业科学与工程前沿（英文）》 2017年 第4卷 第2期   页码 208-219 doi: 10.15302/J-FASE-2017138

摘要： Most animals, including humans, produce natural sex hormones such as estrogens: 17β-estradiol (E2) and estrone (E1). These compounds are able to disrupt the reproductive systems of living organisms at trace concentrations (ng·L ). This experiment tests the hypothesis that 1% slow pyrolysis biochar-amended sandy soil could retain significant amount of estrogens (E1, E2) from poultry manure in its second year of application. The experiment was conducted over 46 days and consisted of a series of lysimeters containing sandy soil with biochar-amended topsoil. The application rate of poultry manure was kept at 2.47 kg·m . The biochar held a significant concentration of hormone during the first year of its application. However, in the following year (current study), there was no significant retention of hormones in the biochar-amended soil. During the first year after application, the biochar was fresh, so its pores were available for hydrophobic interactions and held significant concentration of hormones. As time passed there were several biotic and abiotic changes on the surface of the biochar so that after some physical fragmentation, pores on the surface were no longer available for hydrophobic interactions. The biochar started releasing dissolved organic carbon, which facilitated greater mobility of hormones from poultry manure down the soil profile.

关键词： adsorption     degradation     dissolved organic carbon     17β-estradiol (E2)     estrone (E1)    

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美洲鲥鱼卵巢发育规律和性类固醇激素变化研究

洪磊,李兆新,陈超,郭正龙,王蔚芳,雷霁霖

《中国工程科学》 2014年 第16卷 第9期   页码 86-92

摘要：

采用组织学、形态学及电化学等技术方法，研究了人工养殖条件下美洲鲥鱼雌性亲鱼卵巢发育和性类固醇激素的周期变化规律。结果表明，美洲鲥鱼属于非同步分批产卵类型；根据卵巢外部形状大小及色泽等的不同，可将美洲鲥鱼卵巢发育过程分为6 个时期；根据卵母细胞的形态结构、卵黄物质的积累和滤泡细胞的变化，将卵子发生划分为6 个时相；卵巢周期变化过程中，肝脏指数（HSI）和肥满度（CF）变化不显著，性腺指数（GSI）与血浆中雌二醇（E2）水平表现出与性腺发育协同一致的变化规律。

关键词： 美洲鲥鱼     卵巢发育     卵子发生     组织学     形态学     性类固醇激素    

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Ablation of steroid receptor coactivator-3 in mice impairs adipogenesis and enhances energy expenditure

Ling-Yan XU PhD, Xin-Ran MA PhD, Xiao-Ying LI PhD, MD, Shu WANG PhD, Guang NING PhD, MD, Jie-Li LI PhD, Jian-Ming XU PhD,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 229-234 doi: 10.1007/s11684-010-0028-3

摘要： Obesity is a medical condition in which excess body fat has accumulated to an extent and may have an adverse effect on health, leading to reduced life expectancy, impaired energy homeostasis and increased health problems. The p160 steroid receptor coactivator (SRC) gene family members have been suggested to be involved in energy homeostasis, but the impact of SRC-3 ablation on white and brown adipose tissue needs to be elucidated. In the current study, we collected data and carried out morphological studies on the effect of SRC-3 deficiency on white adipose tissue (WAT) and brown adipose tissue (BAT). Primary cells were cultured to investigate the differentiation ability of both adipocytes. Western blot was applied to detect the expression of master genes governing adipogenesis and thermogenesis. We observed that SRC-3 mice were lean, with reduced WAT and decreased serum leptin levels, mainly due to the smaller white adipocyte size caused by impaired adipogenesis, presented by decreased peroxisome proliferator activated receptor (PPAR) expression. In the BAT, the lipid droplets decreased significantly in SRC-3 mice as demonstrated by histological analysis and electron microscopic observation, which could be explained by enhanced thermogenesis. The expression of thermogenic marker gene PPAR coactivator 1α and uncoupling protein-1 increased in BAT of SRC-3 mice, which proved our observations. Collectively, these results demonstrate that SRC-3 plays a key role in adipogenesis and energy expenditure.

关键词： steroid receptor coactivator-3     white adipose tissue     brown adipose tissue     obesity     adipocytes     energy expenditure    

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INTERACTIONS BETWEEN ABOVE AND BELOW GROUND PLANT STRUCTURES: MECHANISMS AND ECOSYSTEM SERVICES

《农业科学与工程前沿（英文）》 2022年 第9卷 第2期   页码 197-213 doi: 10.15302/J-FASE-2021433

摘要：

Interactions between above and below ground parts of plants can be considered under the (overlapping) categories of energy, material and information. Solar energy powers photosynthesis and transpiration by above ground structures, and drives most water uptake through roots and supplies energy as organic matter to below ground parts, including diazotrophic symbionts and mycorrhizas. Material transfer occurs as water and dissolved soil-derived elements transport up the xylem, and a small fraction of water moving up the xylem with dissolved organic carbon and other solutes down the phloem. The cytosolic nature of sieve tubes accounts for at least some of the cycling of K, Mg and P down the phloem. NO3– assimilation of above ground parts requires organic N transport down phloem with, in some cases, organic anions related to shoot acid-base regulation. Long-distance information transfer is related development, biotic and abiotic damage, and above and below ground resource excess and limitation. Information transfer can involve hydraulic, electrical and chemical signaling, with their varying speeds of transmission and information content. Interaction of above and below ground plant parts is an important component of the ecosystem service of storing atmospheric CO2 as organic C in soil, a process that has decreased since the origin of agriculture.

关键词： aerenchyma / carbon accumulation / hormones / phloem / xylem    

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Role of water chemistry on estrone removal by nanofiltration with the presence of hydrophobic acids

Xue JIN,Jiangyong HU

《环境科学与工程前沿（英文）》 2015年 第9卷 第1期   页码 164-170 doi: 10.1007/s11783-014-0747-9

摘要： Hydrophobic acid organic matter (HpoA) extracted from treated effluent has been known to improve the rejection of steroid hormone estrone by reverse osmosis (RO) and nanofiltration (NF) membranes. In this study, the effects of solution chemistry (solution pH and ionic strength) on the estrone rejection by NF membrane with the presence of HpoA were systematically investigated. Crossflow nanofiltration experiments show that the presence of HpoA significantly improved estrone rejection at all pH and ionic strength levels investigated. It is consistently shown that the “enhancement effect” of HpoA on estrone rejection at neutral and alkaline pH is attributed to the binding of estrone by HpoA macromolecules via hydrogen bonding between phenolic functional groups in feed solutions, which leads to an increase in molecular weight and appearance of negative charge. The membrane exhibited the best performance in terms of estrone rejection at pH 10.4 (compared to pH 4 and pH 7) as a result of strengthening the electrostatic repulsion between estrone and membrane with the presence of HpoA. At neutral pH level, the ability of HpoA macromolecules to promote estrone rejection became stronger with increasing ionic strength due to their more extended conformation, which created more chances for the association between estrone and HpoA. The important conclusion of this study is that increasing solution pH and salinity can greatly intensify the “enhancement effect” of HpoA. These results can be important for NF application in direct/indirect potable water reuse.

关键词： indirect potable water reuse     steroid hormone     nanofiltration(NF)     rejection     water chemistry     hydrophobic acids    

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月经周期中免疫球蛋白G N-糖基化的周期性变化 Article

Julija Jurić, Hongli Peng, Manshu Song, Frano Vučković, Jelena Šimunović, Irena Trbojević-Akmačić, Youxin Wang, Jiaonan Liu, Qing Gao, Hao Wang, Qiaoyun Chu, Marija Pezer, Wei Wang, Gordan Lauc

《工程（英文）》 2023年 第26卷 第7期   页码 108-118 doi: 10.1016/j.eng.2022.10.020

摘要：

免疫球蛋白G（IgG）是最丰富的血浆糖蛋白，也是重要的体液免疫介质。聚糖组成影响IgG 对配体的亲和力以及随之而来的免疫反应。IgG N-糖基化的修饰被认为是性激素调节免疫系统的各种机制之一。虽然月经周期是大多数育龄妇女中与性激素相关的中枢生理过程，但月经周期中的IgG N-糖基化动力学尚未得到研究。为了填补这一空白，本研究使用亲水相互作用超高效液相色谱（HILIC-UPLC）分析了70 名绝经前健康妇女在月经周期的12 个时间点（每7 天一次，持续3 个月）的血浆IgG N-聚糖。研究观察到IgG N-糖基化的周期性变化与月经周期阶段和血浆中的性激素浓度有关。在综合队列水平上，每种性状的模拟平均月经周期对IgG N-糖基化性状丰度的影响较低，无半乳糖基化N-聚糖的峰值为1.1%。然而，在某些情况下，个体自身变化相对较高；例如，月经周期中唾液酸化N-聚糖的最小值和最大值之间的最大差异高达21%。在所有测量中，月经周期阶段可以解释单半乳糖基化的单个IgG 糖基化性状丰度高达0.72%的变化。相比之下，高达99%的双半乳糖基化丰度变化可归因于IgG N-糖基化的个体差异。总之，月经周期中发生的IgG N-聚糖模式变化的平均程度很小；因此，无论月经周期阶段如何，都可以在大样本量研究中对女性进行IgG N-聚糖谱分析。

关键词： N-糖基化     免疫球蛋白G     月经周期     女性性激素     雌激素     孕酮     睾酮     妇女    

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标题 作者 时间 类型 操作

Adsorption and desorption of steroid hormones on saline soil

期刊论文

Isolation and characterization of a bacterial strain that efficiently degrades sex steroid hormones

JI Shulan, LIU Zhipeng, REN Haiyan, LIU Zhipei

期刊论文

Analyses of levels of thyroid hormones and its receptor expression in puerperants and newborns from an

JU Ying, CHEN Lan, JIANG Qi, YANG Kedi, CHEN Xuemin, XU Guojian, LI Liping

期刊论文

Effect of biochar amendment on soil’s retention capacity for estrogenic hormones from poultry manure

Sukhjot MANN, Zhiming QI, Shiv O. PRASHER, Lanhai LI, Dongwei GUI, Qianjing JIANG

期刊论文

美洲鲥鱼卵巢发育规律和性类固醇激素变化研究

洪磊,李兆新,陈超,郭正龙,王蔚芳,雷霁霖

期刊论文

Ablation of steroid receptor coactivator-3 in mice impairs adipogenesis and enhances energy expenditure

Ling-Yan XU PhD, Xin-Ran MA PhD, Xiao-Ying LI PhD, MD, Shu WANG PhD, Guang NING PhD, MD, Jie-Li LI PhD, Jian-Ming XU PhD,

期刊论文

INTERACTIONS BETWEEN ABOVE AND BELOW GROUND PLANT STRUCTURES: MECHANISMS AND ECOSYSTEM SERVICES

期刊论文

Role of water chemistry on estrone removal by nanofiltration with the presence of hydrophobic acids

Xue JIN,Jiangyong HU

期刊论文

月经周期中免疫球蛋白G N-糖基化的周期性变化

Julija Jurić, Hongli Peng, Manshu Song, Frano Vučković, Jelena Šimunović, Irena Trbojević-Akmačić, Youxin Wang, Jiaonan Liu, Qing Gao, Hao Wang, Qiaoyun Chu, Marija Pezer, Wei Wang, Gordan Lauc

期刊论文