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can promote cucumber growth and control cucumber corynespora leaf spot

《农业科学与工程前沿（英文）》 doi: 10.15302/J-FASE-2024537

摘要：

● First report of bacteria Paenarthrobacter nitroguajacolicus for effective control of cucumber corynespora leaf spot and promotion of cucumber growth.

关键词： Adaptability     antagonistic bacterium     disease resistance     growth promotion     secondary metabolite    

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Mechanical design, modeling, and identification for a novel antagonistic variable stiffness dexterous

《机械工程前沿（英文）》 2022年 第17卷 第3期 doi: 10.1007/s11465-022-0691-5

摘要： This study traces the development of dexterous hand research and proposes a novel antagonistic variable stiffness dexterous finger mechanism to improve the safety of dexterous hand in unpredictable environments, such as unstructured or man-made operational errors through comprehensive consideration of cost, accuracy, manufacturing, and application. Based on the concept of mechanical passive compliance, which is widely implemented in robots for interactions, a finger is dedicated to improving mechanical robustness. The finger mechanism not only achieves passive compliance against physical impacts, but also implements the variable stiffness actuator principle in a compact finger without adding supererogatory actuators. It achieves finger stiffness adjustability according to the biologically inspired stiffness variation principle of discarding some mobilities to adjust stiffness. The mechanical design of the finger and its stiffness adjusting methods are elaborated. The stiffness characteristics of the finger joint and the actuation unit are analyzed. Experimental results of the finger joint stiffness identification and finger impact tests under different finger stiffness presets are provided to verify the validity of the model. Fingers have been experimentally proven to be robust against physical impacts. Moreover, the experimental part verifies that fingers have good power, grasping, and manipulation performance.

关键词： multifingered hand     mechanism design     robot safety     variable stiffness actuator    

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Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the

Bingjie Xu, Gang Xue, Xing Yang

《环境科学与工程前沿（英文）》 2018年 第12卷 第5期 doi: 10.1007/s11783-018-1080-5

摘要：

• An ibuprofen-degrading strain, Serratia marcescens BL1, was isolated and identified.

• The effects of various factors on ibuprofen degradation by BL1 were evaluated.

• Strain BL1 was applied to a laboratory-scale biological aerated filter system.

• Strain BL1 was stable in both static tests and in the biological aerated filter system.

关键词： Ibuprofen     Biological aerated filter     Degrading bacterium     Serratia marcescens    

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A heterotrophic nitrification-aerobic denitrification bacterium TJPU05 suitable for nitrogen removal

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1503-6

摘要：

• H. venusta TJPU05 showed excellent HN-AD ability at high salinity.

关键词： Salt-tolerant bacteria     H. venusta TJPU05     Heterotrophic nitrification and aerobic denitrification     High-salinity wastewater    

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In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

《环境科学与工程前沿（英文）》 2014年 第8卷 第1期   页码 69-78 doi: 10.1007/s11783-013-0502-7

摘要： Effluents from wastewater treatment plant (WWTP) have been reported to have a broad spectrum of endocrine disrupting compounds (EDCs). The majority of studies have focused on the occurrence of estrogenic activity, while ignoring nuclear hormone receptors (NRs) pathways. In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic and agonistic effects on estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR), estrogen-related receptor (ERR) and aryl hydrocarbon receptor (AHR), were conducted to evaluate the removal efficiencies of EDCs by different treatment processes of a WWTP located in Beijing. Estrogenic, anti-estrogenic, anti- androgenic, anti-progesteronic, anti-ERR and the activation of AHR activities were detected in samples from all treatment processes and the receiving water. The concentration of estrogenic contaminants with estradiol (E ) equivalent concentrations ranged from 0.82 × 10 to 3.54 × 10 g E -EQ·L . The concentration of anti-estrogenic contaminants with 4-hydroxytamoxifen (4-OHT) equivalent concentrations ranged from 1.24 × 10 to 2.36 × 10 g 4-OHT-EQ·L . The concentration of anti-androgenic contaminants ranged from 2.21 × 10 to 3.52 × 10 g flutamide-EQ·L . The concentration of anti-progesteronic contaminants ranged from 3.15 × 10 to 2.71 × 10 g RU486-EQ·L . The concentration of anti-ERR contaminants ranged from 7.09 × 10 to 6.50 × 10 g 4-OHT-EQ·L . The concentration of AHR activators ranged from 1.7 × 10 to 3.4 × 10 g TCDD-EQ·L . These processes including secondary clarifier, coagulation, as well as coal and sand filtration could eliminated 67.2% of estrogenic contaminants, 47.0% of anti-estrogenic contaminants, 98.3% of anti-androgenic contaminants, 88.4% of anti-progesteronic contaminants, 65.4% of anti-ERR contaminants and 46.9% of AHR activators. WWTP effluents contain multiple receptor disruptors may have very complex adverse effects on exposed organisms.

关键词： waste water     in vitro     recombinant yeast assay     ethoxyresorufin-O-deethylase (EROD)     receptor     endocrine    

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Studies on culture condition of new marine bacterium sp. SY01

LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua

《化学科学与工程前沿（英文）》 2008年 第2卷 第4期   页码 443-446 doi: 10.1007/s11705-008-0065-5

摘要： New marine bacterium sp. SY01, producer of prodigiosin, was isolated from the seawaters of Sanya Bay. The culture conditions of this bacterium were investigated. sp. SY01 was cultured in 2216E media which contained tryptophan, histidine, lactonic acid, camphor, limonene, casein, diphenyl guanidine, coumarin and 1,3-dinitrobenzene, respectively. After 5 days cultivation, the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode. As the results, tryptophan, histidine and casein didn’t show any observable influences on the biosynthesis of prodigiosin. Lactonic acid, camphor, limonene, diphenyl guanidine, coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a certain extent, slower the culture broth to turn red. However, 1, 3-dinitrobenzene inhibited the bacteria to produce prodigiosin completely. MS data suggested that various metabolites with chemodiversity were produced in different culture media. In particular, a series of high-molecular-weight compounds with high relative abundances were observed in the medium containing limonene. To further optimize the culture condition, more new prodigiosin analogues and lead compounds can be obtained and the goal of “one strain-many compounds” can be achieved.

关键词： producer     high-molecular-weight     relative     culture     different culture    

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LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1349-3

摘要： Abstract • Pseudomonas mendocina was first reported for aerobic nitrate removal. • It removed 90% of NO3−-N in 24 h under aerobic conditions. • This strain converted NO3−-N to bio-nitrogen (37.9%) and gaseous nitrogen (49.7%). • Inoculation of this strain increased sludge denitrification rate by 4.3 times. The problem of nitrate accumulation in aerobic tank and total nitrogen excessive discharge in effluent was very common in traditional livestock and poultry farming wastewater treatment systems owing to the lengthy process flow and low process control level. A strain LYX of aerobic bacterium was isolated from the activated sludge of a wastewater treatment system in a pig farm, which could remove nitrate effectively in aerobic tank and was identified Pseudomonas mendocina by 16S rRNA sequencing. Under the condition of nitrate as the sole nitrogen source, this strain removed over 90% of NO3−-N with an initial concentration of 110 mg/L under aerobic conditions within 48 hours. Among them, 37.9% of NO3−-N was assimilated into Bio-N, about 51.9% was reduced to gaseous nitrogen and less than 0.5% of nitrogen was replaced by NO2−-N and NH4+-N, 9.7% NO3−-N remained in the effluent at the end. At the same time, four key genes (napA, nirK, norB and nosZ) related to nitrate nitrogen removal were expressed during the denitrification process of P. mendocina LYX, in which the transcription level of the indicator genes of this aerobic denitrifying bacterium (napA) was the highest. In addition, it was found with the 15N tracer technique that inoculation of this strain on sludge increased the amount of nitrogen loss from 9.26 nmol N/(g·h) to 23.835 nmol N/(g·h). Therefore, P. medocina LYX is a potential bioagent for advanced nitrogen removal by assimilating and reducing nitrate simultaneously in aerobic tanks.

关键词： Pseudomonas mendocina     Aerobic nitrate removal     15N tracing technique     Denitrification assimilatively and disimilatively     Aerobic denitrifying genes    

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Pollution and biodegradation of hexabromocyclododecanes: A review

Ling Huang, Syed Bilal Shah, Haiyang Hu, Ping Xu, Hongzhi Tang

《环境科学与工程前沿（英文）》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1190-8

摘要： • Bioremediation is the most cost-effective approach for degradation of HBCDs. • Bacteria or bacterial consortia are used in the cases of bio-augmentation. • Microbes combined with phytoremediation increase the remediation efficiency. Hexabromocyclododecanes (HBCDs) are the most common brominated flame-retardants after polybrominated diphenyl ethers. HBCDs can induce cancer by causing inappropriate antidiuretic hormone syndrome. Environmental contamination with HBCDs has been detected globally, with concentrations ranging from ng to mg. Methods to degrade HBCDs include physicochemical methods, bioremediation, and phytoremediation. The photodegradation of HBCDs using simulated sunlight or ultraviolet lamps, or chemical catalysts are inefficient and expensive, as is physicochemical degradation. Consequently, bioremediation is considered as the most cost-effective and clean approach. To date, five bacterial strains capable of degrading HBCDs have been isolated and identified: Pseudomonas sp. HB01, Bacillus sp. HBCD-sjtu, Achromobacter sp. HBCD-1, Achromobacter sp. HBCD-2, and Pseudomonas aeruginosa HS9. The molecular mechanisms of biodegradation of HBCDs are discussed in this review. New microbial resources should be explored to increase the resource library in order to identify more HBCD-degrading microbes and functional genes. Synthetic biology methods may be exploited to accelerate the biodegradation capability of existing bacteria, including modification of the degrading strains or functional enzymes, and artificial construction of the degradation microflora. The most potentially useful method is combining micro-degradation with physicochemical methods and phytoremediation. For example, exogenous microorganisms might be used to stimulate the adsorption capability of plants for HBCDs, or to utilize an interaction between exogenous microorganisms and rhizosphere microorganisms to form a new rhizosphere microbial community to enhance the biodegradation and absorption of HBCDs.

关键词： Hexabromocyclododecane     Biodegradation     Bioremediation     Phytoremediation     Bacterium    

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Screening of potential aquatic probiotics from the major microflora of guppies (

Aparna BALAKRISHNA, T. R. KEERTHI

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 163-173 doi: 10.1007/s11705-012-1283-4

摘要： The fish ( ) was used as the source for probiotics. 46 bacterial isolates were obtained from the skin, gills, guts and intestines of the guppy, (collected from a government model fish farm in Kottayam, India). Of the above isolated strains, four isolates were selected based on their inhibitory spectrum against five indicator strains, 1739, 3906, 2495, 1271 and 1424 (standard cultures collected from Microbial Type Culture Collection (MTCC) Chandigarh, India). Among the resulting isolates, two were gram-positive cocci, namely MBTU-PB2 and MBTU-PB3 and belong to the genus . The other two were gram-negative rods, namely MBTU-PB1 and MBTU-PB4, of the genera and , respectively. The basic probiotic characteristics of these isolates such as the production of bacteriocin like inhibitory substances (BLIS), antibiotic sensitivities and growth profiles were also determined. The above four isolated strains exhibited different antagonisms than the five indicator strains. During incubation, the antibacterial activity gradually increased in the inhibition zone and was influenced by the lag period ( ) and doubling time. The lag periods for most of the four selected strains were shorter than those of the indicator strains and the isolates had different growth rates ( ) than the indicator strains. All four isolates produced BLIS, however, the strains had different BLIS activities against the indicator strains. Treatment of the neutralized cell free supernatants of the selected isolates with proteases eliminated or reduced the BLIS activity, suggesting a proteinaceous nature of the inhibitory compounds. Further, the optimum BLIS activity was observed at neutral pH after 18 h of incubation. The antibiotic sensitivity assay revealed that the isolates were susceptible to routinely used antibiotics, whereas the plasmid profiles showed that the plasmids had no role in the antagonistic properties of the four isolated strains. The results showed that the isolates could be a promising source for biocontrol agents in aquacultures.

关键词： aquaculture     normal flora     immunomodulatory effect     probiotic     antagonistic principle     bacteriocin like inhibitory substances    

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Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG,

《化学科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 393-398 doi: 10.1007/s11705-009-0252-z

摘要： -psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of -psicose from -fructose. In this paper, another bacterium which could convert -fructose to -psicose was isolated and identified as . The process parameters of -psicose production using permeabilized cells of SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10min, and reaction conditions: cell concentration, 30g dry cell wt/L; concentration of substrate, 50g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5g/L -psicose with a -psicose productivity of 0.82g·L·h. This is the first report of bioproduction of -psicose using permeabilized cells of .

关键词： reaction     permeabilized     substrate     bacterium     process    

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Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter sphaeroides AR-3

Guanghong ZHENG, Zhuhui KANG, Yifan QIAN, Lei WANG,

《环境科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 387-392 doi: 10.1007/s11783-009-0154-9

摘要： Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ. [J]. Front. Environ. Sci. Eng., 2016, 10(3): 513-521.Yongtao LV,Xuan CHEN,Lei WANG,Kai JU,Xiaoqiang CHEN,Rui MIAO,Xudong WANG. [J]. Front. Environ. Sci. Eng., 2016, 10(2): 390-398.Zulkifly JEMAAT,Josep Anton TORA,Albert BARTROLI,Julián CARRERA,Julio PEREZ. [J]. Front. Environ. Sci. Eng., 2015, 9(3): 528-533.Wei LI, Xiaowen DING, Min LIU, Yuewen GUO, Lei LIU. [J]. Front Envir Sci Eng, 2012, 6(6): 892-900.Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO. [J]. Front Envir Sci Eng, 2012, 6(1): 125-130.Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN. [J]. Front Envir Sci Eng Chin, 2011, 5(4): 519-525.Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG. [J]. Front Envir Sci Eng Chin, 2011, 5(2): 291-297.Yanhui ZHAN, Jianwei LIN, Yanling QIU, Naiyun GAO, Zhiliang ZHU. [J]. Front Envir Sci Eng Chin, 2011, 5(1): 65-75.Rongchang WANG, Xinmin ZHAN, Yalei ZHANG, Jianfu ZHAO. [J]. Front Envir Sci Eng Chin, 2011, 5(1): 48-56.Bo WANG, Wei WAN, Jianlong WANG, . [J]. Front.Environ.Sci.Eng., 2009, 3(4): 380-386.GUO Jinsong, YANG Guohong, FANG Fang, QIN Yu. [J]. Front.Environ.Sci.Eng., 2008, 2(4): 439-445.GENG Bing, ZHU Yanfang, JIN Zhaohui, LI Tielong, KANG Haiyan, WANG Shuaima. [J]. Front.Environ.Sci.Eng., 2007, 1(3): 357-361.WU Deyi, HU Zhanbo, WANG Xinze, HE Shengbing, KONG Hainan. [J]. Front.Environ.Sci.Eng., 2007, 1(2): 213-220.XU Zhengyong, YANG Zhaohui, ZENG Guangming, XIAO Yong, DENG Jiuhua. [J]. Front.Environ.Sci.Eng., 2007, 1(1): 43-48.

关键词： ammonium     anoxygenic phototrophic bacterium     biohydrogen     glutamine auxotrophic     tofu wastewater    

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标题 作者 时间 类型 操作

can promote cucumber growth and control cucumber corynespora leaf spot

期刊论文

Mechanical design, modeling, and identification for a novel antagonistic variable stiffness dexterous

期刊论文

Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the

Bingjie Xu, Gang Xue, Xing Yang

期刊论文

A heterotrophic nitrification-aerobic denitrification bacterium TJPU05 suitable for nitrogen removal

期刊论文

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

期刊论文

Studies on culture condition of new marine bacterium sp. SY01

LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua

期刊论文

LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

期刊论文

Pollution and biodegradation of hexabromocyclododecanes: A review

Ling Huang, Syed Bilal Shah, Haiyang Hu, Ping Xu, Hongzhi Tang

期刊论文

Screening of potential aquatic probiotics from the major microflora of guppies (

Aparna BALAKRISHNA, T. R. KEERTHI

期刊论文

Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

Longtao ZHANG, Bo JIANG, Wanmeng MU, Tao ZHANG,

期刊论文

Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter sphaeroides AR-3

Guanghong ZHENG, Zhuhui KANG, Yifan QIAN, Lei WANG,

期刊论文