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期刊论文 7

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2023 1

2022 1

2020 2

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脂肪酶 2

sn-2棕榈酸甘油酯 1

丝孢堆黑粉菌 1

乙酰肝素酶 1

人乳替代脂 1

分子动力学模拟 1

固体酸碱 1

均相酸碱 1

定点突变 1

定量分析 1

心脏代谢 1

棕榈酸 1

理性设计 1

生物柴油 1

糖尿病性心肌病 1

结构甘油三酯 1

脂蛋白脂肪酶 1

莫西沙星 1

血管内皮生长因子 1

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Study of ultrasound-promoted, lipase-catalyzed synthesis of fructose ester

Kai ZHU, Hui LIU, Pingfang HAN, Ping WEI,

《化学科学与工程前沿(英文)》 2010年 第4卷 第3期   页码 367-371 doi: 10.1007/s11705-009-0312-4

摘要: The effect of low energy ultrasound in biochemistry and biotechnology has attracted great attention in recent years. It can enhance substrate dissolution and improve mass transfer within and outside of a cell, both of which are beneficial to the synthesis of fructose ester. Here we describe the experimental study of the effect of ultrasounds of different intensity on the lipase-catalyzed synthesis of fructose ester in the solvent butanone. The results were compared with control reactions performed with no ultrasound. High performance liquid chromatography (HPLC) and thin layer chromatography (TLC) were used for qualitative and quantitative analyses. The results show the following: 1) the concentration of mono-ester and diester increased with the reaction time, either with or without ultrasonic irradiation. Low energy ultrasound accelerated the reaction due to the effect of ultrasonic steady cavitations, and high energy ultrasound was not beneficial to the reaction. 2) The application of ultrasound played an important role in our lipase-catalyzed reaction. It decreased reaction time as compared to a reaction without ultrasound that resulted in the same yield, increased reaction rate, and enhanced the amount of fructose ester produced. When the frequency was 10 kHz and sound intensity was 0.16 W·m, the concentration of ester was twofold more than without ultrasonic irradiation after a reaction time of up to 12 h. With the proper ultrasonic parameters, the overall concentration of production appeared to increase exponentially with the reaction time. 3) Ultrasound had little effect on the initial reaction rate, and continuous ultrasonic irradiation was favorable for the reaction. The longer the reaction continued, the more obvious the effects of the ultrasound became in our experiments.

Regioselective acylation of pyridoxine catalyzed by immobilized lipase in ionic liquid

BAI Shu, REN Mengyuan, WANG Lele, SUN Yan

《化学科学与工程前沿(英文)》 2008年 第2卷 第3期   页码 301-307 doi: 10.1007/s11705-008-0060-x

摘要: The regioselective acylation of pyridoxine catalyzed by immobilized lipase () in 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF) has been investigated, and compared with that in acetonitrile (ACN). The acetylation of pyridoxine using acetic anhydride in [BMIM]PF gave comparable conversion of pyridoxine to 5-monoacetyl pyridoxine with considerably higher regioselectivity (93%–95%) than that in ACN (70%–73%). Among the tested parameters, water activity () and temperature have profound effects on the reaction performances in either [BMIM]PF or ACN. For the reaction in [BMIM]PF, higher temperature (50°C–55°C) and lower (<0.01) are preferable conditions to obtain better conversion and regioselectivity. Mass transfer limitation and intrinsic kinetic from the ionic nature of ionic liquids (ILs) may account for a different rate-temperature profile and a lower velocity at lower temperature in [BMIM]PF-mediated reaction. Moreover, consecutive batch reactions for enzyme reuse also show that lipase exhibited a much higher thermal stability and better reusability in [BMIM]PF than in ACN, which represents another advantage of ILs as an alternative to traditional solvents beyond green technology.

关键词: ACN     stability     1-butyl-3-methylimidazolium hexafluorophosphate     different rate-temperature     alternative    

糖尿病发作后心脏脂蛋白脂肪酶的变化 Review

Chae Syng Lee, Yajie Zhai, Brian Rodrigues

《工程(英文)》 2023年 第20卷 第1期   页码 19-25 doi: 10.1016/j.eng.2022.06.013

摘要:

由于心脏持续地收缩和舒张,需要大量的能量,其中脂肪酸(FA)是其三磷酸腺苷(ATP)的主要来源。但是,心脏无法制造这种底物,而是从多种来源获得脂肪酸,包括通过脂蛋白脂肪酶(LPL)的作用。脂蛋白脂肪酶在心肌细胞中产生,随后分泌到质膜上的硫酸乙酰肝素蛋白聚糖(HSPG)结合位点。然后为了将脂蛋白脂肪酶转移到内皮细胞管腔,糖基磷脂酰肌醇锚定的高密度脂蛋白结合蛋白1(GPIHBP1)与间质性脂蛋白脂肪酶结合,并将其转移到血管管腔,在那里脂蛋白脂肪酶可将循环中的甘油三酯分解为脂肪酸。内源性-β-葡萄糖醛酸酶乙酰肝素酶(Hpa)的独特之处在于,它是唯一已知的哺乳动物酶,可以裂解硫酸乙酰肝素,从而促进上述脂蛋白脂肪酶从心肌细胞HSPG中释放。在糖尿病中,一直认为心脏产生能量方式的改变是导致糖尿病性心肌病(DCM)的原因。糖尿病发展到中度后,随着葡萄糖利用率的降低,由于Hpa 作用的增强,心脏血管腔内的脂蛋白脂肪酶活性得到增强。虽然这种适应可能有助于补偿心脏对葡萄糖的利用不足,但从长期来看,它是具有毒性的,因为有害的脂质代谢物积聚,以及脂肪酸氧化增强和因此造成的氧化应激,最终导致细胞死亡。这与一种心脏保护生长因子——血管内皮生长因子B(VEGFB)的丧失同时发生。本文探讨了乙酰肝素酶、脂蛋白脂肪酶和血管内皮生长因子B之间的相互联系及其在糖尿病性心肌病中的潜在影响。鉴于缺乏基于机制的DCM治疗,了解这种心肌病的病理,以及脂蛋白脂肪酶的作用,将有助于我们推进其临床治疗。

关键词: 心脏代谢     脂蛋白脂肪酶     乙酰肝素酶     血管内皮生长因子     糖尿病性心肌病    

Expression and characterization of a CALB-type lipase from

Jiang-Wei Shen, Xue Cai, Bao-Juan Dou, Feng-Yu Qi, Xiao-Jian Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

《化学科学与工程前沿(英文)》 2020年 第14卷 第5期   页码 868-879 doi: 10.1007/s11705-019-1889-x

摘要: A lipase from SRZ2 (SRL) with 73% amino acid sequence identity to lipase B (CALB) was cloned and overexpressed in . The recombinant SRL showed a preference for short-chain -nitrophenyl esters. It achieved maximum activity at pH 8.0 and 65°C for -nitrophenyl hexanoate (C6) with and / values of 0.14 mmol∙L and 1712 min ∙mmol∙L at 30°C, respectively. SRL displayed excellent thermostability and pH stability, retaining more than 79% of its initial activity after incubation at 60°C for 72 h and 75% at pH 3 to 11 for 72 h. It also maintained most of its activity in the presence of inhibitors and detergents except sodium dodecyl sulfate, and it tolerated organic solvents. SRL was covalently immobilized and successfully used for ethyl hexanoate synthesis in cyclohexane or in a solvent-free system with a high conversion yield (>95%). Furthermore, high conversion yield was also achieved for the synthesis of various short-chain flavor esters when high substrate concentrations of 2 mol∙L were applied. This study indicated that a CALB-type lipase from SRZ2 showed great potential in organic ester synthesis.

关键词: lipase     Sporisorium reilianum     biochemical characterization     short-chain flavor ester     solvent-free system    

酶法合成sn-2棕榈酸甘油酯的研究进展 Review

韦伟, 孙聪, 王小三, 金青哲, Xuebing Xu, Casimir C. Akoh, 王兴国

《工程(英文)》 2020年 第6卷 第4期   页码 406-414 doi: 10.1016/j.eng.2020.02.008

摘要:

人乳脂(human milk fat, HMF)是婴儿重要的能量和营养来源,甘油三酯(TAG)在人乳脂中的含量约为98%,其具有独特的分子结构。HMF在甘油碳骨架sn-2位的棕榈酸(PA)含量超过70%,而在sn-1,3位高度富集多不饱和脂肪酸。研究表明,HMF中特定的TAG结构在婴儿生长过程中起重要作用。Sn-2棕榈酸甘油酯是一种结构TAG,主要由1,3-二油酸-2-棕榈酸甘油三酯组成,将其添加到婴幼儿配方奶粉中可以模拟HMF的TAG结构。本文综述了过去25年中酶法合成sn-2棕榈酸甘油酯的研究进展及其在实验室条件下的制备流程,重点分析了结构TAG合成过程中所用的商业化sn-1,3位选择性脂肪酶、TAG结构分析的常用方法,以及一些市售sn-2棕榈酸甘油酯产品。另外,本文还对酶法合成sn-2棕榈酸甘油酯的前景进行了展望。

关键词: 结构甘油三酯     棕榈酸     人乳替代脂     酶法合成反应     sn-2棕榈酸甘油酯     定量分析    

理性设计大幅提高一种丝孢堆黑粉菌来源脂肪酶催化活性——用于合成莫西沙星手性中间体 Article

蔡雪, 沈江伟, 强煜, 华婧, 马樟奇, 柳志强, 郑裕国

《工程(英文)》 2022年 第19卷 第12期   页码 207-216 doi: 10.1016/j.eng.2022.03.020

摘要:

通过脂肪酶不对称拆分外消旋N-乙酰基-哌啶-2,3-二甲酸二甲酯[cis-(±)-1],从而获得手性中间体 (S,S)-2,8-二氮杂双环壬烷,是合成氟喹诺酮类抗生素莫西沙星重要手性中间体的一条具有吸引力的工艺路线。在前期研究中,筛选得到了一株丝孢堆黑粉菌(SRL)来源的脂肪酶,该脂肪酶具有高热稳定性和pH值稳定性。但SRL来源的脂肪酶活性较低,无法满足工业化应用的需求。因此本研究依据前期南极假丝酵母来源脂肪酶B(CALB)改造策略,采用理性设计方法对SRL进行定点突变改造,获得一个双突变体SRLI194N/V195L。随后,确定了位于活性口袋外的环6 上两个关键氨基酸残基L145 和L154;获得四位点突变体SRL-I194N/V195L/L145V/L154G(V13),活性显著提高,达到87.8 U·mg−1,是野生型SRL的2195 倍(E > 200)。该突变体在50 ℃下的半衰期达到92.5 h。突变体V13(100 mg·L−1)能够高效拆分1 mol·L−1cis-(±)-1,2 h 内底物转化率达到49.9%,实现严格立体选择性(E > 200)。总体而言,研究发现了一株对cis-(±)-1 (S,S)-2,8-二氮杂双环壬烷具有高催化活性、严格立体选择性的脂肪酶,可应用于工业化生产,并为其他具有相似结构的脂肪酶和其他种类酶的活性提高提供了一种通用策略。

关键词: 脂肪酶     丝孢堆黑粉菌     定点突变     分子动力学模拟     理性设计     莫西沙星    

酯交换反应在制备生物柴油上的应用

张怀渊,宋元达

《中国工程科学》 2010年 第12卷 第1期   页码 24-29

摘要:

生物柴油因其环境友好且可再生作为矿物柴油的替代燃料而备受关注,生物柴油主要通过均相酸碱催化、脂肪酶催化、超临界法和固体酸碱催化的酯交换反应制备得到,但各种制备方法各有优劣,均相酸碱催化法反应迅速,转化率高但存在后续处理复杂,污染严重等问题;脂肪酶催化法反应条件温和,对原料中的水和游离脂肪酸不敏感,不需要过量的甲醇参与反应,后续处理工序简单,但酶的成本过高,这是制约其商业化发展的最大阻碍;超临界法是制备生物柴油的新技术,反应迅速,不需要催化剂,油脂转化率非常高,但其反应需要高温高压且能耗很大;固体酸碱催化剂

关键词: 生物柴油     酯交换反应     均相酸碱     固体酸碱     脂肪酶     超临界法    

标题 作者 时间 类型 操作

Study of ultrasound-promoted, lipase-catalyzed synthesis of fructose ester

Kai ZHU, Hui LIU, Pingfang HAN, Ping WEI,

期刊论文

Regioselective acylation of pyridoxine catalyzed by immobilized lipase in ionic liquid

BAI Shu, REN Mengyuan, WANG Lele, SUN Yan

期刊论文

糖尿病发作后心脏脂蛋白脂肪酶的变化

Chae Syng Lee, Yajie Zhai, Brian Rodrigues

期刊论文

Expression and characterization of a CALB-type lipase from

Jiang-Wei Shen, Xue Cai, Bao-Juan Dou, Feng-Yu Qi, Xiao-Jian Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

期刊论文

酶法合成sn-2棕榈酸甘油酯的研究进展

韦伟, 孙聪, 王小三, 金青哲, Xuebing Xu, Casimir C. Akoh, 王兴国

期刊论文

理性设计大幅提高一种丝孢堆黑粉菌来源脂肪酶催化活性——用于合成莫西沙星手性中间体

蔡雪, 沈江伟, 强煜, 华婧, 马樟奇, 柳志强, 郑裕国

期刊论文

酯交换反应在制备生物柴油上的应用

张怀渊,宋元达

期刊论文