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THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Jianping AN, Xiaofei WANG, Chunxiang YOU, Yujin HAO

《农业科学与工程前沿（英文）》页码 231-235 doi: 10.15302/J-FASE-2020367

摘要： Ascorbic acid (AsA, vitamin C) is involved in the regulation of many aspects of plant growth and development. It is an essential micronutrient for humans and can prevent scurvy, maintain the health of gums and blood vessels, reduce the level of plasma cholesterol and enhance the immune systen. Apple cultivars Orin and Guanghui were crossed to obtain a group of hybrid offspring with and without red flesh in the course of assessing apple germplasm resources. Unexpectedly, the red-flesh apples had higher AsA contents than other apples. Further studies showed that the anthocyanin biosynthetic regulator MdMYB1 directly activates the expression of dehydroascorbate reductase gene , thus promoting the activity of the DHAR enzyme and the accumulation of AsA. This finding reveals the mechanism leading to high AsA levels in red-flesh apples and suggests a new idea to cultivate red-flesh apples with high AsA contents and produce AsA efficiently and without pollution.

关键词： anthocyanin apples ascorbic acid MdMYB1 vitamin C

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Expression profiles of genes and enzymes related to ascorbic acid metabolism in fruits of

Yingying CHEN,Zhihui ZHAO,Jin ZHAO,Mengjun LIU

《农业科学与工程前沿（英文）》 2016年第3卷第2期页码 131-136 doi: 10.15302/J-FASE-2016096

摘要： The fruit of Chinese jujube ( ) possesses extremely high concentrations of ascorbic acid (AsA). The accumulation of AsA, the expression patterns of the nine genes related to AsA metabolism as well as the activities of five enzymes involved in AsA synthesis, oxidation and recycling were investigated during fruit development in Mill. ‘Jinsixiaozao’. The results showed that the high level of AsA accumulation in jujube fruit is due to a contribution from both AsA biosynthesis and AsA recycling. It is suggested that L-galactono-1,4-lactone dehydrogenase, ascorbate peroxidase and monodehydro-ascorbate reductase are the crucial genes/enzymes of jujube AsA synthesis, oxidization and recycling, respectively. These results provide useful new insights into the regulatory mechanisms of AsA accumulation in Chinese jujube.

关键词： Chinese jujube ascorbic acid metabolism gene enzyme

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THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

《农业科学与工程前沿（英文）》 2021年第8卷第2期

摘要：

Ascorbic acid (AsA, vitamin C) is involved in the regulation of many aspects of plant growth and development. It is an essential micronutrient for humans and can prevent scurvy, maintain the health of gums and blood vessels, reduce the level of plasma cholesterol and enhance the immune systen. Apple cultivars Orin and Guanghui were crossed to obtain a group of hybrid offspring with and without red flesh in the course of assessing apple germplasm resources. Unexpectedly, the red-flesh apples had higher AsA contents than other apples. Further studies showed that the anthocyanin biosynthetic regulator MdMYB1 directly activates the expression of dehydroascorbate reductase gene MdDHAR, thus promoting the activity of the DHAR enzyme and the accumulation of AsA. This finding reveals the mechanism leading to high AsA levels in red-flesh apples and suggests a new idea to cultivate red-flesh apples with high AsA contents and produce AsA efficiently and without pollution.

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Enhanced debromination of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) by zero-valent zinc with ascorbicacid

Chaojin Jiang, Xiaoqian Jiang, Lixun Zhang, Yuntao Guan

《环境科学与工程前沿（英文）》 2020年第14卷第3期 doi: 10.1007/s11783-020-1224-2

摘要： Highly efficient debromination of BDE-47 was achieved in the ZVZ/AA system. BDE-47 debromination by the ZVZ/AA can be applied to a wide range of pH. AA inhibits the formation of (hydr)oxide and accelerates the corrosion of ZVZ. Reduction mechanism of BDE-47 debromination by the ZVZ/AA system was proposed. A new technique of zero-valent zinc coupled with ascorbic acid (ZVZ/AA) was developed and applied to debrominate the 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47), which achieved high conversion and rapid debromination of BDE-47 to less- or non-toxic forms. The reaction conditions were optimized by the addition of 100 mg/L ZVZ particles and 3 mmol/L AA at original solution pH= 4.00 using the solvent of methanol/H2O (v:v= 4:6), which could convert approximately 94% of 5 mg/L BDE-47 into lower-brominated diphenyl ethers within a 90 min at the ZVZ/AA system. The high debromination of BDE-47 was mainly attributed to the effect of AA that inhibits the formation of Zn(II)(hydr)oxide passivation layers and promotes the corrosion of ZVZ, which leads to increase the reactivity of ZVZ. Additionally, ion chromatography and gas chromatography mass spectrometry analyses revealed that bromine ion and lower-debromination diphenyl ethers formed during the reduction of BDE-47. Furthermore, based on the generation of the intermediates products, and its concentration changes over time, it was proposed that the dominant pathway for conversion of BDE-47 was sequential debromination and the final products were diphenyl ethers. These results suggested that the ZVZ/AA system has the potential for highly efficient debromination of BDE-47 from wastewater.

关键词： 2 2′ 4 4′-tetrabromodiphenyl ether (BDE-47) Ascorbic acid Reductive debromination Zero-valent zinc

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Sulfonic acid-functionalized mesoporous silica catalyst with different morphology for biodiesel production

《化学科学与工程前沿（英文）》 2022年第16卷第8期页码 1198-1210 doi: 10.1007/s11705-021-2133-z

摘要： Sulfonic acid functionalized mesoporous silica based solid acid catalysts with different morphology were designed and fabricated. The synthesized materials were characterized by various physicochemical and spectroscopic techniques like scanning electron microscope-energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller surface area, thermogravimetric analysis and n-butylamine acidity. The shape of catalysts particles plays an important role in its activity. The sulfonic acid functionalized mesoporous silica catalysts of spherical shape and the cube shape were assessed for catalytic activity in biodiesel production. The catalytic biodiesel production reaction over the catalysts were studied by esterification of free fatty acid, oleic acid with methanol. The effect of various reaction parameters such as catalyst concentration, acid/alcohol molar ratio, catalyst amount, reaction temperature and reaction time on catalytic activity were investigated to optimize the conditions for maximum conversion. It was sulfonated cubic shape mesoporous silica which exhibited better activity as compared to the spherical shape silica catalysts. Additionally, the catalyst was regenerated and reused up to three cycles without any significant loss in activity. The present catalysts exhibit superior performance in biodiesel production and it can be used for the several biodiesel feedstock’s that are rich in free fatty acids.

关键词： solid acid catalyst mesoporous silica sulfonic acid biodiesel esterification oleic acid

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Amino acid promoted hydrogen battery system using Mn-pincer complex for reversible CO hydrogenation toformic acid

《能源前沿（英文）》 2022年第16卷第5期页码 697-699 doi: 10.1007/s11708-022-0843-7

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Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated

《化学科学与工程前沿（英文）》 doi: 10.1007/s11705-023-2361-5

摘要： In response to the reduction of food production and economic losses caused by plant bacterial diseases, it is necessary to develop new, efficient, and green pesticides. Natural products are rich and sustainable source for the development of new pesticides due to their low toxicity, easy degradation, and eco-friendliness. In this study, we prepared three series of ursolic acid derivatives and assessed their antibacterial ability. Most target compounds exhibited outstanding antibacterial activities. Among them, the relative optimal EC₅₀ values of Xanthomonas oryzae pv. oryzae and Xanthomonas axonopodis pv. citri were 2.23 (A17) and 1.39 (A16) μg·mL^–1, respectively. The antimicrobial mechanism showed that compound A17 induced an excessive accumulation and production of reactive oxygen species in bacteria and damaged the cell membrane integrity to kill bacteria. More interestingly, the addition of low concentrations of exogenous hydrogen peroxide enhanced the antibacterial efficacy of compound A17 against Xanthomonas oryzae pv. oryzae. These entertaining results suggested that compound A17 induced an apparent apoptotic behavior in the tested bacteria. Overall, we developed the promising antimicrobial agents that destroyed the redox system of phytopathogenic bacteria, further demonstrating the unprecedented potential of ursolic acid for agricultural applications.

关键词： ursolic acid antibacterial activities reactive oxygen species apoptosis

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Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability

《化学科学与工程前沿（英文）》 2023年第17卷第6期页码 784-794 doi: 10.1007/s11705-022-2278-4

摘要： In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts; however, it is limited by the conditions of metal–organic framework synthesis and its acid-base stability. Herein, a biocatalytic platform with improved acid-base stability was constructed via a one-pot method using bismuth-ellagic acid as the carrier. Bismuth-ellagic acid is a green phenol-based metal–organic framework whose organic precursor is extracted from natural plants. After encapsulation, the stability, especially the acid-base stability, of amyloglucosidases@bismuth-ellagic acid was enhanced, which remained stable over a wide pH range (2–12) and achieved multiple recycling. By selecting a suitable buffer, bismuth-ellagic acid can encapsulate different types of enzymes and enable interactions between the encapsulated enzymes and cofactors, as well as between multiple enzymes. The green precursor, simple and convenient preparation process provided a versatile strategy for enzymes encapsulation.

关键词： bismuth-ellagic acid in situ encapsulation enzyme@MOF biocomposites

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Abiotic association of phthalic acid esters with humic acid of a sludge landfill

Xiaoli CHAI, Yongxia HAO, Xin ZHAO, Guixiang LIU, Ying ZHU, Rong JI, Jun WU, Huanhuan TONG, Youcai ZHAO

《环境科学与工程前沿（英文）》 2012年第6卷第6期页码 778-783 doi: 10.1007/s11783-012-0434-7

摘要： The abiotic association between phthalic acid esters (PAEs) and humic substances (HS) in sludge landfill plays an important role in the fate and stability of PAEs. An equilibrium dialysis combined with C-labeling was used to study the abiotic association of two abundant PAEs (diethyl phthalate and di- -butyl phthalate) with humic acid (HA) isolated from a sludge landfill with different stabilization times and different molecular weights. Elemental analysis and Fourier Transform Infrared Spectrophotometer (FTIR) suggested that high value of HA was related to the high aromatic content and large molecular weight of HA. The results indicated that the association strength of PAEs with HA depended on both the properties of the PAEs and the characteristics of HA. The values of the association were strongly dependent on solution pH, and decreased dramatically as the pH was increased from 3.0 to 9.0. The results suggested that non-specific hydrophobic interaction between PAEs and HA was the main contributor to the association of the PAEs with HA. The interactive hydrogen-bonds between the HA and the PAEs molecules may also be involved in the association.

关键词： abiotic association phthalic acid esters (PAEs) humic acid sludge landfill

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Synergistic scale inhibition of polyaspartic acid composite with magnetic field

LIU Zhenfa, WANG Yanji, GAO Yuhua, ZHANG Lihui

《化学科学与工程前沿（英文）》 2007年第1卷第3期页码 261-265 doi: 10.1007/s11705-007-0047-z

摘要： Polyaspartic acid (PASP) composite, a scale inhibitor, was prepared from PASP, itaconic acid–acrylic acid–acrylic ester (IA-AA-AE) terpolymer and 2-phosphonobutane- 1,2,4-tricarboxylic acid (PBTCA). The scale inhibition of PASP composite and the effect of its synergism with a magnetic field on scale inhibition were investigated. Calcium carbonate crystals in scale samples were characterized by means of SEM and XRD. The static and dynamic experiments show that the chelating function of PASP composite for Ca can be enhanced by synergism with a magnetic field. Under Ca 650 mg/L, HCO 1,300 mg/L and PASP composite 4 mg/L, the scale inhibition rate of PASP composite in magnetic water can increase by 10% in static state and by 20% in dynamic experiments. Scanning electron microscope (SEM) and X-ray diffraction (XRD) results show that calcite and aragonite can be completely transformed into vaterite by using PASP composite.

关键词： synergism magnetic PBTCA dynamic acid–acrylic acid–acrylic

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Genomic and metabolomic analysis of with enhanced poly--glutamic acid production through atmospheric

《化学科学与工程前沿（英文）》 2022年第16卷第12期页码 1751-1760 doi: 10.1007/s11705-022-2211-x

摘要： Poly-γ-glutamic acid is an extracellular polymeric substance with various applications owing to its valuable properties of biodegradability, flocculating activity, water solubility, and nontoxicity. However, the ability of natural strains to produce poly-γ-glutamic acid is low. Atmospheric and room temperature plasma was applied in this study to conduct mutation breeding of Bacillus licheniformis CGMCC 2876, and a mutant strain M32 with an 11% increase in poly-γ-glutamic acid was obtained. Genome resequencing analysis identified 7 nonsynonymous mutations of ppsC encoding lipopeptide synthetase associated with poly-γ-glutamic acid metabolic pathways. From molecular docking, more binding sites and higher binding energy were speculated between the mutated plipastatin synthase subunit C and glutamate, which might contribute to the higher poly-γ-glutamic acid production. Moreover, the metabolic mechanism analysis revealed that the upregulated amino acids of M32 provided substrates for glutamate and promoted the conversion between L- and D-glutamate acids. In addition, the glycolytic pathway is enhanced, leading to a better capacity for using glucose. The maximum poly-γ-glutamic acid yield of 14.08 g·L^–1 was finally reached with 30 g·L^–1 glutamate.

关键词： ARTP mutagenesis Bacillus licheniformis poly-γ-glutamic acid metabolomics

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Untargeted metabolomic analysis of pregnant women exposure to perfluorooctanoic acid at different degrees

《环境科学与工程前沿（英文）》 2023年第17卷第3期 doi: 10.1007/s11783-023-1628-x

摘要：

● Metabolome can distinguish pregnant women exposure to PFOA at different degrees.

关键词： Perfluorooctanoic acid Exposure Pregnant women Metabolomic GSH Microbiota metabolism

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Design of nanocarriers for efficient cellular uptake and endosomal release of small molecule and nucleic acid

Vaibhav Mundra, Ram I. Mahato

《化学科学与工程前沿（英文）》 2014年第8卷第4期页码 387-404 doi: 10.1007/s11705-014-1457-3

摘要： There are many challenges in developing efficient and target specific delivery systems of small molecule and nucleic acid drugs. Cell membrane presents one of the major barriers for the penetration of hydrophilic macromolecules across the plasma membrane. Nanocarriers have been designed to enhance their cellular uptake via endocytosis but following their cellular uptake, endosomal escape is the rate limiting step which restricts the value associated with the enhanced uptake by nanocarriers. Viruses are an excellent model for efficient cytosolic delivery by nanocarriers. Viruses exploit intracellular cues to release the genome to cytosol. In this review, we first discuss different endocytic uptake pathways and endosomal escape mechanisms. We then summarize the existing tools for studying the intracellular trafficking of nanocarriers. Finally, we highlight the important design elements of recent virus-based nanocarriers for efficient cellular uptake and endosomal escape.

关键词： nanocarrier cellular uptake endosomal release nucleic acid drug

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Effects of humic acid on residual Al control in drinking water treatment plants with orthophosphate addition

Wendong WANG, Hua LI, Zhenzhen DING, Xiaochang WANG, Rui LIU

《环境科学与工程前沿（英文）》 2012年第6卷第4期页码 470-476 doi: 10.1007/s11783-012-0422-y

摘要： This study aimed to investigate the effects of humic acid (HA) on residual Al control in drinking water facilities that used orthophosphate addition. The results showed that adding orthophosphate was an effective method for residual Al control for the raw water without HA. When orthophosphate was added at 1.0 min before the addition of poly aluminum chloride (PACl), the concentrations of soluble aluminum (Sol-Al) and total aluminum (Tot-Al) residue were 0.08 and 0.086 mg·L , respectively; both were reduced by 46% compared with the control experiment. The presence of HA would notably increase the residual Al concentration. For the raw water with 5 mg·L of HA, the concentrations of Sol-Al and Tot-Al increased from 0.136 and 0.174 mg·L to 0.172 and 0.272 mg·L , respectively. For water with a HA concentration above 5 mg·L , orthophosphate was ineffective in the control of residual Al, though there were still parts of orthophosphate were removed in coagulation. The amounts of Al removal were positively correlated with the solids freshly formed in coagulation. Similar to the raw water without HA, the best Al control was obtained with orthophosphate salt added at 1.0 min before PACl. HA concentrations in the raw water, solution pH, and the orthophosphate dosage suitable for residual Al control by orthophosphate precipitation were also investigated.

关键词： coagulation drinking water humic acid orthophosphate residual Al

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Growth and abscisic acid responses of

Yue LI, Liqiang WAN, Yufei WANG, Xianglin LI

《农业科学与工程前沿（英文）》 2018年第5卷第1期页码 80-86 doi: 10.15302/J-FASE-2017199

摘要： A pot experiment was conducted in a greenhouse with three alfalfa ( ) cultivars, Aohan, Zhongmu No.1 and Sanditi, to examine the morphological and physiological responses of alfalfa to water stress. The response of alfalfa to water stress at different growth stages was generally similar, but varied among cultivars. At the branching, flowering and podding stages, the shoot biomasses of Aohan and Zhongmu No.1 were greatly affected by, and responded quickly to, water stress. The shoot biomass of Sanditi was not affected by mild water stress, but had a slight response to moderate and severe water stress. The root/shoot ratios in Aohan and Zhongmu No.1 were more sensitive to water stress than in Sanditi, with the root/shoot ratio in Aohan increasing most significantly. At flowering, the root/shoot ratio was the highest and the effect of water stress the greatest. The abscisic acid (ABA) concentration in the roots of Aohan and Zhongmu No.1 increased under water stress, while in Sanditi there was only a slight or delayed response of ABA concentration.

关键词： abscisic acid alfalfa drought response growth stage water deficit

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标题作者时间类型操作

THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Jianping AN, Xiaofei WANG, Chunxiang YOU, Yujin HAO

期刊论文

Expression profiles of genes and enzymes related to ascorbic acid metabolism in fruits of

Yingying CHEN,Zhihui ZHAO,Jin ZHAO,Mengjun LIU

期刊论文

THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

期刊论文

Enhanced debromination of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) by zero-valent zinc with ascorbicacid

Chaojin Jiang, Xiaoqian Jiang, Lixun Zhang, Yuntao Guan

期刊论文

Sulfonic acid-functionalized mesoporous silica catalyst with different morphology for biodiesel production

期刊论文

Amino acid promoted hydrogen battery system using Mn-pincer complex for reversible CO hydrogenation toformic acid

期刊论文

Discovery of novel ursolic acid derivatives as effective antimicrobial agents through a ROS-mediated

期刊论文

Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability

期刊论文

Abiotic association of phthalic acid esters with humic acid of a sludge landfill

Xiaoli CHAI, Yongxia HAO, Xin ZHAO, Guixiang LIU, Ying ZHU, Rong JI, Jun WU, Huanhuan TONG, Youcai ZHAO

期刊论文

Synergistic scale inhibition of polyaspartic acid composite with magnetic field

LIU Zhenfa, WANG Yanji, GAO Yuhua, ZHANG Lihui

期刊论文

Genomic and metabolomic analysis of with enhanced poly--glutamic acid production through atmospheric

期刊论文

Untargeted metabolomic analysis of pregnant women exposure to perfluorooctanoic acid at different degrees

期刊论文

Design of nanocarriers for efficient cellular uptake and endosomal release of small molecule and nucleic acid

Vaibhav Mundra, Ram I. Mahato

期刊论文

Effects of humic acid on residual Al control in drinking water treatment plants with orthophosphate addition

Wendong WANG, Hua LI, Zhenzhen DING, Xiaochang WANG, Rui LIU

期刊论文

Growth and abscisic acid responses of

Yue LI, Liqiang WAN, Yufei WANG, Xianglin LI

期刊论文

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