Search | Engineering

资源类型

期刊论文 2

年份

2015 1

关键词

检索范围：

排序：展示方式：

Can Food–Energy–Water Nexus Research Keep Pace with Agricultural Innovation?

Jie Zhuang,Tom Gill,Frank E. Löffler,Mingzhou Jin,Gary S. Sayler,

《工程（英文）》 doi: 10.1016/j.eng.2022.08.014

摘要： Highlights•Slow sectoral reorganization and imbalanced resource utilization strain sustainable adaptation of agriculture to emerging technologies.•Decentralized service can accelerate acceptance of FEW-converged technologies by farmers, investors, and policymakers.•FEW-converged technologies can reduce counterproductive impacts of non-comprehensive approach while increasing delivery of tangible solutions.

关键词： adaptation delivery FEW-converged technologies Decentralized service agriculture reorganization Agricultural Innovation?Highlights • Slow October acceptance policymakers.

HTML PDF 收藏

MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility

null

《医学前沿（英文）》 2015年第9卷第3期页码 331-343 doi: 10.1007/s11684-015-0409-8

摘要：

MicroRNAs (miRNAs), an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level. miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma (HCC) and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement. miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3′- and 5′-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.

关键词： hepatocellular carcinoma microRNA metastasis cytoskeletal reorganization