发布时间:
2021-03-01 00:00:00
期刊:
Food Chemistry
doi: 10.1016/j.foodchem.2020.127863
作者:
Manli Guo;Jingtian Chi;Chi Zhang;Minglin Wang;Hui Liang;Juying Hou;Shiyun Ai;Xiangyang Li
摘要:
In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/,) were obtained to detect lactose. Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability. Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together. X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8. Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite. Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/,, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.