Frontiers of Medicine
>> 2007,
Volume 1,
Issue 3
doi:
10.1007/s11684-007-0051-1
Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
1.Center for Healthy Food Evaluation, State Food and Drug Administration, Beijing 100061, China; 2.Institute for Nutritional Science, Shanghai Institute for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China; 3.Department of Medical Genetics, College of Basic Medical Science, Second Military Medical University, Shanghai 200433, China
Available online: 2007-09-05
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Abstract
The aim of this article was to investigate the influence and the related mechanism of the gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3b (GSK-3). The glucose uptake decreased with the increase in gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3 and p38MAPK, increased with overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways.
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