Journal Home Online First Current Issue Archive For Authors Journal Information 中文版

Frontiers of Medicine >> 2007, Volume 1, Issue 4 doi: 10.1007/s11684-007-0078-3

Correlative investigation of copper/low-density polyethylene nanocomposite on the endometrial angiogenesis in rats

1.Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2.Cancer Biology Research Center, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 3.Department of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

Available online: 2007-12-05

Next Previous

Abstract

The purpose of this study was to investigate the effects of copper/low-density polyethylene nanocomposite (nano-Cu/LDPE) on the endometrial angiogenesis in rats, and 100 sexual mature female SD rats were randomly divided into five groups: sham-operation groups (SO group, = 20), bulk copper groups (Cu group, = 20), LDPE groups ( = 20), nano-Cu/LDPE groups I ( = 20) and II ( = 20). The levels of angiopoietin-2 (Ang-2), its receptor (Tie-2) and CD34 of the rats endometria in each group were examined by using the S-P method of the immunohistochemistry 30 and 180 days after insertion, respectively. Compared with those in the SO group, the expression of Ang-2 and Tie-2 in all the experimental groups was obviously increased 30 days after insertion, and these parameters in nano-Cu/LDPE groups, except for Ang-2 level in nano-Cu/LDPE group II, were significantly lower in comparison with those in Cu group (<0.05). On the 180th day after insertion, Ang-2 and Tie-2 levels were still higher in Cu group and LDPE group, but there was no difference of Ang-2 and Tie-2 levels between nano-Cu/LDPE groups and the SO group (>0.05). Compared with those in the SO group, the significant increases in microvessel density (MVD) were observed on the 30th and the 180th day after the insertion of the bulk copper (<0.05). There was no significant difference in MVD counts before and after the insertion of nano-Cu/LDPE (>0.05). The results show that Nano-Cu/LDPE have slighter influence on the endometrial angiogenesis than bulk copper.

Related Research