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Frontiers of Medicine

2021, Volume 15,  Issue 1, Pages 144-154
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    Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

    . Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China;.. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing 100191, China;.. Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China;.. Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China;.. Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China;.. National Clinical Research Center of Obstetrics and Gynecology, Beijing 100191, China

    Received：2020-07-10 Accepted： 2020-09-01 Available online：2020-09-01
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    10.1007/s11684-020-0792-7
    Cite this article
    Ying Huo, Peng Yuan, Qingyuan Qin, Zhiqiang Yan, Liying Yan, Ping Liu, Rong Li, Jie Yan, Jie Qiao.Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes[J].Frontiers of Medicine,2021,15(1):144-154.

    Abstract

    Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30–32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.

    Keywords

    human metaphase II oocyte ; vitrification ; cryostorage duration ; single-cell RNA-Seq ; lncRNA
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