Frontiers of Chemical Science and Engineering
High-expression of recombinant human concensus interferon-α by Pichia pastoris
1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.Institute of Bioengineering, Academy of Military Medical Sciences, Beijing 100071, China
Available online: 2007-12-05
The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×10 U/mL, respectively.