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Frontiers of Chemical Science and Engineering >> 2009, Volume 3, Issue 4 doi: 10.1007/s11705-009-0247-9

Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3- O -sambubioside from the leaves of Nelumbo nucifera

State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang 330047, China;

Available online: 2009-12-05

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Quercetin-3--sambubioside Quercetin-3--β-D-xylopyranosyl (1→2)-β-D-glucopyranoside was separated and purified by semi-preparative high-speed counter-current chromatography (HSCCC) with a two-phase-solvent system composed of ethyl acetate-n-butanol–water (4∶1∶5, v/v) from the leaves of (Lotus). A total of 5.0mg of the targeted compound with a purity of 98.6% as determined by high performance liquid chromatography (HPLC) was obtained from 100m g of the crude extract cleaned up by AB-8 macroporous resin in a one-step separation. Quercetin-3--sambubioside was a novel flavonoid glycoside from the leaves of , and its chemical structure was identified by means of ESI-MS, 1D NMR and 2D NMR.

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