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2009, Volume 3, Issue 4

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Frontiers of Chemical Science and Engineering >> 2009, Volume 3, Issue 4 doi: 10.1007/s11705-009-0252-z

Bioproduction of D -psicose using permeabilized cells of newly isolated Rhodobacter sphaeroides SK011

1.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China；Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;

Available online: 2009-12-05
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Abstract

-psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of -psicose from -fructose. In this paper, another bacterium which could convert -fructose to -psicose was isolated and identified as . The process parameters of -psicose production using permeabilized cells of SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10min, and reaction conditions: cell concentration, 30g dry cell wt/L; concentration of substrate, 50g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5g/L -psicose with a -psicose productivity of 0.82g·L·h. This is the first report of bioproduction of -psicose using permeabilized cells of .

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