Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

2015, Volume 9, Issue 3

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Frontiers of Chemical Science and Engineering >> 2015, Volume 9, Issue 3 doi: 10.1007/s11705-015-1534-2

Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Institute of Chemical and Engineering Sciences, A*STAR, 1 Pesek Road, Jurong Island, 627833, Singapore

Accepted: 2015-09-24 Available online: 2015-09-30

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Abstract

Dry yeast cells (DYC) were used as a cheap nitrogen source to replace expensive yeast extract (YE) for L-lactic acid production by thermophilic . Cassava starch (200 g·L ) was converted to L-lactic acid by simultaneous saccharification and fermentation using WCP10-4 at 50 °C in the presence of 20 g·L of DYC, giving 148.1 g·L of L-lactic acid at 27 h with a productivity of 5.5 g·L ·h and a yield of 92%. In contrast, 154.4 g·L of lactic acid was produced at 24 h with a productivity of 6.4 g·L ·h and a yield of 96% when equal amount of YE was used under the same conditions. Use of pre-autolyzed DYC at 50 °C for overnight slightly improved the lactic acid titer (154.5 g·L ) and productivity (7.7 g·L ·h ) but gave the same yield (96%).

Keywords

L-lactic acid ; thermophilic strain ; Bacillus coagulans ; dry yeast cells ; autolysis ; fermentation

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