Frontiers of Chemical Science and Engineering
>> 2015,
Volume 9,
Issue 4
doi:
10.1007/s11705-015-1544-0
RESEARCH ARTICLE
Enhanced production of
1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China.2. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Henan 450002, China
Accepted: 2015-11-20
Available online: 2015-11-26
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Abstract
-Glucuronidase from Li-3 (PGUS) can efficiently hydrolyze glycyrrhizin into the more valuable glycyrrhetic acid monoglucuronide. However, a low productivity of PGUS and the lack of an effective separation strategy have significantly limited its industrial applications. Therefore, the production of PGUS has been improved by optimizing both the fermentation and purification strategies. A two-stage fermentation strategy was developed where PGUS was first grown with glucose and then PGUS was produced in the presence of glycyrrhizin as an inducer. By using this strategy, the biomass was increased 1.5 times and the PGUS activity increased 5.4 times compared to that when glycyrrhizin was used as the sole carbon source. The amount of PGUS produced was increased another 16.6% when the fermentation was expanded to a 15-L fermenter. An effective protocol was also established to purify the PGUS using a sequential combination of hydrophobic, strong anion-exchange and gel filtration chromatography. This protocol had a recovery yield of 6% and gave PGUS that was 39 times purer than the crude PGUS. The purified PGUS had a specific activity of 350 U·mg .
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