Genetic biosensors for small-molecule products: Design and applications in high-throughput screening

2017, Volume 11, Issue 1

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Frontiers of Chemical Science and Engineering >> 2017, Volume 11, Issue 1 doi: 10.1007/s11705-017-1629-z

Genetic biosensors for small-molecule products: Design and applications in high-throughput screening

. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200025, China.. University of the Chinese Academy of Sciences, Beijing 100049, China.. CAS Key Laboratory of Microbial Physiological and Metebolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.. Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China

Accepted: 2017-02-28 Available online: 2017-03-17

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Abstract

Overproduction of small-molecule chemicals using engineered microbial cells has greatly reduced the production cost and promoted environmental protection. Notably, the rapid and sensitive evaluation of the concentrations of the desired products greatly facilitates the optimization process of cell factories. For this purpose, many genetic components have been adapted into biosensors of small molecules, which couple the intracellular concentrations of small molecules to easily detectable readouts such as fluorescence, absorbance, and cell growth. Such biosensors allow a high-throughput screening of the small-molecule products, and can be roughly classified as protein-based and RNA-based biosensors. This review summarizes the recent developments in the design and applications of biosensors for small-molecule products.

Keywords

biosensor ; small molecule product ; transcription factor ; riboswitch ; high-throughput screening

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