In this study, fire tests of four single-section scaled-down utility tunnels were conducted. By analyzing temperature and structural responses of the utility tunnel throughout the fire exposure, the effects on the fire behavior of two different construction methods, cast-in-situ and prefabricated, and of two different materials, ordinary concrete and full lightweight concrete, were explored. The results of the study showed that the shear failure of the cast-in-situ utility tunnel occurred at the end of the top or bottom plate, and the failure of the prefabricated utility tunnel occurred at the junction of the prefabricated member and post-cast concrete. As the temperature increased, the temperature gradient along the thickness direction of the tunnel became apparent. The maximum temperature difference between the inner and outer wall surfaces was 531.7 °C. The highest temperature occurred in the cooling stage after stopping the heating, which provided a reference for the fire protection design and rescue of the utility tunnel. The displacement of the top plate of the prefabricated utility tunnel was 16.8 mm, which was 41.8% larger than that of the cast-in-situ utility tunnel. The bearing capacities of the ordinary concrete utility tunnel and full lightweight concrete utility tunnel after the fire loss were 27% and 16.8%, respectively. The full lightweight concrete utility tunnel exhibited good ductility and fire resistance and high collapse resistance.