Frontiers of Environmental Science & Engineering
>> 2013,
Volume 7,
Issue 4
doi:
10.1007/s11783-013-0498-z
RESEARCH ARTICLE
Selective pseudosolubilization capability of
College of Water Sciences, Beijing Normal University, Beijing 100875, China
Available online: 2013-08-01
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Abstract
Pseudosolubilized ability of sp. DG17 on -alkanes, role of biosurfactants in -octadecane uptake and trans-membrane transport mechanism of -octadecane were studied by analyzing amount of pseudosolubilized oil components in water phase, and the fraction of radiolabeled C -octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C to C of -alkanes. In -octadecane broth, pseudosolubilized -octadecane could be accumulated as long as pseudosolubilized rate was faster than mineralization rate of substrate, and the maximum concentration of pseudosolubilized -octadecane achieved to 45.37 mg·L . All of these results showed that sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of -octadecane.
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