2020, Volume 6, Issue 10
Engineering >> 2020, Volume 6, Issue 10 doi: 10.1016/j.eng.2020.07.013
SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface
a Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
b Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China
c Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China
d Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
e Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
f National Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China
g Beijing Advanced Innovation Center for Genomics, Beijing 100871, China
h Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Beijing 100191, China
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Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world, leading to large-scale population infection. Angiotensin-converting enzyme 2 (ACE2) is the receptor of both severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. However, it is still controversial whether vertical transmission exists. In order to investigate the potential risk of SARS-CoV-2 vertical transmission, we explored ACE2 and TMPRSS2 expression patterns in peri-implantation embryos and the maternal–fetal interface using previously published single-cell transcriptome data. The results show that day 6 (D6) trophectoderm cells (TE) in peri-implantation embryos, as well as 8-week syncytiotrophoblast (STB_8W) cells and 24-week extravillous trophoblast (EVTs_24W) cells in the maternal–fetal interface, strongly co-expressed ACE2 and TMPRSS2, indicating SARS-CoV-2 infection susceptibility. The ACE2-positive-expressing cells in the three cell types mentioned above share common characteristics, which are involved in autophagy and immune-related processes. ACE2 showed no gender bias in post-implantation embryos but showed a significant gender difference in D6 TE, D6 primitive endoderms (PE), and ACE2 positive-expressing STB cells. These findings indicate that there may be different SARS-CoV-2 infection susceptibilities of D6 embryos of different genders and during the gestation of different genders. Our results reveal potential SARS-CoV-2 infection risks during embryo transfer, peri-implantation embryo development, and gestation.
Keywords
SARS-CoV-2 ; ACE2 ; Vertical transmission ; Placenta ; Peri-implantation
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