2023, Volume 21, Issue 2
Engineering >> 2023, Volume 21, Issue 2 doi: 10.1016/j.eng.2021.12.007
Knockdown of a Specific Circular Non-coding RNA Significantly Suppresses Osteosarcoma Progression
a Musculoskeletal Tumor Center, Peking University People’s Hospital, Beijing 100044, China
b Beijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital, Beijing 100044, China
c Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
d State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
e Department of Chemistry, Tsinghua University, Beijing 100084, China
# These authors contributed equally to this work.
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Abstract
Osteosarcoma (OS) is a malignant mesenchymal tissue tumor known to occur in children and adolescents, and pulmonary metastasis often leads to death in these patients. The mechanism underlying OS progression remains unclear. Therefore, identifying new therapeutic targets and treatment modalities for OS is urgently needed. Abnormally expressed non-coding circular RNAs (circRNAs) are crucial for the occurrence and development of OS. The purpose of this study was to explore the expression and role of a novel circRNA circ_000203 in OS and elucidate the underlying mechanism. circ_000203 was demonstrated highly expressed in OS cell lines and tissues, and circ_000203 knockdown significantly inhibited OS progression in vitro and in vivo. Furthermore, we found that circ_000203 is a sponge of miR-26b-5p, an upstream regulator of bone morphogenetic protein receptor 2 (BMPR2). Thus, the overexpression of BMPR2 could reduce the inhibitory effect on OS progression. This indicates that knockdown of circ_000203 suppresses OS progression through microRNA (miRNA)-mediated BMPR2 downregulation. Our findings provide important insights for understanding the occurrence and development of OS.
Keywords
Non-coding RNA ; Osteosarcoma ; CircRNA ; Molecular mechanism ; Knockdown
SupplementaryMaterials
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