Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle

2014, Volume 1, Issue 3

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Frontiers of Agricultural Science and Engineering >> 2014, Volume 1, Issue 3 doi: 10.15302/J-FASE-2014031

Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle

1. Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom.2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Lanzhou730046, China

Available online: 2015-01-27

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Abstract

Foot-and-mouth-disease virus (FMDV) replicates in epithelial cells. The restriction of FMDV RNA to the basal cell layer of epithelia suggests a possible link between FMDV replication and the cell status. This paper describes studies in which FMDV infection was investigated in cells that were held at various cell division phases using cell cycle inhibitors. The results suggest that when cells were arrested at the G or G /S phase, high levels of viral RNA were detected by quantitative real-time reverse transcription PCR and viral protein synthesis was observed by specific labeling techniques. In contrast, when cells were arrested at the G /M phase, reduced or no viral RNA synthesis was detected.

Keywords

foot-and-mouth disease virus ; cell cycle ; replication

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