PtHAK5, a candidate for mediating high-affinity K

2018, Volume 5, Issue 1

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Frontiers of Agricultural Science and Engineering >> 2018, Volume 5, Issue 1 doi: 10.15302/J-FASE-2018200

PtHAK5, a candidate for mediating high-affinity K

State Key Laboratory of Grassland Agro-ecosystems/College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Accepted: 2018-02-08 Available online: 2018-03-21

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Abstract

is a typical salt-exclu-ding halophytic grass with strong salt-tolerance, which enhances tolerance by restricting Na influx as well as having a strong selectivity for K over Na . The HAK5 K transporters generally modulate effective K acquisition in plants, especially under low K condition. In this study, from was isolated by RT-PCR and characterized using yeast complementation. The results showed PtHAK5 consisted of 784 amino acids and shared over 80% homology with the identified high-affinity K transporter HAK5 from other higher plants. The expression of rescued the K -uptake-defective phenotype of yeast strain CY162. In conclusion, PtHAK5 is a candidate for mediating high-affinity K uptake under low K conditions.

Keywords

K⁺ uptake ; PtHAK5 ; Puccinellia tenuiflora ; yeast complementation

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