2020, Volume 7, Issue 4
Frontiers of Agricultural Science and Engineering >> 2020, Volume 7, Issue 4 doi: 10.15302/J-FASE-2019272
Optimizing hairy root production from explants of
. College of Horticulture and Forestry Sciences/Hubei Engineering Technology Research Center for Forestry Information, Huazhong Agricultural University, Wuhan 430070, China.. School of Environmental & Rural Science, The University of New England, Armidale, NSW 2351, Australia.. Wuhan Garden Constructional Engineering Company, Wuhan 430050, China.. Hubei Wildlife and Plant Conservation Agency, Wuhan 430070, China
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Abstract
is a shrub that has been used in traditional herbal medicine. It has great pharmaceutical potential for treating diseases such as cancer and diabetes. As a prerequisite for propagation of this species on a large scale, hairy roots in were induced using and various factors affecting hairy root induction and growth evaluated. Seven factors were tested: (1) type of explant, (2) type of culture medium, (3) duration of pre-culture, (4) inoculum cell density, (5) duration of infection, (6) acetosyringone concentration in the culture medium, and (7) duration of incubation. The optimal protocol for hairy root induction and growth was: young shoots, pre-cultured in Y1 for 2 d, inoculated with broth with an OD of 0.6 for 20 min, and incubated for 3 d. Putative transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain reaction. Successful and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of . Being able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.
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