Journal Home Online First Current Issue Archive For Authors Journal Information 中文版

Strategic Study of CAE >> 2009, Volume 11, Issue 5

Induced pluripotent stem cells (IPS): current situation and prospect

1. Institute of Basic Medicine, Chinese PLA General Hospital, Beijing 100853, China;

2. Trauma Center of Postgraduate Medical College, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037, China

Funding project:国家重点基础研究规划项目(2005CB522603);国家自然科学基金资助项目(30672176,30730090) Received: 2008-12-16

Next Previous

Abstract

Differentiated somatic cells can be reprogrammed into induced pluripotent stem cells (iPS) by transduction of a defined transcription factors. Undoubtedly, iPS-cell technology is a great breakthrough of stem cell research field in recent years. As a novel method for achieving an embryonic-like state without the ethical dilemma of destroying human embryos, iPS-cell technology elicit a great promise for patient-specific cell therapy and regenerative medicine. In addition, iPS-cell technology provides novel platforms for research of reprogramming mechanism and pathological process of human disease. However, the present development of pluripotent cells from individual somatic cells just opened up a new door to stem cell research world, much work remains to be done before iPS cells can be considered as a clinically reliable cell source. Here, the iPS cell research advances in recent years were reviewed and the feasible applications of iPS-cell technology were discussed.

Figures

图 1

图 2

References

[ 1 ] Takahashi K, Yamanaka S. Induction of p luripotent stem cells from mouse embryonic and adult fibroblast culture by defined factors [J ]. Cell, 2006, (126) : 663 - 676 link1

[ 2 ] Yu Junying, Vodyanik MA, Smuga - O tto K, et al. Induced p luripotent stem cell lines derived from human somatic cells [ J ]. Science, 2007, (318) : 1917 - 1920 link1

[ 3 ] WernigM, Meissner A, Foreman R, et al. In vitro rep rogramming of fibroblasts into a p luripotent ES - cell - like state [J ]. Nature, 2007, (448) : 318 - 324 link1

[ 4 ] Okita K, Ichisaka T, Yamanaka S. Generation of germline - competent induced p luripotent stem cells [ J ]. Nature, 2007, (448) : 313 - 317 link1

[ 5 ] Meissner A, WernigM, Jaenisch R. D irect rep rogramming of genetically unmodified fibroblasts into p luripotent stem cells [ J ]. Nature Biotechnol, 2007, (25) : 1177 - 1181 link1

[ 6 ] Hotta A, Ellis J. Retrial vector silencing during iPS cells induction: an ep igenetic beacon that signals distinct p luripotent states [ R ]. J Cell Biol, 2008, (105) : 940 - 948

[ 7 ] MacA rthur BD, Please CP, O reffo ROC. Stochasticity and molecular mechanisms of induced p luripotency [J ]. Plos One, 2008, (3) : 1 - 11

[ 8 ] M ikkelsen TS, Hanna J, Zhang Xiaolan, et al. D issecting direct rep rogramming through integrative genomic analysis [J ]. Nature, 2008, (454) : 49 - 55. link1

[ 9 ] Szutorisz H, Georgiou A, Tora L, et al. The p roteasome restricts permissive transcrip tion at tissue - specific gene loci in embryonic stem cells [J ]. Cell, 2006, (127) : 1375 - 1388 link1

[10] A rias AM, Hayward P. Flitering transcrip tional noise during development: concep ts and mechanisms [J ]. Nature Reviews Genetics, 2006, (7) : 34 - 44 link1

[11] Huangfu D, O safune K, Maehr R, et al. Induction of p luripotent stem cells frpm p rimary human fibroblasts with only Oct4 and Sox2 [J ]. Nature Biotechnol, 2008, (26) : 1269 - 1275 link1

[12] Liao Jing, Wu Zhao, Wang Ying, et al. Enhanced efficiency of generating induced p luripotent stem ( iPS) cells from human somatic cells by a combination of six transcrip tion factors [J ]. Cell Res, 2008, (18) : 600 - 603 link1

[13] Zhao Yang, Yin Xiaolei, Q in Han, et al. Two supporting factors greatly imp rove the efficiency of human iPSC generation [ J ]. Cell Stem Cell, 2008, (3) : 475 - 479 link1

[14] Stadtfeld M, Nagaya M, U tikal J, et al. Induced p luripotent stem cells generated without viral integration [ J ]. Science, 2008, (322) : 945 - 949 link1

[15] Kim JB, Zaehres H, Wu Guangming, et al. Pluripotent stem cells induced from adult neural stem cells by rep rogramming with two factors [J ]. Nature, (454) : 646 - 651 link1

[16] Eminli S, U tikal J, A rnold K, et al. Rep rogramming of neural p rogenitor cells into induced p luripotent stem cells in the absence of exogenous Soxexp ression [ J ]. Stem Cell, 2008, ( 26 ) : 2467 - 2474 link1

[17] Aasen T, Raya A, Barrero M, et al. Efficient and rap id generation of induced p luripotent stem cells from human keratinocytes [J ]. Nature Biotechnol, 2008, (26) : 1276 - 1284 link1

[18] Hanna J, Markoulaki S, Schorderet P, et al. D irect rep rogramming of terminally differentiated mature B lymphocytes to p luripotency [J ]. Cell, 2008, (133) : 250 - 264 link1

[19] Nakagawa M, Koyanagi M, Tanabe K, et al. Generation of induced p luripotent stem cells withoutMyc from mouse and human fibroblasts [J ]. Nature Biotechnol, 2008, (26) : 101 - 106 link1

[20] Shi Yan, Do JT, Desponts C, et al. A combined chemical and genetic app roach for the generation of induced p luripotent stem cells [J ]. Cell Stem Cell, 2008, (2) : 525 - 528 link1

[21] Silva J, Barrandon O, N ichols J, et al. Promotion of rep rogramming to ground state p luripotency by signal inhibition [ J ]. Plos Biology, 2008, (6) : 2237 - 2247 link1

[22] Marson A, Foreman R, Chevalier B, et al. Wnt signaling p romotes rep rogramming of somatic cells to p luripotency [ J ]. Cell Stem Cell, 2008, (3) : 13- 135 link1

[23] Shi Yan, Desponts C, Do JT, et al. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds [J ]. Cell Stem Cell, 2008, (3) : 568 - 574 link1

[24] Durcova - Hills G, Tang Fuchou, Doody G, et al. Rep rogramming p rimordial germ cells into p luripotent stem cells [ J ]. Plos One, 2008, (3) : 1 - 8 link1

[25] Rajasingh J, Lambers E, Hamada H, et al. Cell - free embryonic stem cell extract - mediated derivation ofmultipotent stem cells from N IH3T3 fibroblasts for functional and anatomical ischemic tissue repair [J ]. Circ Res, 2008, (102) : 107 - 117 link1

[26] Bru T, Clarke C, McGrewMJ, et al. Rap id induction of p luripotency genes after exposure of human somatic cells to mouse ES cell extracts [J ]. Exp Cell Res, 2008, (314) : 2634 - 2642 link1

[27] Fu Xiaobing, Sun Xiaoqing, Li Xiaokun, et al. Dedifferentiation of ep idermal cells to stem cells in vivo [ J ]. Lancet, 2001, (358) : 1067 - 1068 link1

[28] Cai Sa, Fu Xiaobing, Sheng Zhiyong. Dedifferentiation of human ep idermal cells induced without external gene intervention [ J ]. BioScience, 2007, (57) : 655 - 662

[29] O safune K, Caron L, Borowaik M, et al. Marked differences in differentiation p ropensity among human embryonic stem cell lines [J ]. Nature Biotechnol, 2008, (26) : 313 - 315 link1

[30] Adewumi O, Aflatoonian B, Ahrlund - RichterL, et al. Characterization of human embryonic stem cells lines by the international Stem Cell Initiative [J ]. Nature Biotechnol, 2007, ( 25) : 803 - 816 link1

[31] Hanna J, Wernig M, Markoulaki S, et al. Treatment of sickle cell anemia mouse modelwith iPS cells generated from autologous skin [J ]. Science, 2008, (21) : 1920 - 1923 link1

[32] D imos JT, Rodolfa KT, N iakan KK, et al. Induced p luripotent stem cells generated from patients with ALS can be differentiated into motor neurons [J ]. Science, 2008, (321) : 1218 - 1221 link1

[33] Tateishi K, He Jin, Taranova O, et al. Generation of insulin - secreting islet - like clusters from human skin fibroblasts [J ]. J Biol Chem, 2008, (283) : 31601 - 31607 link1

[34] Park H I, Arora N, Huo Hongguang, et al. D isease - specific induced p luripotent stem cells [ J ]. Cell, 2008, ( 134) : 877 - 886 link1

[35] Blelloch RH, Hochedlinger K, Yamada Y, et al. Nuclear cloning of embryonal carcinoma cells [J ]. Proc NatlAcid SciUSA, 2004, (101) : 13985 - 13990 link1

[36] Reya T, Morrison SJ, ClarkeMF, et al. Stem cells, cancer, and cancer stem cell [J ]. Nature, 2001, (414) : 105 - 111 link1

[37] Zhou Q iao, Brown J, Kanarek A, et al. In vivo rep rogramming of adult pancreatic exocrine cells to - cells [ J ]. Nature, 2008, (455) : 627 - 633 link1

[38] N ishikawa S, Goldstein RA, N ierras CR. The p romise of human induced p luripotent stem cells for research and therapy [J ]. Nature RevMol Cell Biol, 2008, (9) : 725 - 729 link1

Related Research