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Strategic Study of CAE >> 2013, Volume 15, Issue 1

Research and application on high-end equipment of biological detection and molecular diagnosis

1. School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China; 

2. State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, China;

3. Xi'an Tianlong Technology Co. Ltd., Xi'an 710049,China

Funding project:“863”国家高技术研究发展计划资助项目(2007AA042104; 2012AA040504);国家科技支撑计划资助项目(2012BAI19B05);国家自然科学基金资助项目(61227018) Received: 2012-10-10 Available online: 2013-01-14 15:42:09.000

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Abstract

Based on biological detection and molecular diagnostics technology development, the author summarized high-end equipment independent research in medical diagnostics, disease control, food safety, quarantine inspection, biology and medical research field of molecular diagnostics. The paper focused on research and development of key technology and instrumentation engineering technology, detection performance verification and successful application in the field of social development priorities of the real-time fluorescence quantitative polymerase chain reaction (PCR) nucleic acid detector, diagnosis of high-end equipment, the development and application and subsequent automatic nucleic acid directional monitoring with high-resolution analysis equipment development of handheld adenosine triphosphate (ATP) bioluminescence rapid detection of bacteria.

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References

[ 1 ] Mullis K B. The unusual origin of the polymerase chain reaction [J]. Scientific American,1990,262(4):56-61. link1

[ 2 ] Wittwer C T,Herman M G,Moss A A,et al. Continuous fluorescence monitoring of rapid cycle DNA amplification[J]. Biotechniques,1997,22(3):130-138. link1

[ 3 ] Mackay I M. Real- time PCR in the microbiology laboratory[J]. Clinical Microbiology and Infection,2004,10(3):190-212. link1

[ 4 ] Kubista M. Emerging real- time PCR applications[J]. Drug Discovery World,2008(summer):57-66.

[ 5 ] Life Technologies. PCR/RT- PCR[EB/OL]. [2005- 06]. https:// products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600961. link1

[ 6 ] Agilent Technologies. MX3000pBrochure.pdf[EB/OL]. [2004- 08]. www.bio.davidson.edu/courses/bio343/mx3000p_manual.pdf. link1

[ 7 ] 王 刚,明安杰,梁静秋. 光开关研究进展[J]. 微纳电子技术, 2005,42(4):195-199. link1

[ 8 ] 李 炜,彭年才,张镇西.光电倍增管(PMT)研究进展及在生命 科学领域的新应用[J].世界医疗器械,2005,11(5):76-79.

[ 9 ] Arlington. The market for real-time PCR reagents & instrumentation[J]. BioInformatics,2003(11):180.

[10] 彭年才,张镇西,兰邹然,等. 实时荧光定量PCR在甲型H1N1 流感诊断中的作用[J]. 世界医疗器械,2009,15(6):56-57.

[11] Wu Lihui,Zhao Qianlei,Zhu Xuchao,et al. A novel function of microRNA Let-7d in regulation of Galectin-3 expression in attention deficit hyperactivity disorder rat brain[J]. Brain Pathology,2010,20(6):1042-1054. link1

[12] 龚大江,刘家家,李春彬,等. 手持式ATP荧光检测仪的研制 [C]//中国生物医学工程进展——2007中国生物医学工程联合 学术年会论文集(上册).西安:西安交通大学出版社,2007:340-343. link1

[13] 彭年才,张镇西,刘家家,等. 一种手持式三磷酸腺苷荧光检测 装置:中国 200710017609.X [P]. 2008-03-12.

[14] 骆文静,侯 悦,胡艳冰,等. 铜银离子协同游离氯灭活水中微 生物的实验研究[J]. 解放军预防医学杂志,1997(5):326-329. link1

[15] Squirrell D J,Price R L,Murphy M J. Rapid and specific detection of bacteria using bioluminescence[J]. Analytica Chimica Acta,2002,457(1):109-114. link1

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