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Strategic Study of CAE >> 2014, Volume 16, Issue 9

The preparation and application of inactivated vaccine and its antiserum from eight common pathogens of Flatfish.

1. Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China;

2. Fishery Administration Bureau of Fushun,Fushun,Sichuan 643200,China

Funding project:现代农业产业技术体系建设专项资金(CARS-50) Received: 2014-07-09 Available online: 2014-09-16 08:54:39.000

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Abstract

In this study, formal dehyde was used to inactivate Edwardsiella tarda, Vibrio anguillarum, Vibrio harveyi, Vibrio vulnificus, Vibrio alginolyticus, Aeromonas caviae, Edwardsiella ictaluri, Aeromonas hydrophila, in order to preparethe inactivated vaccine. Then vaccine was used to immune turbot by intramuscular injection. In this way ,we got the antiserum of all bacterium above. Enzyme-linked immunosorbent assay was used to determine the content of antibody in serum. The antibody can be detect after four week in the immune, and the level of antibody is research highest in the tenth week which is inoculated inactivated vaccine of Edwardsiella tarda, and the highest titer of other vaccines are 1:102400, 1:6400, 1:6400, 1:12800, 1:12800, 1:6400, 1:6400, 1:12800. The results of cross reaction of the bacteria and their anti-serum shows that the effect is most intense when the bacteria react with its own antiserum, and it is very weak when the bacteria react with other antiserum.

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