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Mobile CRISPR-Cas9 based anti-phage system in E. coli

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8,   Pages 1281-1289 doi: 10.1007/s11705-022-2141-7

Abstract: Here, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coli

Keywords： phage infections     anti-phage     CRISPR-Cas9     conjugative transfer     synthetic biology    

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter of mice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression.

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 554-562 doi: 10.1007/s11684-017-0543-6

Abstract: successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9

Keywords： CAR-T     CRISPR-Cas9     LAG-3    

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PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing Article

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Engineering 2022, Volume 15, Issue 8,   Pages 67-77 doi: 10.1016/j.eng.2022.02.013

Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR) base editors allow for rapid, robustBy screening distinct CRISPR base editors, we discovered that only the unusual Streptococcus thermophilus CRISPR associated protein 9 (St1Cas9) cytosine base editor (CBE)—but not the widely usedStreptococcus pyogenes Cas9 (SpCas9) or Lachnospiraceae bacterium Cpf1 (LbCpf1) CBEsDespite the notable C-to-T conversions, a high proportion of undesired byproducts exists with St1Cas9

Keywords： CRISPR     Cas9     Mycobacterium tuberculosis     Genome editing     Base editing    

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One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 2-5 doi: 10.15302/J-FASE-2014007

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Genome-edited crops: how to move them from laboratory to market

Kunling CHEN, Caixia GAO

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 181-187 doi: 10.15302/J-FASE-2020332

Abstract:

Recent breakthroughs in CRISPR technology allow specific genome manipulation of almost all crops and

Keywords： CRISPR/Cas     genome editing     base editing     precision breeding     regulation    

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Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 148-160 doi: 10.15302/J-FASE-2019305

Abstract:

Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.

Keywords： animal breeding     cattle     cloning     CRISPR/Cas9     cytoplasmic injection     embryo     genome editing     germline chimaeras    

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First CRISPR-Based Therapy Poised to Reach Patients

Jennifer Welsh

Engineering 2023, Volume 30, Issue 11,   Pages 3-6 doi: 10.1016/j.eng.2023.09.005

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is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 690-704 doi: 10.1007/s11684-018-0638-8

Abstract: in hearing, we generated -null mice by clustered regularly interspaced short palindromic repeat/Cas9

Keywords： TPRN     stereocilia     stereociliary rootlet     actin filament     CRISPR/Cas9     hearing    

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CRISPR Narrows Gap between Genetically Altered Foods and Consumers

Sarah C.P. Williams

Engineering 2023, Volume 20, Issue 1,   Pages 6-8 doi: 10.1016/j.eng.2022.11.002

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Wheat Blast Pathogen Magnaporthe Oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Article

Houxiang Kang, Ye Peng, Kangyu Hua, Yufei Deng, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, Yilin Zhou, Xueping Zhou, Md Tofazzal Islam, Guo-Liang Wang

Engineering 2021, Volume 7, Issue 9,   Pages 1326-1335 doi: 10.1016/j.eng.2020.07.016

Abstract: Following this, we used the Cas12a protein and guide RNAs (gRNAs) to target the MoT-6098 and MoT-6099The activated Cas12a showed indiscriminate single-stranded DNase (ssDNAase) activity.We then combined target-dependent Cas12a ssDNase activation with recombinase polymerase amplification

Keywords： Wheat blast     Magnaporthe oryzae     Triticum Cas12a     Nucleic acid rapid lateral flow immunoassay     Field    

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Genome reprogramming for synthetic biology

Kylie Standage-Beier,Xiao Wang

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 37-45 doi: 10.1007/s11705-017-1618-2

Abstract: The ability to go from a digitized DNA sequence to a predictable biological function is central to synthetic biology. Genome engineering tools facilitate rewriting and implementation of engineered DNA sequences. Recent development of new programmable tools to reengineer genomes has spurred myriad advances in synthetic biology. Tools such as clustered regularly interspace short palindromic repeats enable RNA-guided rational redesign of organisms and implementation of synthetic gene systems. New directed evolution methods generate organisms with radically restructured genomes. These restructured organisms have useful new phenotypes for biotechnology, such as bacteriophage resistance and increased genetic stability. Advanced DNA synthesis and assembly methods have also enabled the construction of fully synthetic organisms, such as J. Craig Venter Institute (JCVI)-syn 3.0. Here we summarize the recent advances in programmable genome engineering tools.

Keywords： CRISPR     genome engineering     synthetic biology     rational design    

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Leader-following synchronization of a multi-agent system with heterogeneous delays Research Articles

Branislav Rehák, Volodymyr Lynnyk,rehakb@utia.cas.cz,volodymyr.lynnyk@utia.cas.cz

Frontiers of Information Technology & Electronic Engineering 2021, Volume 22, Issue 1,   Pages 1-140 doi: 10.1631/FITEE.2000207

Abstract: An algorithm is presented for leader-following synchronization of a composed of linear agents with . The presence of different delays in various agents can cause a synchronization error that does not converge to zero. However, the norm of this error can be bounded and this boundary is presented. The proof of the main results is formulated by means of linear matrix inequalities, and the size of this problem is independent of the number of agents. Results are illustrated through examples, highlighting the fact that the steady error is caused by heterogeneous delays and demonstrating the capability of the proposed algorithm to achieve synchronization up to a certain error.

Keywords： Multi-agent system     Time delay     Linear matrix inequality    

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CRISPR Use in Humans Shows Promise While Experts Weigh Ethical Concerns

Peter Weiss

Engineering 2020, Volume 6, Issue 7,   Pages 719-722 doi: 10.1016/j.eng.2020.05.010

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A PLATFORM TO AID SELECT THE OPTIMAL TOOL TO DESIGN GUIDE RNAS

Frontiers of Agricultural Science and Engineering   Pages 296-305 doi: 10.15302/J-FASE-2022479

Abstract:

● Summaries on sgRNAs design.

Keywords： CRISPR/Cas     Aid-TG     gene editing     sgRNA design     web server    

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Title Author Date Type Operation

Mobile CRISPR-Cas9 based anti-phage system in E. coli

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Journal Article

PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Journal Article

One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Journal Article

Genome-edited crops: how to move them from laboratory to market

Kunling CHEN, Caixia GAO

Journal Article

Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

Journal Article

First CRISPR-Based Therapy Poised to Reach Patients

Jennifer Welsh

Journal Article

is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

Journal Article

CRISPR Narrows Gap between Genetically Altered Foods and Consumers

Sarah C.P. Williams

Journal Article

Wheat Blast Pathogen Magnaporthe Oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated

Houxiang Kang, Ye Peng, Kangyu Hua, Yufei Deng, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, Yilin Zhou, Xueping Zhou, Md Tofazzal Islam, Guo-Liang Wang

Journal Article

Genome reprogramming for synthetic biology

Kylie Standage-Beier,Xiao Wang

Journal Article

Leader-following synchronization of a multi-agent system with heterogeneous delays

Branislav Rehák, Volodymyr Lynnyk,rehakb@utia.cas.cz,volodymyr.lynnyk@utia.cas.cz

Journal Article

CRISPR Use in Humans Shows Promise While Experts Weigh Ethical Concerns

Peter Weiss

Journal Article

A PLATFORM TO AID SELECT THE OPTIMAL TOOL TO DESIGN GUIDE RNAS

Journal Article