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Chao SUN, Meng LAI, Shougong ZHANG, Xiaomei SUN

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 4, Pages 482-492 doi: 10.15302/J-FASE-2017184

Abstract: Wood properties are important traits that determine quality of structural wood. With the aim of performing efficient early selection for wood properties, we investigated genetic variation in 20 clones aged from 4 to 15 years for four quality traits: wood density, wall thickness to lumen area, microfibrillar angle (MFA) and modulus of elasticity (MOE). We observed that age-related trends in overall means varied for different traits: MFA decreased with the age, while the others generally increased with the age. Phenotypic variance always showed significant differences from the age of 8 years onward, with CVG ranging from 4% to 25%. Also, clonal repeatability increased steadily until 9 years old and then kept medium or higher intensity (0.4–0.8). After the age of 6, genetic correlations were generally higher than phenotypic correlations. Estimates of early selection efficiency suggested that the optimal selection age for wood density was at age 5–6 years, while it was 9–10 years for the other traits. In combination with previous results, we proposed a comprehensive early selection strategy for larch clonal breeding that involved selection based on nursery rooting ability, phenology, growth traits, and wood properties.

Keywords： early selection genetic variation wood properties SilviScan Larix kaempferi

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Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1, Pages 77-84 doi: 10.15302/J-FASE-2014010

Abstract: While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of , a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of .

Keywords： Larix transcriptome age wood formation somatic embryogenesis molecular marker