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Journal Article 6

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2023 2

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2020 1

2019 2

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Metagenomics 5

Antibiotic resistance 1

Antibiotic resistance genes 1

Bacterial community 1

Bathyarchaeota 1

Comparative genomics 1

Culturomics 1

Decentralized sewage treatment facilities 1

Hyperthermophilic adaptation 1

Landfill leachate 1

Metabolomics 1

Network analysis 1

Partial nitrification-denitrification 1

Post-transplant diabetes mellitus 1

Process upgrade 1

Resistome 1

Tacrolimus 1

Virus 1

fresh corn straw 1

high solid anaerobic digestion 1

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Culturomics and metagenomics: In understanding of environmental resistome

Monika Nowrotek, Łukasz Jałowiecki, Monika Harnisz, Grażyna Anna Płaza

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1121-8

Abstract:

State of the art of culturomics and metagenomics to study resistome

The combination of culturomics and metagenomics

Keywords: Culturomics     Metagenomics     Antibiotic resistance     Resistome    

METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN

Frontiers of Agricultural Science and Engineering 2023, Volume 10, Issue 3,   Pages 403-423 doi: 10.15302/J-FASE-2022471

Abstract:

● Methane production from fresh straw was 7.50% higher than dry straw.

Keywords: fresh corn straw     high solid anaerobic digestion     metagenomics     microbial communities     thermophilic    

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized sewage treatment facilities

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 3, doi: 10.1007/s11783-021-1469-4

Abstract:

• Distribution of ARGs in decentralized sewage facilities were investigated.

Keywords: Decentralized sewage treatment facilities     Antibiotic resistance genes     Virus     Metagenomics     Network    

Core Metabolic Features and Hot Origin of Bathyarchaeota Article

Xiaoyuan Feng, Yinzhao Wang, Rahul Zubin, Fengping Wang

Engineering 2019, Volume 5, Issue 3,   Pages 498-504 doi: 10.1016/j.eng.2019.01.011

Abstract:

The archaeal phylum Bathyarchaeota comprises highly diversified subgroups and is considered to be one of the most abundant microorganisms on earth. The metabolic features and evolution of this phylum still remain largely unknown. In this article, a comparative metabolic analysis of 15 newly reconstructed and 36 published metagenomic assembled genomes (MAGs) spanning 10 subgroups was performed, revealing the core metabolic features of Bathyarchaeota—namely, protein, lipid, and benzoate degradation; glycolysis; and the Wood–Ljungdahl (WL) pathway, indicating an acetyl-CoA-centralized metabolism within this phylum. Furthermore, a partial tricarboxylic acid (TCA) cycle, acetogenesis, and sulfur-related metabolic pathways were found in specific subgroups, suggesting versatile metabolic capabilities and ecological functions of different subgroups. Intriguingly, most of the MAGs from the Bathy-21 and -22 subgroups, which are placed at the phylogenetic root of all bathyarchaeotal lineages and likely represent the ancient Bathyarchaeota types, were found in hydrothermal environments and encoded reverse gyrase, suggesting a hyperthermophilic feature. This work reveals the core metabolic features of Bathyarchaeota, and indicates a hot origin of this archaeal phylum.

Keywords: Bathyarchaeota     Metagenomics     Comparative genomics     Hyperthermophilic adaptation    

Taxonomic and functional variations in the microbial community during the upgrade process of a full-scale landfill leachate treatment plant – from conventional to partial nitrification-denitrification

Binbin Sheng, Depeng Wang, Xianrong Liu, Guangxing Yang, Wu Zeng, Yiqing Yang, Fangang Meng

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 6, doi: 10.1007/s11783-020-1272-7

Abstract: Abstract • Upgrade process was investigated in a full-scale landfill leachate treatment plant. • The optimization of DO can technically achieve the shift from CND to PND process. • Nitrosomonas was mainly responsible for ammonium oxidation in PND system. • An obviously enrichment of Thauera was found in the PND process. • Enhanced metabolic potentials on organics was found during the process update. Because of the low access to biodegradable organic substances used for denitrification, the partial nitrification-denitrification process has been considered as a low-cost, sustainable alternative for landfill leachate treatment. In this study, the process upgrade from conventional to partial nitrification-denitrification was comprehensively investigated in a full-scale landfill leachate treatment plant (LLTP). The partial nitrification-denitrification system was successfully achieved through the optimizing dissolved oxygen and the external carbon source, with effluent nitrogen concentrations lower than 150 mg/L. Moreover, the upgrading process facilitated the enrichment of Nitrosomonas (abundance increased from 0.4% to 3.3%), which was also evidenced by increased abundance of amoA/B/C genes carried by Nitrosomonas. Although Nitrospira (accounting for 0.1%–0.6%) was found to stably exist in the reactor tank, considerable nitrite accumulation occurred in the reactor (reaching 98.8 mg/L), indicating high-efficiency of the partial nitrification process. Moreover, the abundance of Thauera, the dominant denitrifying bacteria responsible for nitrite reduction, gradually increased from 0.60% to 5.52% during the upgrade process. This process caused great changes in the microbial community, inducing continuous succession of heterotrophic bacteria accompanied by enhanced metabolic potentials toward organic substances. The results obtained in this study advanced our understanding of the operation of a partial nitrification-denitrification system and provided a technical case for the upgrade of currently existing full-scale LLTPs.

Keywords: Landfill leachate     Process upgrade     Partial nitrification-denitrification     Bacterial community     Metagenomics    

Alterations in the Gut Microbiome in Liver Recipients with Post-transplant Diabetes Mellitus Article

Qi Ling, Yuqiu Han, Yue Ma, Xiaosen Wang, Zheng Zhu, Jingyu Wang, Jiaying Cao, Lin Xiaohan, Jun Wang, Baohong Wang

Engineering 2023, Volume 31, Issue 12,   Pages 98-111 doi: 10.1016/j.eng.2023.09.006

Abstract: Here, by combining high-throughput shotgun metagenomics sequencing and metabolomics profiling, we characterized

Keywords: Post-transplant diabetes mellitus     Tacrolimus     Metagenomics     Metabolomics    

Title Author Date Type Operation

Culturomics and metagenomics: In understanding of environmental resistome

Monika Nowrotek, Łukasz Jałowiecki, Monika Harnisz, Grażyna Anna Płaza

Journal Article

METAGENOMICS COMBINED WITH HIGH-THROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN

Journal Article

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized sewage treatment facilities

Journal Article

Core Metabolic Features and Hot Origin of Bathyarchaeota

Xiaoyuan Feng, Yinzhao Wang, Rahul Zubin, Fengping Wang

Journal Article

Taxonomic and functional variations in the microbial community during the upgrade process of a full-scale landfill leachate treatment plant – from conventional to partial nitrification-denitrification

Binbin Sheng, Depeng Wang, Xianrong Liu, Guangxing Yang, Wu Zeng, Yiqing Yang, Fangang Meng

Journal Article

Alterations in the Gut Microbiome in Liver Recipients with Post-transplant Diabetes Mellitus

Qi Ling, Yuqiu Han, Yue Ma, Xiaosen Wang, Zheng Zhu, Jingyu Wang, Jiaying Cao, Lin Xiaohan, Jun Wang, Baohong Wang

Journal Article