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Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1, Pages 21-27 doi: 10.1007/s11783-011-0303-9
Keywords: polymerase chain reaction (PCR) microbial communities pyrosequencing gut microbial fuel cell sludge
Measurement and Control Technology for the Array of Low Cost PCR Microreactors
Li Xianming ,Zhang Yulin ,Li Jiantian
Strategic Study of CAE 2007, Volume 9, Issue 7, Pages 76-81
In this paper a new style of polymerase chain reaction(PCR)equipmentIt is the concrete way to reduce the cost of PCR chips and improve the reliability of the chips.The measurement and control scheme for the low-cost PCR chips is designed.The uncertainty of indirect to the temperature of PCR liquid in low-cost PCR chips is also assessed.Its performance in temperature cycling meets the requirements of PCR.
Keywords: microreactors measurement control polymerase chain reaction
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 3, Pages 488-488 doi: 10.1007/s11783-011-0342-2
Safety evaluation of microbial pesticide (HaNPV) based on PCR method
Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian
Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 2, Pages 377-384 doi: 10.1007/s11705-018-1777-9
Keywords: microbial pesticides HaNPV acute intravenous injection infectivity pathogenicity
Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,
Frontiers of Medicine 2010, Volume 4, Issue 2, Pages 204-207 doi: 10.1007/s11684-010-0043-4
Keywords: Angiostrongylus cantonensis Pomacea canaliculata multiplex polymerase chain reaction diagnosis pooling
Research and application on high-end equipment of biological detection and molecular diagnosis
Peng Niancai,Li Lei,Li Zheng,Miao Baogang,Li Ming,Zhao Yao,Jiang Zhuangde
Strategic Study of CAE 2013, Volume 15, Issue 1, Pages 57-62
Keywords: biological detection molecular diagnosis real-time quantitative PCR ATP luminescence detection
Qiangru Huang, Yanxia Sun, Mengmeng Jia, Ting Zhang, Fangyuan Chen, Mingyue Jiang, Qing Wang, Luzhao Feng, Weizhong Yang
Engineering 2023, Volume 28, Issue 9, Pages 237-245 doi: 10.1016/j.eng.2023.01.004
Keywords: COVID-19 Antigen testing PCR testing Testing strategies
Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao
Strategic Study of CAE 2014, Volume 16, Issue 9, Pages 50-60
Keywords: Cynoglossus semilaevis Günther LH cDNA cloning Quantitative real-time PCR RIA
Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI
Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 5, Pages 693-702 doi: 10.1007/s11783-014-0635-3
Keywords: ammonia-oxidizing archaea ammonia-oxidizing bacteria quantitative PCR clone library plateau
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2, Pages 175-185 doi: 10.1007/s11783-011-0320-8
Keywords: quantitative polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) principal
Assessment of antibiotic resistance genes in dialysis water treatment processes
Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng
Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1136-1
• Quantitative global ARGs profile in dialysis water was investigated. • Totally 35 ARGs were found in the dialysis treatment train. • 29 ARGs (highest) were found in carbon filtration effluent. • erm and mtrD-02 occurred in the final effluent. • The effluent was associated with health risks even after RO treatment.
Keywords: Dialysis water Treatment process Antibiotic resistance gene High-throughput quantitative PCR Horizontal
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1, Pages 40-47 doi: 10.1007/s11783-011-0304-8
Keywords: sewage treatment plants polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
Frontiers of Medicine 2008, Volume 2, Issue 3, Pages 283-285 doi: 10.1007/s11684-008-0053-7
Keywords: D-HPLC mutation development autoimmune PCR-RFLP candidate
Nucleic acid-based diagnostics for infectious diseases in public health affairs
Albert Cheung-Hoi Yu, Greg Vatcher, Xin Yue, Yan Dong, Mao Hua Li, Patrick H. K. Tam, Parker Y.L. Tsang, April K.Y. Wong, Michael H.K. Hui, Bin Yang, Hao Tang, Lok-Ting Lau
Frontiers of Medicine 2012, Volume 6, Issue 2, Pages 173-186 doi: 10.1007/s11684-012-0195-5
Infectious diseases, mostly caused by bacteria and viruses but also a result of fungal and parasitic infection, have been one of the most important public health concerns throughout human history. The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost. Many kinds of diagnostics have been developed, such as pathogen culture, biochemical tests and serological tests, to help detect and fight against the causative agents of diseases. However, these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results. Nucleic acid-based diagnostics, detecting pathogens through the identification of their genomic sequences, have shown promise to overcome the above limitations and become more widely adopted in clinical tests. Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage. We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics.
Keywords: nucleic acid-based diagnostics infectious disease PCR NASBA LAMP microarray LOAC public health affairs
Hanjiang Lai, Chen Huang, Jian Cai, Julian Ye, Jun She, Yi Zheng, Liqian Wang, Yelin Wei, Weijia Fang, Xianjun Wang, Yi-Wei Tang, Yun Luo, Dazhi Jin
Frontiers of Medicine 2018, Volume 12, Issue 2, Pages 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CEstrains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR
Keywords: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
Title Author Date Type Operation
Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
Journal Article
Measurement and Control Technology for the Array of Low Cost PCR Microreactors
Li Xianming ,Zhang Yulin ,Li Jiantian
Journal Article
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
Journal Article
Safety evaluation of microbial pesticide (HaNPV) based on PCR method
Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian
Journal Article
A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host
Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,
Journal Article
Research and application on high-end equipment of biological detection and molecular diagnosis
Peng Niancai,Li Lei,Li Zheng,Miao Baogang,Li Ming,Zhao Yao,Jiang Zhuangde
Journal Article
Quantitative Analysis of the Effectiveness of Antigen- and Polymerase Chain Reaction-Based Combination Strategies for Containing COVID-19 Transmission in a Simulated Community
Qiangru Huang, Yanxia Sun, Mengmeng Jia, Ting Zhang, Fangyuan Chen, Mingyue Jiang, Qing Wang, Luzhao Feng, Weizhong Yang
Journal Article
Full length cDNA cloning and expression of luteinizing hormone (LH) and which serum concentration was measured in half smooth tongue sole Cynoglossus semilaevis Günther
Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao
Journal Article
Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China
Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI
Journal Article
Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
Journal Article
Assessment of antibiotic resistance genes in dialysis water treatment processes
Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng
Journal Article
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
Journal Article
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
Journal Article
Nucleic acid-based diagnostics for infectious diseases in public health affairs
Albert Cheung-Hoi Yu, Greg Vatcher, Xin Yue, Yan Dong, Mao Hua Li, Patrick H. K. Tam, Parker Y.L. Tsang, April K.Y. Wong, Michael H.K. Hui, Bin Yang, Hao Tang, Lok-Ting Lau
Journal Article