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Mobile CRISPR-Cas9 based anti-phage system in E. coli

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8,   Pages 1281-1289 doi: 10.1007/s11705-022-2141-7

Abstract: Here, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coliand pGM2 carrying one and two guide RNAs, respectively, were designed to defend against a filamentous phageFor preventing phage infection in E. coli, the pGM2 decreased the phage infection rate to 0.1%, while pGM1 failed to block phage infection.results support the potential application of MCBAS in E. coli cell factories to defend against phage

Keywords: phage infections     anti-phage     CRISPR-Cas9     conjugative transfer     synthetic biology    

Bacteriophages in water pollution control: Advantages and limitations

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 5, doi: 10.1007/s11783-020-1378-y

Abstract:

•Phages can be better indicators of enteric viruses than fecal indicator bacteria.

Keywords: Phage     Water pollution monitoring     Harmful bacteria biocontrol     Horizontal gene transfer     Bacterial resistance    

Title Author Date Type Operation

Mobile CRISPR-Cas9 based anti-phage system in E. coli

Journal Article

Bacteriophages in water pollution control: Advantages and limitations

Journal Article